A synonymous mutation in NOD2 gene was significantly associated with non-specific digestive disorder in rabbit

Zhang, Wenxiu; Zhang, Gong-Wei; Peng, Jianping; Zhang, Juanli; Yu, Yang; Lai, Songjia

doi:10.1016/j.gene.2012.12.091

Cited by 9 publications

(11 citation statements)

References 23 publications

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“…And as an important gut-associated lymphoid tissue, RSR plays key roles in the development of an immune response or immune tolerance to the antigens and pathogens in the intestinal tract (Haasz et al, 1974). Recently, several genes have been discovered to be related to the genetic determination of susceptibility to NSDD in rabbit, including MyD88, TLR4, NOD2, NLRP3, NLRP12, TYK2, JAK1 and STAT3 (Zhang et al, 2011;Chen et al, 2013a;Liu et al, 2013;Yang et al, 2013b;Zhang et al, 2013;Fu et al, 2014Fu et al, , 2015. It is also proved that several miRNAs play an important role in the pathogenesis of experimental colitis and human IBD.…”

Section: Discussionmentioning

confidence: 99%

“…It is well known that NSDD could be induced by several environmental factors, such as coccidiosis, bacterial infection, fibre-deficient diet, sanitary status and epizootic rabbit enterocolitis (Marai et al, 2010). Meanwhile, individual genetic variation in susceptibility to NSDD has also been observed in recent years (Bartel, 2004;Garreau et al, 2008;Zhang et al, 2011;Chen et al, 2013a;Liu et al, 2013;Yang et al, 2013b;Zhang et al, 2013).…”

Section: Introductionmentioning

confidence: 88%

“…IBD or NSDD-related 25 genes and 25 miRNAs were retrieved from published papers Feng et al, 2012;Ghorpade et al, 2013;Koukos et al, 2013;Liu et al, 2013;Nata et al, 2013;Yang et al, 2013aYang et al, , 2013b Zhang et al, 2013;Fu et al, 2014;Lu et al, 2014;Carethers et al, 2015;Fu et al, 2015;Kalla et al, 2015), and their expression levels were then detected by RT-qPCR method in ileum, RSR and colon tissues. The relative expression level of the genes and miRNAs are shown in Figure 1 and Figure 2, in which the highest expression one is set to 1.…”

Section: Differentially Expressed Mrnas and Mirnasmentioning

confidence: 99%

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Investigation of significant microRNA-mRNA pairs associated with nonspecific digestive disorder in rabbits

et al. 2017

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Nonspecific digestive disorders (NSDD) are one of the major intestinal problems in rabbit, with considerable economic losses in industrial rabbit farms. MicroRNAs (miRNAs), as small non-coding RNAs, have significant biological involvement in intestinal disorders. In this study, we investigated the expression levels of 25 genes and 25 miRNAs in ileum, rabbit sacculus rotundus (RSR) and colon tissues from 9 rabbits with different severity of NSDD. These molecules have been found to be related to NSDD or inflammatory bowel disease, which will help recognise the miRNA-mRNA pairs. Finally, 108 possible pairs of miRNAmRNA pairs with an anti-correlation were identified by Pearson's correlation analysis between differentially expressed 25 miRNAs and 23 mRNAs. Ninety-five of these miRNA-mRNA pairs were hitherto unexplored, and their roles in NSDD biology require further elucidation. Our results give a clue to the potential miRNAmRNA pairs for the NSDD that can further improve the understanding of the pathogenesis of NSDD in rabbit.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 88%

Section: Differentially Expressed Mrnas and Mirnasmentioning

confidence: 99%

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Investigation of significant microRNA-mRNA pairs associated with nonspecific digestive disorder in rabbits

et al. 2017

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“…Mutations in NOD2 were found in patients with Crohn’s disease, Blau syndrome and in rabbit with NSDD (Hugot et al, 2001; Ogura et al, 2001; Zhang et al, 2013). Excluding NLRP12 and NOD2 , other NLR members also were involved in the complicated signaling pathways mediating the cytokines and adjusting the process of inflammatory diseases.…”

Section: Discussionmentioning

confidence: 99%

Single Nucleotide Polymorphisms of <italic>NLRP12</italic> Gene and Association with Non-specific Digestive Disorder in Rabbit

