Microfluidics study of intracellular calcium response to mechanical stimulation on single suspension cells

Xu, Tao; Yue, Wanqing; Li, Cheuk‐Wing; Yao, Xin‐Sheng; Yang, Mengsu

doi:10.1039/c3lc40880a

Cited by 22 publications

(20 citation statements)

References 31 publications

(41 reference statements)

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“…197 Finally, the intracellular calcium response of suspension leukemic cells to mechanical stimulation was examined by designing a device in which the cells were compressed under controlled conditions within a channel manifold. 198 …”

Section: Applicationsmentioning

confidence: 99%

Micro Total Analysis Systems: Fundamental Advances and Biological Applications

et al. 2013

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Section: Applicationsmentioning

confidence: 99%

Micro Total Analysis Systems: Fundamental Advances and Biological Applications

et al. 2013

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“…The sandbag structures (Fig. 8.4c) were utilized to immobilize microbeads and cells for high-throughput assays [99,100] and cell communication studies [101].…”

Section: Manipulation Of Microbeads In Microfluidicsmentioning

confidence: 99%

Microfluidics for DNA and Protein Analysis with Multiplex Microbead-Based Assays

Yue

Yang

2016

Microfluidic Methods for Molecular Biology

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Early screening and diagnosis of diseases require the development of sensitive, reliable, and inexpensive high-throughput assays. There is a growing need for innovative diagnostic technologies that provide accurate detection of a broad range of diseases and enhance laboratory productivity. Developments in micro-and nanotechnology have advanced the "lab-on-a-chip" concept towards a new generation of point-of-care diagnostic devices that enable parallel detection of multiple analytes in small-volume samples with high sensitivity in a short time. These features fulfill some of the important criteria of bioanalysis used for biochemical studies, environmental analyses, and clinical diagnostics. However, the multiplexing capability of microfluidic-based assay is limited compared to conventional flow cytometric assays, particularly for encoded microbead-based assays, which are capable of simultaneously analyzing multiple analyte. It is possible to incorporate the microbead-based assays into microfluidic devices in order to retain all the advantages of the microdevice and significantly improve multiplexing capability. In this chapter, we summarize the latest development in microbeadbased assays and discuss the integration of the microbead technology with microfluidic devices. Applications of the integrated approach in multiplexed protein and DNA analysis are growing rapidly in the areas of biomarker research, cancer screening, and disease diagnosis. The prospects for future development and commercialization of these microbead-based microfluidic devices are also discussed.

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“…In order to study cellular response to compression (Figure 3c), a chip that produces cellular lysis was designed to facilitate on chip cell-based analysis [86]. Compression studies in a chip with individual leukemic cells showed that extracellular calcium uptake was upregulated in stimulated cells [96]. Furthermore, mechanical compression has been used to investigate axonal degeneration after compression trauma [97] and for some diseases such as osteoarthritis [98].…”

Section: Manipulation and Sensing Of Microfluidic 3d Hydrogel Culturementioning

confidence: 99%

Recent Advances of Biologically Inspired 3D Microfluidic Hydrogel Cell Culture Systems

Ertl¹

2015

CBCM

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Application of Microfluidics in Life SciencesMicrofluidics can be defined as the study of fluid and air flows in microchannels and was initially introduced to facilitate liquid handling and sample preparations. Early work dates back to 1969 with Lew's work on a theoretical solution for mimicking blood and air flow in a microcirculatory system of the lung [1]. In this precursor stage of microfluidics the aim was to create a biomimetic system, which facilitated the study of biological pathways in vitro. It was not until the 1990s that the field of microfluidics emerged from miniaturization efforts and Micro-Electro-Mechanical Systems (MEMS) as an enabling technology platform for dispensing systems, analytical separations, chemical reactions, and bioanalysis applications. Since then, microfluidics has evolved into an established technology ranging from medical solutions (e.g., microfluidic inhalers), in vitro diagnostics (e.g., point of care) and production applications (e.g., microreaction technologies) [2]. More recent research applications include microchips for genomics, proteomics and cell-based assays.These microfluidic cell cultures are considered potential candidates to provide next generation cell analysis systems. Starting from single cell analysis using miniaturized flow cytometers [3] a variety of microfluidic devices have been developed for cell studies to investigate cell transport and cultivation in the absence and presence of concentration and temperature gradients or shear force conditions. The main benefit of microfluidic systems for cell culture analysis is that they can perform a number of crucial liquid handling steps including cell loading, nutrient supply and waste removal under physiologically relevant shear force conditions, all while offering real time microscopy [4]. Microfluidics also enables precise regulation of soluble factors including drug candidates, growth factors at specific solution concentrations and gradients, thus providing robust and reproducible measurement conditions. An alternative application of microfluidics for cell analysis is micropatterning to (a) optimize control of cellular behavior [5], (b) allow cell migration [6], (c) spatially resolve co-cultures systems [7] and (d) define cell repulsive and adhesive areas [8].Despite recent achievements of microfluidic 2D cell culture systems [9], they still do not address the fact that in vivo cells coexist in 3D communities that are influenced by spatial orientation of cells and cell-to-cell contact within the extracellular matrix [10]. It has been repeatedly demonstrated that the presence of a 3D matrix promotes many biologically relevant functions otherwise not observed in 2D monolayer cell cultures [11]. Consequently a transition from 2D to 3D cell cultures has gained momentum as an increasing number of reports have confirmed significant differences in the morphology, protein expression, differentiation, migration, functionality and viability of cells between 3D and 2D cell cultures [12]; these non-microfluidic advances a...

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Microfluidics study of intracellular calcium response to mechanical stimulation on single suspension cells

Cited by 22 publications

References 31 publications

Micro Total Analysis Systems: Fundamental Advances and Biological Applications

Micro Total Analysis Systems: Fundamental Advances and Biological Applications

Microfluidics for DNA and Protein Analysis with Multiplex Microbead-Based Assays

Recent Advances of Biologically Inspired 3D Microfluidic Hydrogel Cell Culture Systems

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