Spatial and temporal changes of subchondral bone proceed to microscopic articular cartilage degeneration in guinea pigs with spontaneous osteoarthritis

Wang, T.; Wen, Chunyi; Yan, Chun-Hoi; Lu, William W.; Chiu, K. Y.

doi:10.1016/j.joca.2013.01.002

Cited by 70 publications

(75 citation statements)

References 23 publications

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“…Other studies have examined quantitative microCT measurements and histology simultaneously in guinea pigs(17–19,23), but we aimed to implement a translational approach to disease assessment. To this end, we have demonstrated that our grading scheme is a useful tool that provides important and complementary data to existing outcome measures in the evaluation of OA.…”

Section: Discussionmentioning

confidence: 99%

“…Thresholds were determined optically using the edge detection method(22). For analysis of tibial subchondral trabecular bone, a region with an area of 1.04 × 1.04 × 0.52 mm 3 was selected as the region of interest (ROI), as previously described (17,23). For analysis of femoral subchondral trabecular bone, a cuboid region of trabecular bone with size of 1.5 × 1.5 × 0.5 mm 3 was identified, as published (23).…”

Section: Methodsmentioning

confidence: 99%

“…MicroCT, a form of advanced radiography, provides high resolution images and the ability to view both 2D and 3D reconstructions of bony structures. It is primarily used in research settings to quantitatively evaluate bony features such as subchondral bone sclerosis, subchondral bone volume fraction (BV/TV), trabecular thickness, and bone tissue mineral density (TMD), particularly in rodent models such as guinea pigs (17–19). Similar to traditional radiography, microCT can be performed longitudinally in vivo , allowing researchers to follow OA progression in live animals and direct appropriate harvest time points in studies(20,21).…”

Section: Introductionmentioning

confidence: 99%

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Development of a microcomputed tomography scoring system to characterize disease progression in the Hartley guinea pig model of spontaneous osteoarthritis

Radakovich

Marolf

Shannon

et al. 2017

Connective Tissue Research

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Aim: There is potential discrepancy between human and laboratory animal studies of osteoarthritis (OA), as radiographic assessment is the hallmark of the former and histopathology the standard for the latter. This suggests a need to evaluate OA in animal models in a manner similar to that utilized in people. Our study aimed to develop a whole joint grading scheme for microcomputed tomography (microCT) images in Hartley guinea pigs, a strain that recapitulates joint changes highlighted in human spontaneous OA. Materials and Methods: Knees from animals aged 2, 3, 5, 9, and 15 months were evaluated via whole joint microCT and standard histologic scoring. Quantitative microCT parameters, such as bone volume/total volume were also collected. Results: Both whole joint microCT and histologic scores increased with advancing age and showed strong correlation (r = 0.89. P < 0.0001). Histologic scores, which focus on cartilage changes, increased progressively with age. Whole joint microCT scores, which characterize bony changes, followed a stepwise pattern: scores increased between 3 and 5 months of age, stayed consistent between 5 and 9 months, and worsened again between 9 and 15 months. Conclusions: This work provides data that advocates the use of a whole joint microCT scoring system in guinea pig studies of OA, as it provides important information regarding bony changes that occur at a different rate than articular cartilage changes. This grading scheme, in conjunction with histology and quantitative microCT measurements, may enhance the translational value of this animal model as it pertains to human work.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Development of a microcomputed tomography scoring system to characterize disease progression in the Hartley guinea pig model of spontaneous osteoarthritis

Radakovich

Marolf

Shannon

et al. 2017

Connective Tissue Research

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“…However, it is possible to find previous results comparing volumetric markers in μCT and CBCT 10,11 , but as described before volumetric markers lack the ability to capture the 3D structure of bone trabecula. Preliminary work, using the proposed features to examine muscle texture in MRI 24,25 , has shown that co-occurrence or run-length features detect pathology associated with diseases affecting muscular texture.…”

Section: Discussionmentioning

confidence: 99%

“…Comparisons of μCT (using the Radon transform 8 ) and CBCT (using the Feldkamp algorithm 9 , that introduces an averaging-blur effect to minimize artifacts of cone-beam imaging) have been recently reported for dental implant sites in cadavers 10 and for assessments of bone quality in femurs obtained from animal models 11 . In conclusion, very little work exists in methods that explore the 3D nature of subchondral texture, and all of them use CT as image modality.…”

