Plasma Protein Hydroperoxides During Aging in Humans: Correlation with Paraoxonase 1 (PON1) Arylesterase Activity and Plasma Total Thiols

Mehdi, Mohammad Murtaza; Rizvi, Syed Ibrahim

doi:10.1016/j.arcmed.2013.01.003

Cited by 14 publications

(4 citation statements)

References 46 publications

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“…An inverse correlation between the increasing age and the total plasma thiols were shown before. 19 In our study, inverse correlation was found between the age and the total plasma thiols (p<0.001, r=0.539) in the control group, but not in the retinal vein occlusion patients (p=0.18) ( Table 3). The native and disulfide ratios were not significantly correlated, this may show that there wasn't remarkable shift to the oxidized disulfide groups.…”

Section: Age (Year)supporting

confidence: 45%

Is There Any Association Between the Retinal Vein Occlusion and the Thiol-Disulfifide Homeostasis Which is an Oxidative Stress Indicator?

Polat¹,

Kurt²,

Kılıç³

et al. 2019

Turkiye Klinikleri J Ophthalmol

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In this study, it is aimed to investigate the dynamic thiol-disulfide homeostasis which is an indicator of oxidative stress in retinal vein occlusion patients. M Ma at te er ri ia al l a an nd d M Me et th ho od ds s: : Thirty nine retinal vein occlusion patients and 38 age-gender matched control subjects were included in the study. Exclusion criteria for retinal vein occlusion group were: ocular history and symptoms exceeding 3 weeks, any other history of ocular disease except the retinal vein occlusion, any previous ocular surgeries or lasers, any previous treatment or intra-vitreal injection for the retinal vein occlusion, any other systemic disease except the arterial hypertension. The control group included patients without any ocular or systemic diseases. Native thiol, total thiol, disulfide levels were studied in the serum samples of all participants and native/total thiol and disulfide/total thiol ratios were calculated as percentage. R Re es su ul lt ts s: : Total thiol, native thiol, disulfide, native/total thiol and disulfide/total thiol ratio were not significantly different between the retinal vein occlusion and the control groups. In the control group, age was inversely correlated with the total thiol level, but not with the other serum parameters. In the retinal vein occlusion group, there was no correlation among the age and the serum parameters but the body mass index (BMI) was inversely correlated with the total thiol level. C Co on nc cl lu us si io on n: : There was not any significant difference between the retinal vein occlusion patients and the control subjects for the thiol-disulfide homeostasis. K Ke ey yw wo or rd ds s: : Retinal vein occlusion; thiol-disulfide homeostasis; thiol; disulfide Ö ÖZ ZE ET T A Am ma aç ç: : Bu çalışmada retinal ven tıkanıklığı ile bir oksidatif stres belirteci olan dinamik tiyol-disülfit dengesi arasındaki ilişkinin araştırılması amaçlanmıştır. G Ge er re eç ç v ve e Y Yö ön nt te em ml le er r: : Otuz dokuz retinal ven tıkanıklığı hastası ve 38 yaş ve cinsiyet eşleştirilmiş kontrol birey olmak üzere toplam 77 katılımcı çalışmaya alınmıştır. Retinal ven tıkanıklığı grubu için çalışmadan dışlanma kriterleri: 3 haftayı aşan göz şikayet ve bulguları, retinal ven tıkanıklığı dışında başka oküler hastalık öyküsünün olması, daha önce oküler cerrahi ve lazer öyküsünün olması, retinal ven tıkanıklığı için daha önce intravitreal enjeksiyon dahil olmak üzere herhangi bir tedavi uygulanmış olması, arteryel hipertansiyon dışında sistemik hastalık öyküsünün olması olarak belirlenmiştir. Kontrol grubu sistemik ve oküler hastalığı olmayan katılımcılardı. Native tiyol, total thiol, disülfit düzeyleri katılımcıların kan serumu örneklerinde çalışılarak belirlenmiştir ve native/total tiyol ve disülfit/total tiyol oranları hesaplanarak yüzde olarak kaydedilmiştir. B Bu ul lg gu u-l la ar r: : Total tiyol, native tiyol, disülfit, native/total tiyol oranı ve disülfit/total tiyol oranları retinal ven tıkanıklığı ve kontrol grubu arasında karşılaştırıldığında anlamlı fark bulunmamış...

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Section: Age (Year)supporting

confidence: 45%

Is There Any Association Between the Retinal Vein Occlusion and the Thiol-Disulfifide Homeostasis Which is an Oxidative Stress Indicator?

