Fish Oil has Beneficial Effects on Behavior Impairment and Oxidative Stress in Rats Subjected to a Hepatic Encephalopathy Model

Staziaki, Pedro V.; Marques, Camila Moraes; Delattre, A; Cioni, Beatrice; Rufino, Marja; Santos, Fabiola Vila dos; Licks, Franciele; Marroni, Norma Possa; Ferraz, Anete Curte

doi:10.2174/1871527311312010014

Cited by 11 publications

(6 citation statements)

References 57 publications

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“…AA is the main precursor of eicosanoids, which modulate the immune response via a diversity of pathways [ 54 ]. Dietary DHA has the capacity to suppress markers of hepatic damage, hepatic inflammation, oxidative stress and fibrosis in LDLR -/- mouse with induced non-alcoholic steatohepatitis [ 55 ] and is reported to be beneficial in hepatic encephalopathy [ 56 ]. The altered lipid metabolism in elevated levels of DHA in shunt livers compared to healthy control dogs, might therefore be a protective response.…”

Section: Discussionmentioning

confidence: 99%

Aberrant hepatic lipid storage and metabolism in canine portosystemic shunts

et al. 2017

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Non-alcoholic fatty liver disease (NAFLD) is a poorly understood multifactorial pandemic disorder. One of the hallmarks of NAFLD, hepatic steatosis, is a common feature in canine congenital portosystemic shunts. The aim of this study was to gain detailed insight into the pathogenesis of steatosis in this large animal model. Hepatic lipid accumulation, gene-expression analysis and HPLC-MS of neutral lipids and phospholipids in extrahepatic (EHPSS) and intrahepatic portosystemic shunts (IHPSS) was compared to healthy control dogs. Liver organoids of diseased dogs and healthy control dogs were incubated with palmitic- and oleic-acid, and lipid accumulation was quantified using LD540. In histological slides of shunt livers, a 12-fold increase of lipid content was detected compared to the control dogs (EHPSS P<0.01; IHPSS P = 0.042). Involvement of lipid-related genes to steatosis in portosystemic shunting was corroborated using gene-expression profiling. Lipid analysis demonstrated different triglyceride composition and a shift towards short chain and omega-3 fatty acids in shunt versus healthy dogs, with no difference in lipid species composition between shunt types. All organoids showed a similar increase in triacylglycerols after free fatty acids enrichment. This study demonstrates that steatosis is probably secondary to canine portosystemic shunts. Unravelling the pathogenesis of this hepatic steatosis might contribute to a better understanding of steatosis in NAFLD.

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Section: Discussionmentioning

confidence: 99%

Aberrant hepatic lipid storage and metabolism in canine portosystemic shunts

et al. 2017

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“…The relationship between hepatic encephalopathy and inflammation is inherent, since the cerebral edema observed in most patients with this condition is due mainly to an increase in TNF-a and IL-1b, cytokines which are responsible for an increase in vascular permeability and, consequently, for exsudate formation within the intersticial space [4,9]. Since the elucidation of this mechanism as being the most likely culprit for the morbimortality associated with hepatic encephalopathy, researchers have focused on measures which may interrupt disease progression and/or revert this state completely [36][37][38][39]. Accordingly, the first goal of this work was to evaluate brain tissue levels of pro-and anti-inflammatory cytokines in rats submitted to an experimental model of hepatic encephalopathy and to verify whether this model was adequate for evaluating inflammatory modulation in the brain of rats.…”

Section: Discussionmentioning

confidence: 99%

Carbon Tetrachloride Increases the Pro-inflammatory Cytokines Levels in Different Brain Areas of Wistar Rats: The Protective Effect of Acai Frozen Pulp

