Dynamized follicle-stimulating hormone affects the development of ovine preantral follicles cultured in vitro

Lima, Laritza Ferreira de; Rocha, Rebeca Magalhães Pedrosa; Alves, Anelise Maria Costa Vasconcelos; Saraiva, M.V.A.; Araújo, Valdevane Rocha; Lima, Isadora Machado Teixeira; Lopes, C.A.P.; Báo, Sônia Nair; Campello, C.C.; Rodrigues, Ana Paula Ribeiro; Figueiredo, J.R.

doi:10.1016/j.homp.2012.11.002

Cited by 15 publications

(23 citation statements)

References 22 publications

(28 reference statements)

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“…In our culture system, reduction in follicular survival was observed in all treatments at the beginning of the culture and it is probably associated with apoptosis as high apoptosis index was observed in the present study. Ovine (Lima et al 2013) and caprine studies (Saraiva et al 2008) have reported such reduction in follicular viability after in vitro culture of pre-antral follicles. On the other hand, it could be inferred that the inhibitory action of AMH on follicular activation is not related to an additional reduction in follicular survival.…”

Section: Discussionmentioning

confidence: 98%

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Immunolocalization of the Anti‐Müllerian Hormone (AMH) in Caprine Follicles and the Effects of AMH on In Vitro Culture of Caprine Pre‐antral Follicles Enclosed in Ovarian Tissue

Rocha

Lima

Carvalho

et al. 2016

Reprod Domestic Animals

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The aims of this study were to evaluate the localization, by immunohistochemistry, of the anti-Müllerian hormone (AMH) in goat ovaries and to investigate its effects on the in vitro survival and development of caprine pre-antral follicles enclosed in fragments of ovarian tissue. Pre-antral follicles were cultured in vitro for 1 or 7 days in α-MEM(+) in the absence or presence of kit ligand (KL; 50 ng/ml, positive control) or AMH (50 or 150 ng/ml). The results showed that AMH was localized in oocytes and granulosa cells from the primordial follicle to antral follicle stages. Addition of AMH maintained the percentage of developing follicles, similar to that in the uncultured control; however, the percentage of developing follicles was significantly lower than that in the cultured control and KL. Nonetheless, addition of AMH to the culture medium did not affect survival rates and follicular growth. In conclusion, this study demonstrated that the expression of AMH varies according to the compartment and stage of follicular development. Furthermore, AMH inhibits the activation of caprine primordial follicles.

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Section: Discussionmentioning

confidence: 98%

“…Ovine (Lima et al. ) and caprine studies (Saraiva et al. ) have reported such reduction in follicular viability after in vitro culture of pre‐antral follicles.…”

Section: Discussionmentioning

confidence: 98%

Immunolocalization of the Anti‐Müllerian Hormone (AMH) in Caprine Follicles and the Effects of AMH on In Vitro Culture of Caprine Pre‐antral Follicles Enclosed in Ovarian Tissue

Rocha

Lima

Carvalho

et al. 2016

Reprod Domestic Animals

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“…Immediately postmortem, ovaries were washed in 70% alcohol followed by two rinses in minimum essential medium (MEM) supplemented with 100 μg/ml penicillin and 100 μg/ml streptomycin plus 25 mM HEPES. Ovaries were transported within 1 hr to the laboratory into tubes containing 15 ml of MEM‐HEPES at 4°C (Lima et al, ) and intended for a two‐step in vitro culture, that is first step (in vitro culture of ovarian tissue) and second step (in vitro culture of secondary isolated follicles), as we can see on Figure .…”

Section: Methodsmentioning

confidence: 99%

Early ovine preantral follicles have a potential to grow until antral stage in two‐step culture system in the presence of aqueous extract of Justicia insularis

