Ultra high performance liquid chromatography tandem mass spectrometry determination and profiling of prohibited steroids in human biological matrices. A review

Gosetti, Fabio; Mazzucco, Eleonora; Gennaro, Maria Carla; Marengo, Emílio

doi:10.1016/j.jchromb.2012.12.003

Cited by 112 publications

(76 citation statements)

References 130 publications

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“…In the past decade, column chemistries, column stability, and column resolution have improved to the point where this method was revisited with more modern technologies (15). Neither hypercarb nor zirconium columns were reassessed due to the lack of their availability in a UHPLC format.…”

Section: Resultsmentioning

confidence: 99%

Marketplace Analysis of Conjugated Estrogens: Determining the Consistently Present Steroidal Content with LC-MS

Levy

Boyne

Rogstad

et al. 2015

AAPS J

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Abstract. Conjugated estrogens purified from pregnant mares urine has been used as estrogen hormone replacement therapy since 1942. Previously, methods were proposed to identify and quantify the components of this complex mixture but ultimately were withdrawn due to incomplete characterization of the product and difficulties in transferring the method between laboratories. The aim of the current study is to develop a LC method that can reliably detect multiple steroidal components in conjugated estrogen tablets and measure their relative amount. The method developed was optimized for UHPLC columns, and the elution profile was analyzed using high-resolution mass spectrometry. A total of 60 steroidal components were identified using their exact m/z, product ion spectra of known, and predicted conjugated estrogen structures. These components were consistently present in 23 lots of Premarin tablets spanning two production years. The ten conjugated estrogens identified in the USP monograph and other additional estrogens reported elsewhere are among the 60 steroidal components reported here. The LC-MS method was tested in different laboratories using multiple samples, and the obtained results were reproducible among laboratories.

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Section: Resultsmentioning

confidence: 99%

Marketplace Analysis of Conjugated Estrogens: Determining the Consistently Present Steroidal Content with LC-MS

Levy

Boyne

Rogstad

et al. 2015

AAPS J

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“…1) may be taken to attenuate oestrogenic effects, and androgenic herbal products taken to diminish the low period between cycles. [3,4] However, two wellconducted clinical studies have not shown DHEA, DIONE or DIOL to produce any advantageous anabolic or ergogenic effects at all, but have confirmed the risk of adverse effects. In particular, in one of the studies, LDL-cholesterol/HDL-cholesterol ratios were increased by 11%, significantly elevating the risk for cardiovascular disease and reducing luteinising hormone levels, which may decrease testicular and adrenal testosterone production.…”

Section: Prohormonesmentioning

confidence: 99%

“…[4] Over many years, gas chromatography (GC) has proven itself in laboratories to be a useful technique for separating and identifying individual components in mixtures of chemical compounds on various chromatographic columns in terms of their retention characteristics (relative to an internal standard compound), as well as for quantifying them. A variety of detectors, e.g.…”

Section: Identification and Determination Of Prohibited Substancesmentioning

confidence: 99%

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Dietary supplements containing prohibited substances: A review (Part 2)

Bijl¹

2014

S Afr J SM

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REVIEWThe role of prohormones, 'classic' and 'designer' steroids, clenbuterol, peptide hormones and newer molecules causing concern in dietary supplements is discussed. Apart from their potential adverse effects on athletes' health, their non-achievement of increased strength and muscle size, trace quantities present in contaminated dietary supplements can lead to failed doping tests. The methodologies used for the identification and determination of prohibited substances in very low concentrations, mainly liquid chromatography and mass spectrometry, are also addressed. Of concern is the anticipation that the number of dietary supplements containing (not yet) prohibited designer steroids and other performance-enhancing newer chemical entities will increase. Athletes, coaches and sports doctors should therefore be provided with information regarding dietary supplements and be advised to minimise risks for non-intentional ingestion of forbidden substances by using safe products listed on databases, such as those obtainable in The Netherlands and Germany.

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“…In certain studies, liquid chromatography (LC) coupled with MS/MS was used as the primary method for identifying and quantifying the tissue distribution of parent compounds (21,22). Compared with gas chromatography and high-performance liquid chromatography (HPLC), the LC-MS/MS method has a number of advantages, including rapid analytical run-time, minimization of the mobile phase and use of small sample volumes (23,24).…”

Section: Introductionmentioning

confidence: 99%

High performance liquid chromatography: Tandem mass spectrometric determination of cisplatin levels in different visceral pleura layers of rats

Xia

Zhang

et al. 2015

Oncology Letters

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Abstract. The aim of the present study was to investigate the concentration of cisplatin in different layers of the visceral pleura in rats, following drug administration. In this study, a sensitive and specific liquid chromatography method coupled with electrospray ionization-tandem mass spectrometry was established to investigate the disposition of cisplatin in different layers of the visceral pleura in rats. Methodological data, including specificity, linearity, accuracy, recovery, precision and lower limits of quantification, confirmed that this novel method may be used to efficiently quantify the cisplatin concentrations in visceral pleura of rats following administration of the drug. Furthermore, the results demonstrated that the desired drug concentration was not achieved in the outer or inner elastic layers of the visceral pleura following injection with cisplatin through various administration methods.

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Ultra high performance liquid chromatography tandem mass spectrometry determination and profiling of prohibited steroids in human biological matrices. A review

Cited by 112 publications

References 130 publications

Marketplace Analysis of Conjugated Estrogens: Determining the Consistently Present Steroidal Content with LC-MS

Marketplace Analysis of Conjugated Estrogens: Determining the Consistently Present Steroidal Content with LC-MS

Dietary supplements containing prohibited substances: A review (Part 2)

High performance liquid chromatography: Tandem mass spectrometric determination of cisplatin levels in different visceral pleura layers of rats

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