A novel colorimetric potassium sensor based on the substitution of lead from G-quadruplex

Sun, Huijiao; Li, Xiaohong; Li, Yunchao; Fan, Louzhen; Kraatz, Heinz‐Bernhard

doi:10.1039/c2an36564b

Cited by 51 publications

(34 citation statements)

References 43 publications

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“…241–243 Conjugated polymers that could distinguish K + -bound and non-bound G-quadruplex DNA were also used for amplified fluorescence detection of K + in homogeneous solution. 244,245 Colorimetric 246–249 and electrochemical 250,251 methods were also demonstrated based on G-quadruplex DNA for K + detection.…”

Section: Mismatched Dna and G-quadruplex Dna For Metal Ion Bindingmentioning

confidence: 99%

DNA as Sensors and Imaging Agents for Metal Ions

Xiang

Lu²

2013

Inorg. Chem.

139

104

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Increasing interests in detecting metal ions in many chemical and biomedical fields have created demands for developing sensors and imaging agents for metal ions with high sensitivity and selectivity. This review covers recent progress in DNA-based sensors and imaging agents for metal ions. Through both combinatorial selection and rational design, a number of metal ion-dependent DNAzymes and metal ion-binding DNA structures that can selectively recognize specific metal ions have been obtained. By attaching these DNA molecules with signal reporters such as fluorophores, chromophores, electrochemical tags, and Raman tags, a number of DNA-based sensors for both diamagnetic and paramagnetic metal ions have been developed for fluorescent, colorimetric, electrochemical, and surface Raman detections. These sensors are highly sensitive (with detection limit down to 11 ppt) and selective (with selectivity up to millions-fold) toward specific metal ions. In addition, through further development to simplify the operation, such as the use of “dipstick tests”, portable fluorometers, computer-readable discs, and widely available glucose meters, these sensors have been applied for on-site and real-time environmental monitoring and point-of-care medical diagnostics. The use of these sensors for in situ cellular imaging has also been reported. The generality of the combinatorial selection to obtain DNAzymes for almost any metal ion in any oxidation state, and the ease of modification of the DNA with different signal reporters make DNA an emerging and promising class of molecules for metal ion sensing and imaging in many fields of applications.

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Section: Mismatched Dna and G-quadruplex Dna For Metal Ion Bindingmentioning

confidence: 99%

DNA as Sensors and Imaging Agents for Metal Ions

Xiang

Lu²

2013

Inorg. Chem.

139

104

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“…[26][27][28]33 The sensor platform presented here can also be readily applied to other targets by simply employing different aptamer sequences. Electrical measurement of potassium ions is of biological interest due to the physiological function of potassium ions including regulation of blood pressure, controlling muscular functions and maintaining osmotic pressure or enzyme activations.…”

Section: Introductionmentioning

confidence: 99%

Electrostatic gating in carbon nanotube aptasensors

et al. 2016

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Synthetic DNA aptamer receptors could boost the prospects of carbon nanotube (CNT)-based electronic biosensors if signal transduction can be understood and engineered. Here, we report CNT aptasensors for potassium ions that clearly demonstrate aptamer-induced electrostatic gating of electronic conduction. The CNT network devices were fabricated on flexible substrates via a facile solution processing route and non-covalently functionalised with potassium binding aptamers. Monotonic increases in CNT conduction were observed in response to increasing potassium ion concentration, with a level of detection as low as 10 picomolar. The signal was shown to arise from a specific aptamer-target interaction that stabilises a G-quadruplex structure, bringing high negative charge density near the CNT channel. Electrostatic gating is established via the specificity and the sign of the current response, and by observing its suppression when higher ionic strength decreases the Debye length at the CNT-water interface. Sensitivity towards potassium and selectivity against other ions is demonstrated in both resistive mode and real time transistor mode measurements. The effective device architecture presented, along with the identification of clear response signatures, should inform the development of new electronic biosensors using the growing library of aptamer receptors.

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“…All experiments were performed at room temperature. indicative of parallel K + -stabilized G-quadruplex [32,33]. The results demonstrated that the presence K + could induce the hairpin Ap-DNA structural change, forming K + -stabilized G-quadruplex.…”

Section: Electrochemical Measurementsmentioning

confidence: 80%