Mbemya

Sá

Guerreiro

et al. 2019

Reprod Domestic Animals

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The objective of this study was to determine whether preantral follicles cultured in vitro for 7 days within ovine ovarian cortical strips could be isolated at the secondary follicles (SF) and grown until antral stage during an additional 6 days period of in vitro culture in the presence of aqueous extract of Justicia insularis. Fresh ovarian fragments from 16 adult sheep were fixed for histological analysis (Control 1) or in vitro cultured individually in α‐MEM+ supplemented with 0.3 mg/ml J. insularis (Step 1) for 7 days. Part of the fragments then were fixed for histological analysis (in vitro culture group). Remaining fragments were exposed stepwise to increasing trehalose concentrations before immediate isolation of SF and viability assessment (Control 2) or after 6 days of culture in α‐MEM++ supplemented with 0.3 mg/ml J. insularis (Step 2). In Step 1, percentage of follicular activation was 80%. In Step 2, a significant increase (p < 0.05) in follicular diameter and antrum formation within 6 days in vitro culture of isolated follicles was achieved. The total antioxidant capacity from both steps significantly increase (p < 0.05) from day 2 to day 6. Confocal analysis of oocytes showed 57.14% oocytes with homogeneous distribution and 42.86% with peri‐cortical distribution. In conclusion, SF can be successfully isolated from sheep ovarian cortex after 7 days of culture and are capable of surviving and forming an antral cavity if cultured in vitro for an additional 6 days in the presence of 0.3 mg/ml J. insularis.

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“…Immediately postmortem, ovaries were washed in 70% alcohol followed by two rinses in minimum essential medium (MEM) supplemented with 100 µg/mL penicillin and 100 µg/mL streptomycin plus 25 mM HEPES. Ovaries were transported within 1 h to the laboratory into tubes containing 15 mL of MEM-HEPES at 4 °C [5].…”

Section: Source Of Ovariesmentioning

confidence: 99%

“…Some in vitro studies have demonstrated that the addition of FSH to the culture medium is important to maintain viability and to promote ovine follicular activation and further growth in vitro [3][4][5]. In addition, Magalhães et al [6] showed that the addition of 50 ng/mL recombinant bovine FSH (rFSH) during in vitro culture of goat preantral follicles maintained viability, activation and follicular growth.…”

mentioning

confidence: 99%

Justicia insularis Improves the in vitro Survival and Development of Ovine Preantral Follicles Enclosed in Ovarian Tissue

Mbemya¹,

Guerreiro²,

Donfack³

et al. 2017

JPP

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Objectives: Evaluating the addition effect of J. insularis extract and FSH on the survival, activation and ROS production after in vitro culture of ovine preantral follicles enclosed in ovarian tissue. Methods: In the first experiment, ovarian fragments were fixed (non-cultured control) or in vitro cultured in α-MEM + (cultured control), α-MEM + supplemented with FSH 50 ng/mL, or in α-MEM + supplemented with J. insularis (JUS0.3; 1.25 or 5 mg/mL) for 1 or 7 days, at 39˚C, 5% CO 2 . In the second experiment, fragments were fixed or cultured in α-MEM + supplemented with anethole 300 µg/mL + FSH 50 ng/mL or in α-MEM + supplemented with anethole 300 µg/mL + 0.3 mg/mL JUS. Key findings: JUS0.3 was the only treatment that maintained the percentage of morphologically normal follicles similar to non-cultured control even after 7 days of culture. After 7 days of culture, a higher (p < 0.05) percentage of developing follicles was observed in JUS5 treatment compared with the other treatments except JUS1.25. In the second experiment, FSH maintained the percentage of normal follicles and promoted activation of primordial follicles. A reduction (p < 0.05) of stromal cell density was observed in MEM + +ANE supplemented with JUS or FSH. Conclusions: J. insularis in a concentration-dependent manner maintained the levels of ROS and improved in vitro follicular survival and activation of ovine primordial follicles.

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Dynamized follicle-stimulating hormone affects the development of ovine preantral follicles cultured in vitro

Abstract: FSH 6cH (24 h) treatment maintained the viability, and promoted the activation and in vitro growth of ovine PFs.

Cited by 15 publications

References 22 publications

Immunolocalization of the Anti‐Müllerian Hormone (AMH) in Caprine Follicles and the Effects of AMH on In Vitro Culture of Caprine Pre‐antral Follicles Enclosed in Ovarian Tissue

Immunolocalization of the Anti‐Müllerian Hormone (AMH) in Caprine Follicles and the Effects of AMH on In Vitro Culture of Caprine Pre‐antral Follicles Enclosed in Ovarian Tissue

Early ovine preantral follicles have a potential to grow until antral stage in two‐step culture system in the presence of aqueous extract of Justicia insularis

Justicia insularis Improves the in vitro Survival and Development of Ovine Preantral Follicles Enclosed in Ovarian Tissue

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