The therapeutic applications of celery oil seed extract on the plasticizer di(2-ethylhexyl) phthalate toxicity

Abstract

The present study investigated the impact of two doses, 500 mg/kg and 1000 mg/kg, of di(2-ethylhexyl) phthalate (DEHP) and studied the possible therapeutic dose of celery oil seed extract for 6 weeks on some atheroscelerogenic, obesogenic, antioxidant and liver functions in rats. Both doses of DEHP caused over-expression of peroxisome proliferator-activated receptor alpha (PPARα) messenger RNA with significant increase in liver weights, relative liver weights, serum cholesterol (Chol), triglycerides, low-density lipoprotein Chol, liver total lipids, along with an increase in the activities of serum aspartate aminotransferase, alanine aminotransferase, serum endothelin 1 and liver tissue thiobarbituric acid reactive substances (TBARS). Additionally, DEHP administration to rats resulted in significant decrease in final body weights, serum total protein, albumin, liver total protein and serum total nitric oxide. Our study confirmed the role of oral combination of Apium graveolens (celery) oil seed extract at small cumulative doses (50 µl/kg for 6 weeks) with DEHP in ameliorating the toxicological effects of DEHP, which was revealed in reducing the expression of PPARα, lipid profile, with restoring liver functions, vascular oxidative stress and inhibition of TBARS activity.

Antischistosomal and anti-inflammatory activity of garlic and allicin compared with that of praziquantel in vivo

“…After liver damage, hepatic stellate cells (HSCs) undergo a complex transformation or activation process in which the cells change from a quiescent, vitamin A-storing, cell type into activated myofibroblasts. This transformation results in considerable changes including the altered appearance of the cytoskeletal protein smooth muscle α-actin (α-SMA), loss of cellular vitamin A stores [ 41 ], and upregulation of type I and III collagen genes. These pathogenic changes cause excessive deposition of ECM proteins including three large families of glycoproteins, collagens, proteoglycans [ 42 ], and fibronectin [ 43 ] that disrupt the balance of ECM integrity and thereby induce hepatic fibrosis.…”

The effects of di(2‐ethylhexyl)phthalate on rat liver in relation to selenium status

“…On the other hand, there are limited number of studies in literature determining the protective effect of other antioxidants against the hepatotoxicity of DEHP. In a study by El‐Shinnawy (), the researcher showed that Apium graveolens (celery) oil seed extract was protective against the oxidative stress‐producing potential of DEHP in rat liver. However, resveratrol and vitamin C did not provide any protection against the hepatoxicity of DEHP in rats (Botelho et al .…”

Di(2-ethylhexyl)phthalate induces reproductive toxicity via JAZF1/TR4 pathway and oxidative stress in pubertal male rats

“…Throughout the experiment, the rats were allowed free access to food and water in a 12-h light/dark cycle, controlled temperature (20 + 2 C) and humidity (45 + 5%) environment. The DEHP dosage and exposure time in the present study followed previous research (El-Shinnawy, 2015;Madkour, 2014). After 30-day exposure, the rats were sacrificed, and the testes were immediately removed and weighed.…”