Analysis of Resistant Starches in Rat Cecal Contents Using Fourier Transform Infrared Photoacoustic Spectroscopy

Anderson, Timothy J.; Ai, Yongfeng; Jones, Roger; Houk, R. S.; Jane, Jay‐lin; Zhao, Yan; Birt, Diane F.; McClelland, John F.

doi:10.1021/jf3042616

Cited by 11 publications

(7 citation statements)

References 32 publications

(48 reference statements)

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“…The results reported here have also been discussed in our previous publication (57). Our principal goal in this work is to develop FTIR-PAS as a practical alternative to the enzymatic assay normally used to measure starch content and demonstrate its use for multiple forms of RS.…”

supporting

confidence: 62%

“…The ability of FTIR-PAS to detect and measure so many different factors in such a wide variety of biological materials makes it a natural choice for analysis of digesta and fecal material in dietary and disease studies, but our recent work reported here and elsewhere (57,58) appears to be the first such uses. The results reported here have also been discussed in our previous publication (57).…”

mentioning

confidence: 99%

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Measurement of Resistant Starches in Rat Cecal Contents using Fourier Transform Infrared Photoacoustic Spectroscopy

Anderson

Jones

et al. 2014

ACS Symposium Series

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supporting

confidence: 62%

mentioning

confidence: 99%

Measurement of Resistant Starches in Rat Cecal Contents using Fourier Transform Infrared Photoacoustic Spectroscopy

Anderson

Jones

et al. 2014

ACS Symposium Series

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“…It was first used by Dempster (51) and was further developed by Nier (52). Currently, EI is the most commonly used ion source utilized with GC-MS instruments.…”

Section: Electron Ionizationmentioning

confidence: 99%

Mass Spectrometry and Fourier Transform Infrared Spectroscopy for Analysis of Biological Materials

Anderson

2014

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“…In a companion study to this one that used the same samples, Anderson et al [52] determined through enzymatic assay that the average cecal starch content (dsb) for the control, HA7, OS-HA7, and StA-HA7 diets were 0.7, 18.3, 48.8, and 21.9 %, respectively. The residual starch differences are most likely attributed to the ability of each starch to be fermented in the cecum.…”

Section: Mass Spectrometrymentioning

confidence: 99%

High-resolution time-of-flight mass spectrometry fingerprinting of metabolites from cecum and distal colon contents of rats fed resistant starch

Anderson

Jones

et al. 2013

Anal Bioanal Chem

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Time-of-flight mass spectrometry along with statistical analysis was utilized to study metabolic profiles among rats fed resistant starch (RS) diets. Fischer 344 rats were fed four starch diets consisting of 55 % (w/w, dbs) starch. A control starch diet consisting of corn starch was compared against three RS diets. The RS diets were high-amylose corn starch (HA7), HA7 chemically modified with octenyl succinic anhydride, and stearic-acidcomplexed HA7 starch. A subgroup received antibiotic treatment to determine if perturbations in the gut microbiome were long lasting. A second subgroup was treated with azoxymethane (AOM), a carcinogen. At the end of the 8-week study, cecal and distal colon content samples were collected from the sacrificed rats. Metabolites were extracted from cecal and distal colon samples into acetonitrile. The extracts were then analyzed on an accurate-mass time-of-flight mass spectrometer to obtain their metabolic profile. The data were analyzed using partial least-squares discriminant analysis (PLS-DA). The PLS-DA analysis utilized a training set and verification set to classify samples within diet and treatment groups. PLS-DA could reliably differentiate the diet treatments for both cecal and distal colon samples. The PLS-DA analyses of the antibiotic and no antibiotic-treated subgroups were well classified for cecal samples and modestly separated for distal colon samples. PLS-DA analysis had limited success separating distal colon samples for rats given AOM from those not treated; the cecal samples from AOM had very poor classification. Mass spectrometry profiling coupled with PLS-DA can readily classify metabolite differences among rats given RS diets. Abstract: Time-of-flight mass spectrometry along with statistical analysis was utilized to study metabolic profiles among rats fed resistant starch (RS) diets. Fischer 344 rats were fed four starch diets consisting of 55% (w/w, dsb) starch. A control starch diet consisting of corn starch was compared against three RS diets. The RS diets were high-amylose corn starch (HA7), HA7 chemically modified with octenyl succinic anhydride, and stearic-acid-complexed HA7 starch. A subgroup received antibiotic treatment to determine if perturbations in the gut microbiome were long lasting. A second subgroup was treated with azoxymethane (AOM), a carcinogen. At the end of the 8-week study, cecal and distal colon contents samples were collected from the sacrificed rats. Metabolites were extracted from cecal and distal colon samples into acetonitrile. The extracts were then analyzed on an accurate-mass time-of-flight mass spectrometer to obtain their metabolic profile. The data were analyzed using partial least-squares discriminant analysis (PLS-DA). The PLS-DA analysis utilized a training set and verification set to classify samples within diet and treatment groups. PLS-DA could reliably differentiate the diet treatments for both cecal and distal colon samples. The PLS-DA analyses of the antibiotic and no antibiotictreated subgroups were well cla...

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Analysis of Resistant Starches in Rat Cecal Contents Using Fourier Transform Infrared Photoacoustic Spectroscopy

Cited by 11 publications

References 32 publications

Measurement of Resistant Starches in Rat Cecal Contents using Fourier Transform Infrared Photoacoustic Spectroscopy

Measurement of Resistant Starches in Rat Cecal Contents using Fourier Transform Infrared Photoacoustic Spectroscopy

Mass Spectrometry and Fourier Transform Infrared Spectroscopy for Analysis of Biological Materials

High-resolution time-of-flight mass spectrometry fingerprinting of metabolites from cecum and distal colon contents of rats fed resistant starch

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