MiRNA‐362‐3p induces cell cycle arrest through targeting of E2F1, USF2 and PTPN1 and is associated with recurrence of colorectal cancer

Christensen, Lise Lotte; Tobiasen, Heidi; Holm, Anja; Schepeler, Troels; Ostenfeld, Marie Stampe; Thorsen, Kasper; Rasmussen, Mads Heilskov; Birkenkamp-Demtroeder, Karin; Sieber, Oliver M.; Gibbs, Peter; Lubiński, Jan; Lamy, Philippe; Laurberg, Søren; Øster, Bodil; Hansen, Kristian Q.; Hagemann‐Madsen, Rikke; Byskov, Kristina; Ørntoft, Torben F.; Andersen, Claus L.

doi:10.1002/ijc.28010

Cited by 91 publications

(73 citation statements)

References 43 publications

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“…Expression level of NDRG4 also has been found strongly associated with the prediction and prognosis of colorectal cancer. Evidence suggested E2F1, BMP3 and ABL1 are associated with the progression of CRC, but mostly induced by non-coding RNAs [22][23][24]. These findings further supported the reliability of the prognosis ability of our conducted 9-gene-pair signature.…”

Section: Discussionsupporting

confidence: 79%

Signature consist of nine differentially methylated gene pairs to predict the prognosis for stage II colorectal cancer patients

Zeng¹,

Li²,

Wei³

et al. 2018

Oncotarget

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Colorectal cancer (CRC) is the third most commonly malignance cancer worldwide. The quality of life and life expectancy of CRC survivors are seriously impacted by the recurrence after resection surgery. Therefore, there is a need to develop a reliable signature to predict the recurrence after resection surgery in CRC patients. Epigenetic alterations, especially gene methylation, have been found highly related to the metastasis of tumors. However, limited evidence is available for CRC patients. Moreover, there remains a knowledge gap of a comprehensive view of gene methylation in stage II CRC. In this study, we conducted and validated a differentially methylated 9-gene-pair prognosis signature to predict recurrence after resection surgery in stage II CRC patients. In addition, we use Univariable and Multivariable Cox regression to estimate the association between relapse free survival times in relation to each candidate gene pairs and the 9-gene-pair score. Moreover, the network analysis found 8 hub genes, including NDRG4 and BMP3, out of all candidate genes have large degree of interactions in the protein-protein interaction network. Seven hub genes have been found associated with CRC progression. Furthermore, the function enrichment analysis suggested that differentially methylated genes were mostly enriched in the cell cycle pathway. In summary, a 9-gene-pair signature was developed and validated in this study. The signatures identified in this study have the potential to be applied in the prognosis of post-surgery recurrence in the stage II CRC patents.

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Section: Discussionsupporting

confidence: 79%

Signature consist of nine differentially methylated gene pairs to predict the prognosis for stage II colorectal cancer patients

Zeng¹,

Li²,

Wei³

et al. 2018

Oncotarget

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show abstract

“…Other studies have shown differential expression of miR-362-3p in various tissues including the fetal brain of mice, estrogen receptor-positive breast tumors, gastric cancer, and melanoma as well as downregulation of miR-329 in glioma, neuroblastoma, and the mouse model of Rett syndrome. 20,22,29,[33][34][35][36][37][38] The detailed mechanism, however, that governs the differential expression of the two miRs has not been fully elucidated.…”

mentioning

confidence: 99%

Downregulation of microRNA-362-3p and microRNA-329 promotes tumor progression in human breast cancer

Kang

et al. 2015

Cell Death Differ

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p130Cas regulates cancer progression by driving tyrosine receptor kinase signaling. Tight regulation of p130Cas expression is necessary for survival, apoptosis, and maintenance of cell motility in various cell types. Several studies revealed that transcriptional and post-translational control of p130Cas are important for maintenance of its expression and activity. To explore novel regulatory mechanisms of p130Cas expression, we studied the effect of microRNAs (miRs) on p130Cas expression in human breast cancer MCF7 cells. Here, we provide experimental evidence that miR-362-3p and miR-329 perform a tumor-suppressive function and their expression is downregulated in human breast cancer. miR-362-3p and miR-329 inhibited cellular proliferation, migration, and invasion, thereby suppressing tumor growth, by downregulating p130Cas. Ectopic expression of p130Cas attenuated the inhibitory effects of the two miRs on tumor progression. Relative expression levels of miR-362-3p/329 and p130Cas between normal and breast cancer correlated inversely; miR-362-3p/329 expression was decreased, whereas that of p130Cas increased in breast cancers. Furthermore, we showed that downregulation of miR-362-3p and miR-329 was caused by differential DNA methylation of miR genes. Enhanced DNA methylation (according to methylation-specific PCR) was responsible for downregulation of miR-362-3p and miR-329 in breast cancer. Taken together, these findings point to a novel role for miR-362-3p and miR-329 as tumor suppressors; the miR-362-3p/miR-329-p130Cas axis seemingly has a crucial role in breast cancer progression. Thus, modulation of miR-362-3p/miR-329 may be a novel therapeutic strategy against breast cancer. Cell Death and Differentiation (2016) 23, 484-495; doi:10.1038/cdd.2015; published online 4 September 2015 p130Cas/breast cancer anti-estrogen resistance 1 is a member of the Cas (Crk-associated substrate) family of adaptor proteins and has a central role in tyrosine kinasebased signaling related to cell adhesion, migration, cell cycle control, apoptosis, development, and cancer progression.

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“…At present, miRNAs research provides a new direction for tumor metastasis. Previous studies suggested a possible relationship between gastrointestinal cancers and miR-362-3p overexpression [9,21]. However, the role of miR-362-3p in GC, especially invasion and metastasis, is not well understood.…”

Section: Discussionmentioning

confidence: 95%

Anti-miR-362-3p Inhibits Migration and Invasion of Human Gastric Cancer Cells by Its Target CD82

Yao

et al. 2015

Dig Dis Sci

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MiRNA‐362‐3p induces cell cycle arrest through targeting of E2F1, USF2 and PTPN1 and is associated with recurrence of colorectal cancer

Cited by 91 publications

References 43 publications

Signature consist of nine differentially methylated gene pairs to predict the prognosis for stage II colorectal cancer patients

Signature consist of nine differentially methylated gene pairs to predict the prognosis for stage II colorectal cancer patients

Downregulation of microRNA-362-3p and microRNA-329 promotes tumor progression in human breast cancer

Anti-miR-362-3p Inhibits Migration and Invasion of Human Gastric Cancer Cells by Its Target CD82

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