Polat¹,

Kurt²,

Kılıç³

et al. 2019

Turkiye Klinikleri J Ophthalmol

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“…Oxidative stress and antioxidants have been in the focus of research for decades due to their association with numerous age-related diseases [ 1 – 4 ]. In humans, elevated levels of oxidative stress have been reported in several medical conditions, including neurodegenerative diseases [ 2 ], obesity, diabetes mellitus, and the aging process itself [ 3 – 5 ]. There is some epidemiological evidence on the role of oxidative stress in aging, age-related diseases, and mortality [ 6 – 10 ].…”

Section: Introductionmentioning

confidence: 99%

Associations between Specific Redox Biomarkers and Age in a Large European Cohort: The MARK‐AGE Project

Weber

Stuetz

Toussaint

et al. 2017

Oxidative Medicine and Cellular Longevity

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Oxidative stress and antioxidants play a role in age-related diseases and in the aging process. We here present data on protein carbonyls, 3-nitrotyrosine, malondialdehyde, and cellular and plasma antioxidants (glutathione, cysteine, ascorbic acid, uric acid, α-tocopherol, and lycopene) and their relation with age in the European multicenter study MARK-AGE. To avoid confounding, only data from countries which recruited subjects from all three study groups (five of eight centers) and only participants aged ≥55 years were selected resulting in data from 1559 participants. These included subjects from (1) the general population, (2) members from long-living families, and (3) their spouses. In addition, 683 middle-aged reference participants (35–54 years) served as a control. After adjustment for age, BMI, smoking status, gender, and country, there were differences in protein carbonyls, malondialdehyde, 3-nitrotyrosine, α-tocopherol, cysteine, and glutathione between the 3 study groups. Protein carbonyls and 3-nitrotyrosine as well as cysteine, uric acid, and lycopene were identified as independent biomarkers with the highest correlation with age. Interestingly, from all antioxidants measured, only lycopene was lower in all aged groups and from the oxidative stress biomarkers, only 3-nitrotyrosine was increased in the descendants from long-living families compared to the middle-aged control group. We conclude that both lifestyle and genetics may be important contributors to redox biomarkers in an aging population.

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“…A closer analysis (a gender- and age-adjusted multivariate test) revealed that both PON1–Q192R variant and PON1 activity significantly influence FRAP but not total thiol. According to several studies, 33–36 oxidative stress parameters including FRAP and total thiol are affected by age and gender. Therefore, age- and gender-adjusted statistical tests should be used to evaluate the effect of a factor on these parameters.…”

Section: Discussionmentioning

confidence: 99%

The common variant Q192R at the paraoxonase 1 (PON1) gene and its activity are responsible for a portion of the altered antioxidant status in type 2 diabetes

Zargari

Sharafeddin

Mahrooz

et al. 2016

Exp Biol Med (Maywood)

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In this study, we investigated the effects of paraoxonase 1 (PON1) activities and the variant PON1-Q192R on the ferric reducing ability of plasma (FRAP) and total thiol. In addition, we examined the distribution of genotypes of this variant and the relationship of the genotypes with age in patients with type 2 diabetes (T2D). A total of 115 patients with T2D were enrolled in this study. Paraoxonase activity (PON-para) and arylesterase activity (PON-aryl) were determined using spectrophotometric assays. The distribution of the Q192R genotypes was determined by the double substrate method. The antioxidant status was evaluated by determining FRAP and total thiol. The frequencies of Q and R allozyme were 0.78 and 0.22, respectively. The multivariate analysis identified a significant association between the variables PON1-Q192R (Wilks' λ = 0.85, P = 0.002) and PON-aryl (Wilks' λ = 0.896, P = 0.017), with FRAP and total thiol. The significant difference observed for PON1-Q192R and PON-aryl is primarily due to the changes in FRAP levels (η(2 )= 0.127, P = 0.002 for PON1-Q192R; η(2 )= 0.083, P = 0.011 for PON-aryl). The interaction PON1-Q192R-PON-aryl increased the effect sizes from 8 to 19% for FRAP. Only in R-carrying genotypes, there were significant correlations between both PON-para/HDL (r = -0.574, P < 0.001) and PON-aryl/HDL (r = -0.577, P < 0.001) with age. Our data suggest that the variant PON1-Q192R and PON1 activity, particularly PON-aryl, influenced the antioxidant status in T2D. The interaction of this variant and PON1 activity increased the effect size on the antioxidant capacity. Moreover, the presence of the R allozyme may potentiate the effects of age on susceptibility to cardiovascular diseases in T2D.

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Plasma Protein Hydroperoxides During Aging in Humans: Correlation with Paraoxonase 1 (PON1) Arylesterase Activity and Plasma Total Thiols

Cited by 14 publications

References 46 publications

Is There Any Association Between the Retinal Vein Occlusion and the Thiol-Disulfifide Homeostasis Which is an Oxidative Stress Indicator?

Is There Any Association Between the Retinal Vein Occlusion and the Thiol-Disulfifide Homeostasis Which is an Oxidative Stress Indicator?

Associations between Specific Redox Biomarkers and Age in a Large European Cohort: The MARK‐AGE Project

The common variant Q192R at the paraoxonase 1 (PON1) gene and its activity are responsible for a portion of the altered antioxidant status in type 2 diabetes

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