et al. 2015

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Acai offers health benefits associated with its high antioxidante capacity, phytochemical composition, nutritional and sensory value. Therefore, the objective of this study was to evaluate the protective effect of acai frozen pulp on carbon tetrachloride (CCl4)-induced damage via modulation of anti- and pro-inflammatory cytokines in rat brain tissue. The rats were treated via oral (gavage) daily with water or acai frozen pulp for 14 days at a dose of 7 μL/g. On the 15th day, the animals in each group received a single intraperitoneal injection of CCl4 in a dose of 3.0 mL/kg or the same volume of mineral oil. After 4 h, the animals were euthanized by decapitation and the cerebral cortex, hippocampus and cerebellum were dissected and homogenated to evaluate the levels of tumor necrosis factor α (TNF-α), interleukin 1β (IL-1β), interleukin 18 (IL-18), interleukin 6 (IL-6) and interleukin 10 (IL-10). Data were statistically analyzed by analysis of variance followed by the Tukey post hoc test. It was observed that CCl4 increased TNF-α, IL-1β and IL-18 levels in all brain tissues, and that acai frozen pulp was able to prevent this increase. IL-6 and IL-10 brain tissue levels remained unchanged during all treatments. CCl4 experimental model was suitable to investigate brain tissue anti and pro-inflammatory cytokines. Acai frozen pulp prevented an increase in IL-1β, IL-18 and TNF-α, while IL-6 and IL-10 levels remained unchanged. The precise pathway by which inflammation contribute to hepatic encephalopathy, as well as to how this pathway can be modulated, is still under investigation.

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“…Hypercholesterolemic group that fed on basal diet containing 15% fish oil in pizza as treatment showed decreasing in PRL hormone values compared to the group fed on basal diet containing 10% fish oil in pizza. In the same line Staziaki, et al (2013) who stated the positive effect of chronic fish oil (FO) supplementation (rich in omega-3 polyunsaturated fatty acids) on femininity hormones behavior as progesterone and oxidative stress of Wistar female rats Table (5 Each value represent the mean of 5 rats ± SD. Values significantly different compared to normal: P ≤ 0.05.…”

Section: -3-effect Of Pizza Supplemented With Different Levels Of Fish Oil On Kidney Functions Of Hypercholesterolemic Ratsmentioning

confidence: 92%

Biological Studies on Some Nutritional Products Supplemented With Fish Oil for Hyperlepidmic Rats

Helal¹,

Tahan²,

Gharib³

et al. 2015

المجلة العلمية لعلوم التربية النوعية

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Reducing intake of saturated fat, dietary cholesterol and avoiding excess calories, which can lead to obesity, remain the cornerstone of the dietary approach to decreasing risk of atherosclerotic vascular disease. During the past 20 years, there has been renewed interest in other dietary components that might favorably improve lipid profiles and reduce risk of coronary heart disease (CHD). Fish oil, rich sources of omega-3 fatty acids, have sparked intense interest in both epidemiological studies, which suggest a favorable effect on CHD, and metabolic ward studies, which show a striking improvement in lipid profiles in hyperlipidemic patients. This research aimed to study the biological properties of some nutritional products supplemented with fish oil on hyperlipidemic rats. Thirty female albino rats (30 female) , weighing 140 ±5g were divided into 6 groups and administered fish oil products daily for 28 days. Blood samples were taken from each rat and tested for total cholesterol, (LDL), (HDL), triglycerides, liver enzymes activities, kidney functions, progesterone and PRL hormone. Results showed that triglycerides, total cholesterol, LDL, VLDL, and liver enzymes activities (AST and ALT) were significantly increased, while HDL was significantly decreased in positive control group compared with the negative control rats. Treating hypercholesterolemic rats with 20%pizza contained 10% and 15% fish oil in hypercholesterolemic diet caused a significant improvement in these biochemical measures and the best results were achieved by using basal diet with 20% pizza contained (15%) fish oil . So, it could be concluded that fish oil is useful for hyperlipidemic patients and for the prevention of heart disease and hardening of the arteries.

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Fish Oil has Beneficial Effects on Behavior Impairment and Oxidative Stress in Rats Subjected to a Hepatic Encephalopathy Model

Cited by 11 publications

References 57 publications

Aberrant hepatic lipid storage and metabolism in canine portosystemic shunts

Aberrant hepatic lipid storage and metabolism in canine portosystemic shunts

Carbon Tetrachloride Increases the Pro-inflammatory Cytokines Levels in Different Brain Areas of Wistar Rats: The Protective Effect of Acai Frozen Pulp

Biological Studies on Some Nutritional Products Supplemented With Fish Oil for Hyperlepidmic Rats

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