Identification of disulphide stress–responsive extracytoplasmic function sigma factors in Rothia mucilaginosa

Nambu, Takayuki; Yamane, Kazuyoshi; Yamanaka, Takeshi; Mashimo, Chiho; Maruyama, Hugo; Yoshida, Masahiro; Hayashi, Hiroyuki; Leung, Kai P.; Fukushima, Hajime

doi:10.1016/j.archoralbio.2012.10.017

Cited by 4 publications

(4 citation statements)

References 45 publications

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“…The HxxxC motif has been found, or proposed, in a few proteins for binding iron-sulfur cluster or zinc ion. [52][53][54][55] A similar, CxxHx 16 H heme-binding motif has been reported in the K ATP channel protein, whose ion channel opens in response to the binding of carbon monoxide. 56 A signicant difference is that the cysteine residue in this motif is an axial ligand of the heme cofactor which does not form a covalent link to a vinyl group of the heme, as observed in this study of SfmD.…”

Section: Discussionmentioning

confidence: 87%

A novel catalytic heme cofactor in SfmD with a single thioether bond and a bis-His ligand set revealed by a de novo crystal structural and spectroscopic study

et al. 2021

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Section: Discussionmentioning

confidence: 87%

A novel catalytic heme cofactor in SfmD with a single thioether bond and a bis-His ligand set revealed by a de novo crystal structural and spectroscopic study

et al. 2021

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“…The R. mucilaginosa genome codes for several σ factors that are upregulated in response to oxidative stress. 13 In addition, R. mucilaginosa can generate acetaldehyde from ethanol; increased levels of acetaldehyde are capable of inducing oxidative stress, 47 suggesting that R. mucilaginosa has increased resistance to either acetaldehyde or the resultant oxidative stress it induces. This factor, combined with the thick peptidoglycan cell wall, may explain the variation in efficacy between the 2 organisms we studied.…”

Section: Discussionmentioning

confidence: 99%

“… 10 , 11 In biofilms such as dental plaques, this gram-positive, encapsulated, nonmotile, bacterium forms multispecies microcolonies or, in the case of high-biomass regions, small islands 12 and shows resilience even after multiple endodontic treatments. 13 Although R. mucilaginosa is typically nonpathogenic in healthy individuals, it can act as an opportunistic pathogen and lead to conditions such as pneumonia, 14 bacteremia, 15 and endocarditis, 16 and cause the buildup of biofilms on prosthetics, 17 among others. 18 …”

Section: Introductionmentioning

confidence: 99%

Biofilm disruption and bactericidal activity of aqueous ozone coupled with ultrasonic dental scaling

Failor¹,

Silver²,

Yu³

et al. 2022

JADA Foundational Science

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Background The COVID-19 pandemic has heightened the awareness of a common hazard encountered in the dental clinic: aerosol transmission of pathogens. Treatment of sources of infection before or during dental procedures is one means of decreasing pathogen load and aerosol transmission. Methods An ultrasonic scaler supplied with aqueous ozone was used to examine the effect of its viability on planktonic cultures and biofilms formed by 2 model bacteria: Rothia mucilaginosa and Escherichia coli . Results Both organisms showed susceptibility to aqueous ozone alone (97% and 99.5% lethality, respectively). When combined with manual scaling using an ultrasonic scaler, a greater than 99% reduction in colony-forming units (CFUs)/mL could be reached with an aqueous ozone concentration of approximately 2 mg/L ( R. mucilaginosa ) or 0.75 mg/L ( E. coli ) after 5 through 6 seconds of scaling. Conclusions Aqueous ozone coupled with ultrasonic scaling exhibited a higher efficiency of microbial kill than either method used alone . Both gram-positive and gram-negative species were affected by this treatment. Studies on other oral microbiota constituents, including fungi and viruses, will provide information on the efficacy of this method on a greater biological scale. Studies to verify concomitant reduction of microbial load in dispersed aerosols in clinical settings should be completed to support practical applications of this treatment.

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“…AP011540) ( 8 ). The genome of DY-18 encodes two sigma factors, one of which is an extracytoplasmic function (ECF) sigma factor markedly up-regulated under disulfide stress ( 9 ), but this strain is not amenable to genetic transformation. Therefore, we isolated a transformable strain of R. mucilaginosa (NUM-Rm6536) from tongue plaque of a healthy human adult (our unpublished data) ( 10 ).…”

Section: Genome Announcementmentioning

confidence: 99%

Complete Genome Sequence of Rothia mucilaginosa Strain NUM-Rm6536, Isolated from a Human Oral Cavity

et al. 2015

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Identification of disulphide stress–responsive extracytoplasmic function sigma factors in Rothia mucilaginosa

Cited by 4 publications

References 45 publications

A novel catalytic heme cofactor in SfmD with a single thioether bond and a bis-His ligand set revealed by a de novo crystal structural and spectroscopic study

A novel catalytic heme cofactor in SfmD with a single thioether bond and a bis-His ligand set revealed by a de novo crystal structural and spectroscopic study

Biofilm disruption and bactericidal activity of aqueous ozone coupled with ultrasonic dental scaling

Complete Genome Sequence of Rothia mucilaginosa Strain NUM-Rm6536, Isolated from a Human Oral Cavity

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