Serum albumin enhances bone healing in a nonunion femoral defect model in rats: a computer tomography micromorphometry study

Skaliczki, Gábor; Schandl, Károly; Weszl, Miklós; Major, Tibor; Kovács, Miklós; Skaliczki, J.; Szabó, Miklós; Dobó‐Nagy, Csaba; Lacza, Zsombor

doi:10.1007/s00264-012-1770-8

Cited by 37 publications

(33 citation statements)

References 25 publications

(32 reference statements)

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“…This supports the concentration‐dependent characteristics of albumin described by Bernards, since the adsorbed amount of serum albumin was potentially higher after freeze‐drying compared to soaking . Additionally, these albumin‐coated mineralized allografts successfully bridged non‐union femoral defects and increased trabecular thickness in rats, while uncoated materials failed . This animal study has recently been confirmed in humans.…”

Section: Local Albumin Administrationsupporting

confidence: 82%

Serum albumin as a local therapeutic agent in cell therapy and tissue engineering

et al. 2016

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Albumin is a major plasma protein that has become ubiquitous in regenerative medicine research. As such, many studies have examined its structure and advantageous properties. However, a systematic and comprehensive understanding of albumin's role, capabilities and therapeutic potential still eludes the field. In the present work, we review how albumin is applied in tissue engineering, including cell culture and storage, in vitro fertilization and transplantation. Furthermore, we discuss how albumin's physiological role extends beyond a carrier for metal ions, fatty acids, pharmacons and growth factors. Albumin acts as a bacteriostatic coating that simultaneously promotes attachment and proliferation of eukaryotic cells. These properties with the combination of free radical scavenging, neutrophil activation and as a buffer molecule already make the albumin protein beneficial in healing processes supporting functional tissue remodeling. Nevertheless, recent data revealed that albumin can be synthesized by osteoblasts and its local concentration is raised after bone trauma. Interestingly, by increasing the local albumin concentration in vivo, faster bone healing is achieved, possibly because albumin recruits endogenous stem cells and promotes the growth of new bone. These data also suggest an active role of albumin, even though a specific receptor has not yet been identified. Together, this discussion sheds light on why the extravascular use of the albumin molecule is in the scope of scientific investigations and why it should be considered as a local therapeutic agent in regenerative medicine. © 2016 BioFactors, 43(3):315-330, 2017.

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Section: Local Albumin Administrationsupporting

confidence: 82%

Serum albumin as a local therapeutic agent in cell therapy and tissue engineering

et al. 2016

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“…In previous in vitro experiments we showed that freeze‐dried serum albumin coating on human allografts provides a convenient milieu for mesenchymal stem cell (MSC) proliferation . Albumin coated human allografts were also implanted in a rat nonunion femur model in vivo , where we found significant defect consolidation at four weeks after implantation . At this time point, albumin coated allografts successfully bridged nonunion bone defects, while uncoated grafts failed.…”

Section: Introductionmentioning

confidence: 74%

“…9 Albumin coated human allografts were also implanted in a rat nonunion femur model in vivo, where we found significant defect consolidation at four weeks after implantation. 10 At this time point, albumin coated allografts successfully bridged nonunion bone defects, while uncoated grafts failed. Later, we investigated the safety and surgical applicability of albumin-coated allografts in a human experiment, during which albumincoated allografts were implanted in 10 cases of aseptic revision arthroplasty as a support for the metal prosthesis.…”

Section: Introductionmentioning

confidence: 93%

Serum albumin coating of demineralized bone matrix results in stronger new bone formation

et al. 2015

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Blood serum fractions are hotly debated adjuvants in bone replacement therapies. In the present experiment, we coated demineralized bone matrices (DBM) with serum albumin and investigated stem cell attachment in vitro and bone formation in a rat calvaria defect model. In the in vitro experiments, we observed that significantly more cells adhere to the serum albumin coated DBMs at every time point. In vivo bone formation with albumin coated and uncoated DBM was monitored biweekly by computed tomography until 11 weeks postoperatively while empty defects served as controls. By the seventh week, the bone defect in the albumin group was almost completely closed (remaining defect 3.0 ± 2.3%), while uncoated DBM and unfilled control groups still had significant defects (uncoated: 40.2 ± 9.1%, control: 52.4 ± 8.9%). Higher density values were also observed in the albumin coated DBM group. In addition, the serum albumin enhanced group showed significantly higher volume of newly formed bone in the microCT analysis and produced significantly higher breaking force and stiffness compared to the uncoated grafts (peak breaking force: uncoated: 15.7 ± 4 N, albumin 46.1 ± 11 N). In conclusion, this investigation shows that implanting serum albumin coated DBM significantly reduces healing period in nonhealing defects and results in mechanically stronger bone. These results also support the idea that serum albumin coating provides a convenient milieu for stem cell function, and a much improved bone grafting success can be achieved without the use of exogenous stem cells.

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“…Serum albumin‐coated bone allograft (BoneAlbumin) has been intensively investigated lately, with outcomes data demonstrating an ability of BoneAlbumin to facilitate new bone formation in non‐union and critical size defect models in rats (Horvathy, Vacz, et al, ; Skaliczki et al, ; Weszl et al, ). BoneAlbumin was also shown to decrease donor site morbidity and enhance bone formation after anterior cruciate ligament reconstruction with bone‐patellar tendon‐bone autografts (Schandl et al, ).…”

Section: Introductionmentioning

confidence: 99%

Serum albumin‐coated bone allograft (BoneAlbumin) results in faster bone formation and mechanically stronger bone in aging rats

Horváthy

Schandl

Schwarz

et al. 2019

J Tissue Eng Regen Med

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Serum albumin‐coated bone allografts (BoneAlbumin) have successfully supported bone regeneration in various experimental models by activating endogenous progenitors. However, the effect of tissue aging, linked to declining stem cell function, has yet to be explicitly examined within the context of BoneAlbumin's regenerative capacity. Stem cell function was tested with an in vitro attachment assay, which showed that albumin coating increases stem cell attachment on demineralized bone surfaces in an aging cell population. Bone regeneration was investigated in vivo by creating critical size bone defects on the parietal bones of aging female rats. Demineralized bone matrices with and without serum albumin coating were used to fill the defects. Bone regeneration was determined by measuring the density and the size of the remaining bone defect with computed tomography (CT). Microcomputed tomography (MicroCT) and mechanical testing were performed on the parietal bone explants. In vivo CT and ex vivo microCT measurements showed better regeneration with albumin‐coated grafts. Additionally, the albumin‐coated group showed a twofold increase in peak fracture force compared with uncoated allografts. In the present study, serum albumin‐coated demineralized bone matrices successfully supported faster and functionally superior bone regeneration in aging rats. Because stem cell function, a key contributor of bone remodelling, decreases with age and serum albumin is an effective activator of endogenous progenitor cells, this method could be an effective and safe adjuvant in bone regeneration of aging adult and osteo‐compromised populations.

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Serum albumin enhances bone healing in a nonunion femoral defect model in rats: a computer tomography micromorphometry study

Abstract: Our results showed that serum albumin coating of bone grafts can enhance the remodelling and efficacy of treatment in a nonunion model.

Cited by 37 publications

References 25 publications

Serum albumin as a local therapeutic agent in cell therapy and tissue engineering

Serum albumin as a local therapeutic agent in cell therapy and tissue engineering

Serum albumin coating of demineralized bone matrix results in stronger new bone formation

Serum albumin‐coated bone allograft (BoneAlbumin) results in faster bone formation and mechanically stronger bone in aging rats

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