Section: Introductionmentioning

confidence: 99%

Validation of CBCT for the computation of textural biomarkers

et al. 2015

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Osteoarthritis (OA) is associated with significant pain and 42.6% of patients with TMJ disorders present with evidence of TMJ OA. However, OA diagnosis and treatment remain controversial, since there are no clear symptoms of the disease. The subchondral bone in the TMJ is believed to play a major role in the progression of OA. We hypothesize that the textural imaging biomarkers computed in high resolution Conebeam CT (hr-CBCT) and μCT scans are comparable. The purpose of this study is to test the feasibility of computing textural imaging biomarkers in-vivo using hr-CBCT, compared to those computed in μCT scans as our Gold Standard. Specimens of condylar bones obtained from condylectomies were scanned using μCT and hr-CBCT. Nine different textural imaging biomarkers (four co-occurrence features and five run-length features) from each pair of μCT and hr-CBCT were computed and compared. Pearson correlation coefficients were computed to compare textural biomarkers values of μCT and hr-CBCT. Four of the nine computed textural biomarkers showed a strong positive correlation between biomarkers computed in μCT and hr-CBCT. Higher correlations in Energy and Contrast, and in GLN (grey-level non-uniformity) and RLN (run length non-uniformity) indicate quantitative texture features can be computed reliably in hr-CBCT, when compared with μCT. The textural imaging biomarkers computed in-vivo hr-CBCT have captured the structure, patterns, contrast between neighboring regions and uniformity of healthy and/or pathologic subchondral bone. The ability to quantify bone texture non-invasively now makes it possible to evaluate the progression of subchondral bone alterations, in TMJ OA.

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Pulsed electromagnetic field improves subchondral bone microstructure in knee osteoarthritis rats through a Wnt/β‐catenin signaling‐associated mechanism

Yang

Gao

et al. 2017

Bioelectromagnetics

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Pulsed electromagnetic field (PEMF) is often used for management of osteoarthritis (OA). The aim of the study was to determine whether PEMF can successfully improve subchondral bone microstructure through a Wnt/β-catenin signaling-associated pathway in rats with knee OA induced by low-dose monosodium iodoacetate (MIA). Seventy-two 12-week-old male Sprague-Dawley rats were randomly assigned to three groups: OA (n = 24), PEMF (n = 24), and Control (n = 24). OA was induced (OA and PEMF groups) by injecting 0.2 mg MIA in rats' right knee joint. The control rats received a single sterile saline injection in the right knee. Rats in the PEMF group were exposed to daily 2 h PEMF exposure with 75 Hz, 1.6 mT for 4 weeks. After 4 weeks, micro-computed tomography (micro-CT), real-time PCR, and immunohistochemistry staining were performed. The PEMF group increased bone volume fraction (BV/TV), trabecular thickness (Tb.Th), trabecular number (Tb.N), and suppressed bone surface/bone volume (BS/BV) and trabecular separation (Tb.Sp) levels in micro-CT analysis. Real-time PCR analysis showed that PEMF promoted tibial subchondral bone's gene expressions of Wnt3a, β-catenin, and OPG, but did not alter LRP5 and RANKL mRNA levels. Similar results involved tibial subchondral bone's protein expressions that were observed in immunohistochemistry staining. These results suggest that PEMF preserved the structural integrity of subchondral bone in knee OA rats by promoting the activation of Wnt/β-catenin signaling and OPG/RANKL/RANK signaling. Bioelectromagnetics. 39:89-97, 2018. © 2017 Wiley Periodicals, Inc.

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Spatial and temporal changes of subchondral bone proceed to microscopic articular cartilage degeneration in guinea pigs with spontaneous osteoarthritis

Abstract: Subchondral bone ultrastructure change occurred at early stage of OA ahead of microscopic cartilage degeneration, which may further impair articular cartilage. It was possibly related to elevated level of osteoblast differentiation.

Cited by 70 publications

References 23 publications

Development of a microcomputed tomography scoring system to characterize disease progression in the Hartley guinea pig model of spontaneous osteoarthritis

Development of a microcomputed tomography scoring system to characterize disease progression in the Hartley guinea pig model of spontaneous osteoarthritis

Validation of CBCT for the computation of textural biomarkers

Pulsed electromagnetic field improves subchondral bone microstructure in knee osteoarthritis rats through a Wnt/β‐catenin signaling‐associated mechanism

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