Implications of adipose-derived stromal cells in a 3D culture system for osteogenic differentiation: an in vitro and in vivo investigation

Shen, Francis H.; Werner, Brian C.; Liang, Haixiang; Shang, Hulan; Yang, Ning; Li, Xudong; Shimer, Adam L.; Balian, Gary; Katz, Adam J.

doi:10.1016/j.spinee.2013.01.002

Cited by 42 publications

(33 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Calcium accumulation during osteogenic differentiation was already described in several studies on adipose-derived stem cells cultured in monolayer2448 or in 3D cultures4950. To the best of our knowledge, this reported study demonstrates for the first time, osteogenic differentiation, including calcification of 3D fat tissue cultures of human origin in vitro .…”

Section: Discussionsupporting

confidence: 70%

“…The presented data support the hypothesis, that osteogenesis can be induced in human fat tissue without isolation of progenitor cells. The presence of mesenchymal stem cells and their osteogenic potential were confirmed for different types of adipose tissue by numerous studies485051. However, no data was reported so far on fat tissue transdifferentiation in vitro .…”

Section: Discussionmentioning

confidence: 90%

See 1 more Smart Citation

Osseous differentiation of human fat tissue grafts: From tissue engineering to tissue differentiation

Bondarava¹,

Cattaneo²,

Ren³

et al. 2017

Sci Rep

View full text Add to dashboard Cite

Conventional bone tissue engineering approaches require isolation and in vitro propagation of autologous cells, followed by seeding on a variety of scaffolds. Those protracted procedures impede the clinical applications. Here we report the transdifferentiation of human fat tissue fragments retrieved from subcutaneous fat into tissue with bone characteristics in vitro without prior cell isolation and propagation. 3D collagen-I cultures of human fat tissue were cultivated either in growth medium or in osteogenic medium (OM) with or without addition of Bone Morphogenetic Proteins (BMPs) BMP-2, BMP-7 or BMP-9. Ca2+ depositions were observed after two weeks of osteogenic induction which visibly increased when either type of BMP was added. mRNA levels of alkaline phosphatase (ALP) and osteocalcin (OCN) increased when cultured in OM alone but addition of BMP-2, BMP-7 or BMP-9 caused significantly higher expression levels of ALP and OCN. Immunofluorescent staining for OCN, osteopontin and sclerostin supported the observed real-time-PCR data. BMP-9 was the most effective osteogenic inducer in this system. Our findings reveal that tissue regeneration can be remarkably simplified by omitting prior cell isolation and propagation, therefore removing significant obstacles on the way to clinical applications of much needed regeneration treatments.

show abstract

Section: Discussionsupporting

confidence: 70%

Section: Discussionmentioning

confidence: 90%

Osseous differentiation of human fat tissue grafts: From tissue engineering to tissue differentiation

Bondarava¹,

Cattaneo²,

Ren³

et al. 2017

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Critical size-calvarial defects are widely employed to study bone healing in animal models, mostly rodents, allowing an easy quantification of the amount of newly formed bone within a bidimensional defect [74, 108–127]. An initial proof of principle of the in vivo osteogenic potential of experimental constructs may be achieved using local intramuscular injection to induce ectopic bone formation [75, 121, 128–133]. Also, segmental defects in long bones of large animals are widely used as clinically relevant models, as resembling the fracture healing process [93, 112, 134–147].…”

Section: Preclinical Evaluation Of Asc Osteoregenerative Potentialmentioning

confidence: 99%

Adipose-Derived Mesenchymal Cells for Bone Regereneration: State of the Art

Barba

Cicione

Bernardini

et al. 2013

BioMed Research International

View full text Add to dashboard Cite

Adipose tissue represents a hot topic in regenerative medicine because of the tissue source abundance, the relatively easy retrieval, and the inherent biological properties of mesenchymal stem cells residing in its stroma. Adipose-derived mesenchymal stem cells (ASCs) are indeed multipotent somatic stem cells exhibiting growth kinetics and plasticity, proved to induce efficient tissue regeneration in several biomedical applications. A defined consensus for their isolation, classification, and characterization has been very recently achieved. In particular, bone tissue reconstruction and regeneration based on ASCs has emerged as a promising approach to restore structure and function of bone compromised by injury or disease. ASCs have been used in combination with osteoinductive biomaterial and/or osteogenic molecules, in either static or dynamic culture systems, to improve bone regeneration in several animal models. To date, few clinical trials on ASC-based bone reconstruction have been concluded and proved effective. The aim of this review is to dissect the state of the art on ASC use in bone regenerative applications in the attempt to provide a comprehensive coverage of the topics, from the basic laboratory to recent clinical applications.

show abstract

“…The ability of 3D culture in paper-based scaffolds to influence the behaviour of EADSC in in vitro culture is likely attributed to changes in the cellular phenotype, particularly morphological changes, with EADSC in 3D culture adopting a spherical morphology compared to the flat and elongated morphology of EADSC in 2D culture. Studies culturing human adipose derived-stem cells in 3D multicellular aggregates have observed similar results in respect to cellular rounding and enhanced osteogenic differentiation (Shen et al 2013), with cell shape previously being demonstrated to influence gene and protein expression in cultured human adipose-derived stem cells (Amos et al 2010). These findings will contribute to the design of more physiologically relevant studies regarding the differentiation capabilities of EADSC, with the potential for use as an efficient and cost-effective method of performing screening assays on cells differentiated in 3D culture.…”

Section: Discussionmentioning

confidence: 66%

A paper-based scaffold for enhanced osteogenic differentiation of equine adipose-derived stem cells

et al. 2015

View full text Add to dashboard Cite

show abstract

Implications of adipose-derived stromal cells in a 3D culture system for osteogenic differentiation: an in vitro and in vivo investigation

Cited by 42 publications

References 30 publications

Osseous differentiation of human fat tissue grafts: From tissue engineering to tissue differentiation

Osseous differentiation of human fat tissue grafts: From tissue engineering to tissue differentiation

Adipose-Derived Mesenchymal Cells for Bone Regereneration: State of the Art

A paper-based scaffold for enhanced osteogenic differentiation of equine adipose-derived stem cells

Contact Info

Product

Resources

About