Characterisation of genes transcriptionally upregulated in the liver of sand goby (Pomatoschistus minutus) by 17α-ethinyloestradiol: Identification of distinct vitellogenin and zona radiata protein transcripts

Humble, Joseph L.; Hands, E.; Saaristo, Minna; Lindström, Kai; Lehtonen, Kari K.; Cerio, Oihane Díaz de; Cancio, Ibon; Wilson, Gemma; Craft, John A.

doi:10.1016/j.chemosphere.2012.11.053

Cited by 15 publications

(10 citation statements)

References 40 publications

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“…Somados ao que foi apresentado no compilado, Scholz e Gutzeit (2000), observaram que machos jovens de Oryzias latipes sofreram reversão sexual e desenvolveram um ovário quando expostos ao estrogênio sintético EE2 (100 ng / L) por 2 meses, e que esta exposição acarretou ainda a diminuição da produção de ovos devido à redução do peso gonadal de fêmeas a 10 e 100 ng / L de EE2, e aromatase foi detectada nos testículos de machos expostos a 10 ng / L EE2. Todos estes comportamentos poderiam estar relacionados a elevação da vitelogenina, uma lipo-fosfo-glicoproteina sintetizada pelo fígado sobre a influência de esteróides sexuais, principalmente o 17-β estradiol e precursoras das proteínas da gema indutível dos ovos em fêmeas adultas, as quais também tendem a sofrerem alterações em organismos expostos a diferentes concentrações de EE2 (Katsiadaki et al, 2010;Kim et al, 2010;Humble et al, 2013).…”

Section: Ciclo De Transformação De Compostos Estrogênicos Esteroidaisunclassified

Riscos ambientais e para saúde humana da liberação de compostos estrogênicos residuais em ambientes aquáticos

Alamino¹,

Silva²

2021

RECURSO ÁGUA - Tecnologias E Pesquisas Para O Uso E a Conservação De Ecossistemas Aquáticos

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Section: Ciclo De Transformação De Compostos Estrogênicos Esteroidaisunclassified

Riscos ambientais e para saúde humana da liberação de compostos estrogênicos residuais em ambientes aquáticos

Alamino¹,

Silva²

2021

RECURSO ÁGUA - Tecnologias E Pesquisas Para O Uso E a Conservação De Ecossistemas Aquáticos

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“…The qPCR reactions were primed with transcript-specific primers. Primers for vtga, vtgb, vtgc and 28S ribosomal RNA (rs28) were presented previously (Humble et al, 2013). Primers for erα and erβ1…”

Section: Qpcr Assaysmentioning

confidence: 99%

“…Recently this situation has been improved when three vtgs transcripts were identified and shown to be inducible by EE2 exposure in male sand goby (Humble et al, 2013). The three vtg complete cDNA sequences have now been fully sequenced (accession AGO64301.1 AGO64302.1 and AGO64303.1).…”

Section: Introductionmentioning

confidence: 99%

Effects of 17α-ethinyl estradiol exposure on estrogen receptors α and β and vitellogenins A, B and C mRNA expression in the liver of sand goby (Pomatoschistus minutus)

Humble

Saaristo

Lindström

et al. 2014

Marine Environmental Research

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This study aims to characterize the estrogen receptor (er) in sand goby (Pomatoschistus minutus) and determine the temporal effects of 17α-ethinyl estradiol (EE2) on erα and vitellogenin (vtg) gene expression in males. Two partial cDNA sequences (erα and erβ1) are presented showing conserved structural features with ers of other species. Transcript levels for both ers were low in control fish but EE2 exposure (11 ng/L, for 29 days) increased both to a pattern similar to vitellogenic females. The relative expression of three vtg genes (vtga, vtgb and vtgc) along with erα was determined in control and male fish exposed to EE2 (11 ng/L) at multiple time-points over 29 days. All four transcripts were significantly induced due to exposure and expression rose during the time course with distinct temporal patterns and vtga reached a substantially higher level at the end of the time course coinciding with rapid elevation in erα expression.

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“…The liver serves an essential and conserved role for metabolic homeostasis in all vertebrates, and reproduction in the case of oviparous species. Several studies have shown that the liver is an important target of endocrine disruption in mammals (Foulds et al, 2017) and fish (De Wit et al, 2008;Feswick, Munkittrick, & Martyniuk, 2017;Humble et al, 2013;Mirbahai et al, 2011). Using a RRBS approach, the present study aimed at characterising the hepatic methylation landscape in adults and investigating the genome-wide changes in DNA methylation in the liver of 168 dph adults that were exposed to EE2 as hatchlings to test the hypothesis of the potential involvement of DNA methylation in the delayed effects of EDCs.…”

Section: Introductionmentioning

confidence: 99%

Genome-wide DNA methylation of the liver reveals delayed effects of early-life exposure to 17-α-ethinylestradiol in the self-fertilizing mangrove rivulus

Voisin

Ulloa

Stockwell

et al. 2020

Preprint

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Organisms exposed to endocrine disruptors in early life can show altered phenotype later in adulthood. Although the mechanisms underlying these long-term effects remain poorly understood, an increasing body of evidence points toward the potential role of epigenetic processes. In the present study, we exposed hatchlings of an isogenic lineage of the self-fertilizing fish mangrove rivulus for 28 days to 4 and 120 ng/L of 17-α-ethinylestradiol. After a recovery period of 140 days, reduced representation bisulfite sequencing (RRBS) was performed on the liver in order to assess the hepatic genome-wide methylation landscape. Across all treatment comparisons, a total of 146 differentially methylated fragments (DMFs) were reported, mostly for the group exposed to 4 ng/L, suggesting a non-monotonic effect of EE2 exposure. Gene ontology analysis revealed networks involved in lipid metabolism, cellular processes, connective tissue function, molecular transport and inflammation. The highest effect was reported for nipped-B-like protein B (NIPBL) promoter region after exposure to 4 ng/L EE2 (+ 21.9%), suggesting that NIPBL could be an important regulator for long-term effects of EE2. Our results also suggest a significant role of DNA methylation in intergenic regions and potentially in transposable elements. These results support the ability of early exposure to endocrine disruptors of inducing epigenetic alterations during adulthood, providing plausible mechanistic explanations for long-term phenotypic alteration. Additionally, this work demonstrates the usefulness of isogenic lineages of the self-fertilizing mangrove rivulus to better understand the biological significance of long-term alterations of DNA methylation by diminishing the confounding factor of genetic variability.

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Characterisation of genes transcriptionally upregulated in the liver of sand goby (Pomatoschistus minutus) by 17α-ethinyloestradiol: Identification of distinct vitellogenin and zona radiata protein transcripts

Cited by 15 publications

References 40 publications

Riscos ambientais e para saúde humana da liberação de compostos estrogênicos residuais em ambientes aquáticos

Riscos ambientais e para saúde humana da liberação de compostos estrogênicos residuais em ambientes aquáticos

Effects of 17α-ethinyl estradiol exposure on estrogen receptors α and β and vitellogenins A, B and C mRNA expression in the liver of sand goby (Pomatoschistus minutus)

Genome-wide DNA methylation of the liver reveals delayed effects of early-life exposure to 17-α-ethinylestradiol in the self-fertilizing mangrove rivulus

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