Liu¹,

Zhang²,

Xiao

et al. 2013

Asian Australas. J. Anim. Sci

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The NLRP12 (NLR family, pyrin domain containing 12) serves as a suppressor factor in the inflammatory response and protects the host against inflammation-induced damage. In the present study, we aimed to study the polymorphisms of NLRP12 gene and its association with susceptibility to non-specific digestive disorder (NSDD) in rabbits. We re-sequenced the entire coding region of the rabbit NLRP12 gene and detected a total of 19 SNPs containing 14 synonymous and five non-synonymous variations. Among them, the coding SNP (c.1682A>G), which would carry a potential functional implication, was subsequently subjected to genotyping for case-control association study (272 cases and 267 controls). The results revealed that allele A was significantly protective against NSDD with an odds ratio value of 0.884 (95% confidence interval, 0.788 to 0.993; p = 0.038). We also experimentally induced NSDD in growing rabbits by feeding a fibre-deficient diet and subsequently investigated NLRP12 mRNA expression. The mRNA expression of NLRP12 in healthy status was significantly higher than that in severe NSDD (p = 0.0016). The highest expression was observed in individuals carrying the protective genotype AA (p = 0.0108). These results suggested that NLRP12 was significantly associated with the NSDD in rabbits. However, the precise molecular mechanism of NLRP12 involving in the development of rabbit NSDD requires further research.

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“…Identifying and understanding the DNA markers associated with the disease is the first step towards applying this information in breeding for disease resistance, which may be an alternative strategy to control this disease. Our recent studies revealed that SNP in pattern recognition receptors (PRR) genes, such as Toll-like receptor 4 (TLR4) and nucleotide-binding oligomerization domain 2 (NOD2) are associated with NSDD in rabbits (Zhang et al, 2011;Zhang et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Dectin-1 gene polymorphism is associated with susceptibility to nonspecific digestive disorders and cytokine expression in rabbits1

Zhang

zhang

Chen

et al. 2013

Journal of Animal Science

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Dectin-1 plays a critical role in the pathogenesis of intestinal inflammation by recognizing the pathogenic agents and mediating cytokine responses. The objective of this study was to establish the association between dectin-1 polymorphisms and susceptibility to nonspecific digestive disorders (NSDD) and cytokine expression in rabbits. A total of 7 coding SNP were detected in dectin-1 gene. The genetic association between SNP (ss707197675A > G) and susceptibility to NSDD was evaluated using a case-control study (178 cases and 174 controls). The results revealed that the A allele was associated with an increased risk of developing NSDD in rabbits. The AA genotype significantly increased the genetic susceptibility to NSDD with odds ratio of 4.76 (95% confidence interval, 1.92-12.50, P = 0.0002) compared with GG and GA genotypes. We also experimentally induced NSDD in another independent growing rabbit population by feeding a low-fiber diet and subsequently investigated the cytokine mRNA expression. Among the 4 studied cytokines, the expression of interferon-γ, IL-17F, and IL-22 were increased 2.8 to 6.0-fold in AA genotype compared with GG genotype (P < 0.01). The greater IL-17F and IL-22 mRNA expressions indicated a positive correlation with severe intestinal inflammation (P < 0.05). The decreased expression of IL-10 was associated with severe intestinal inflammation (P = 0.006), but IL-10 expression was not influenced by dectin-1 genotype. In conclusion, polymorphism ss707197675 of dectin-1 is related with susceptibility to NSDD and increased expression of proinflammatory cytokines, and dectin-1 could be an important candidate gene associated with NSDD in rabbits.

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A synonymous mutation in NOD2 gene was significantly associated with non-specific digestive disorder in rabbit

Cited by 9 publications

References 23 publications

Investigation of significant microRNA-mRNA pairs associated with nonspecific digestive disorder in rabbits

Investigation of significant microRNA-mRNA pairs associated with nonspecific digestive disorder in rabbits

Single Nucleotide Polymorphisms of <italic>NLRP12</italic> Gene and Association with Non-specific Digestive Disorder in Rabbit

Dectin-1 gene polymorphism is associated with susceptibility to nonspecific digestive disorders and cytokine expression in rabbits1

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A synonymous mutation in NOD2 gene was significantly associated with non-specific digestive disorder in rabbit

Cited by 9 publications

References 23 publications

Investigation of significant microRNA-mRNA pairs associated with nonspecific digestive disorder in rabbits

Investigation of significant microRNA-mRNA pairs associated with nonspecific digestive disorder in rabbits

Single Nucleotide Polymorphisms of &lt;italic&gt;NLRP12&lt;/italic&gt; Gene and Association with Non-specific Digestive Disorder in Rabbit

Dectin-1 gene polymorphism is associated with susceptibility to nonspecific digestive disorders and cytokine expression in rabbits1

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Single Nucleotide Polymorphisms of <italic>NLRP12</italic> Gene and Association with Non-specific Digestive Disorder in Rabbit