The effect of Phloretin on human γδ T cells killing colon cancer SW-1116 cells

Zhu, Sheng-Ping; Liu, Gang; Wu, Xiaoting; Chen, Fuxing; Liu, Junquan; Zhou, Zhonghai; Zhang, Jian‐Fu; Fei, Sujuan

doi:10.1016/j.intimp.2012.11.001

Cited by 24 publications

(11 citation statements)

References 17 publications

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“…MTT results showed that phloretin inhibited proliferation of A549, Calu-1, H838 and H520 cells in a dose-dependent manner while flow cytometry also indicated its apoptosis-induction ability in H838 and H520 cells, these results were consistent with previous research reporting the anticancer effects of phloretin on colon cancer cells (18). As we know, the imbalance between proliferation and apoptosis leads to limitless cell proliferation which is the hallmark of cancers.…”

Section: Discussionsupporting

confidence: 91%

Phloretin exhibits an anticancer effect and enhances the anticancer ability of cisplatin on non-small cell lung cancer cell lines by regulating expression of apoptotic pathways and matrix metalloproteinases

Wang

Nan

et al. 2015

International Journal of Oncology

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Abstract. Non-small cell lung cancer (NSCLC) accounts for 80-85% of all lung cancer cases and the prognosis of NSCLC patients is unsatisfactory since 5-year survival rate of NSCLC is still as low as 11%. Natural compounds derived from plants with few or no side effects have been recognized as alternative or auxiliary cure for cancer patients. Phloretin is such an agent possessing various pharmacological activities; however, there is scarce information on its anticancer effects on NSCLC. It was evaluated and confirmed, in the present study, that phloretin inhibited proliferation and induced apoptosis in A549, Calu-1, H838 and H520 cells in a dosedependent manner, phloretin also suppressed the invasion and migration of NSCLC cells. We further confirmed that phloretin dose-dependently suppressed the expression of Bcl-2, increased the protein expression of cleaved-caspase-3 and -9, and deregulated the expression of matrix metalloproteinases (MMP)-2 and -9 on gene and protein levels. Besides, evaluations revealed that phloretin enhanced the anticancer effects of cisplatin on inhibition of proliferation and induction of apoptosis in NSCLC cells. Moreover, phloretin facilitated the effects of cisplatin on deregulation of Bcl-2, MMP-2 and -9, and upregulation of cleaved-caspase-3 and -9. In conclusion, the present study demonstrated that phloretin possessed anticancer effects and enhanced the anticancer effects of cisplatin on NSCLC cell lines by suppressing proliferation, inducing apoptosis and inhibiting invasion and migration of the cells through regulating apoptotic pathways and MMPs. IntroductionLung cancer is the most frequent reason for cancer related death and non-small cell lung cancer (NSCLC) accounts for ~80-85% of all lung cancer cases (1). Although there has been notable progression in combination chemotherapy and surgical techniques, the prognosis of NSCLC patients is still unsatisfactory since 5-year survival rate of NSCLC with all stages and histological types is as low as 11% (2). Besides the prolonged life time, the survival quality of NSCLC patients should also be taken care of, while the high toxicity of anticancer drugs adopted in clinical first line to normal tissues and cells is an impassable barrier for cancer therapy and a heavy burden for patients to bear. It would be better if there were some methods that could improve the clinical therapy effects and relieve the pain of the patients.Agents derived from various plants with few or no side effects have been recognized as potential alternative or auxiliary cure for cancer patients. Flavonoids, one major class of polyphenols, are well known for their antioxidant activity by eliminating reactive oxygen species (3) and chelating metal atoms (4,5). There is also substantial research suggesting that flavonoids have anticancer effects (6). Phloretin [3-(4-hydroxyphenyl)-1-(2,4,5-trihydroxyphenyl)] is one of the major phenolic flavonoid glucosides (structure showed in Fig. 1) found in apples and other plants, such as Pieris japonica, Hoveniae Lignum and...

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Section: Discussionsupporting

confidence: 91%

Phloretin exhibits an anticancer effect and enhances the anticancer ability of cisplatin on non-small cell lung cancer cell lines by regulating expression of apoptotic pathways and matrix metalloproteinases

Wang

Nan

et al. 2015

International Journal of Oncology

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“…Different authors have investigated the capacity of phloretin to potentiate the activity of immune cells against tumor cells favoring cellular proliferation and differentiation and enhancing the cytotoxic activity up-regulating proteins such as perforin or granzyme B (Nusse and Varmus 1992;Zhu et al 2013). In other studies the treatment of cancer cells with phloretin significantly has suppressed the growth of these cancer cells.…”

Section: Aspalathus Linearis Fabaceaementioning

confidence: 99%

Health effects of phloretin: from chemistry to medicine

et al. 2017

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Dihydrochalcones are a class of secondary metabolites, whose demand in biological and pharmacological applications is rapidly growing. Phloretin is one of the best known and abundant dihydrochalcone characterized by the presence of 2,6-dihydroxyacetophenone pharmacophore. It is a versatile molecule with anticancer, antiosteoclastogenic, antifungal, antiviral, anti-inflammatory, antibacterial and estrogenic activities and able to increase the fluidity of biological membranes and penetration of administered drugs. In this review we have performed a critical evaluation of available literature as far as phloretin beneficial effects and activation/block of intracellular signal cascade are of concern. In addition, we supply useful information on its chemical properties, sources and bioavailability.

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“…Phl reduces renal glucose transport, lowering the amount of glucose in the blood. Phl and its derivatives have found wide usage in food and beverage industry as well as additive in pharmaceuticals and cosmetics (Zhu et al 2013;Kim et al 2014). There are several research articles published related to phlorizin and its derivatives' pharmacological effect (Ostroumova et al 2013;Shin et al 2011;Lee et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Highly Selective Electrochemical Determination of Phlorizin Using Square Wave Voltammetry at a Boron-Doped Diamond Electrode

et al. 2017

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A boron-doped diamond electrode was used as an electrochemical sensor for the determination of phlorizin (aka phloridzin, phlorrhizin) using square wave voltammetry (SWV). Phlorizin (Phl) exhibited a well-defined oxidation peak at +0.9 V (versus Ag/AgCl electrode 3 M KCl) in solutions with a pH value of 6.0. Parameters such as pH value and scan rate were optimized for cyclic voltammetry as well as amplitude and frequency for SWV. The sensor gave excellent response with a wide linear dynamic range for concentrations of phlorizin from 3 to 100 μM with a detection limit of 0.23 μM and a good repeatability (± 0.9%, n = 7 measurements, c = 10 μM). The effect of interferences by most common compounds was tested, and the method was successfully applied to the determination of the title compound in apple root extracts and urine samples with satisfactory recovery.

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The effect of Phloretin on human γδ T cells killing colon cancer SW-1116 cells

Cited by 24 publications

References 17 publications

Phloretin exhibits an anticancer effect and enhances the anticancer ability of cisplatin on non-small cell lung cancer cell lines by regulating expression of apoptotic pathways and matrix metalloproteinases

Phloretin exhibits an anticancer effect and enhances the anticancer ability of cisplatin on non-small cell lung cancer cell lines by regulating expression of apoptotic pathways and matrix metalloproteinases

Health effects of phloretin: from chemistry to medicine

Highly Selective Electrochemical Determination of Phlorizin Using Square Wave Voltammetry at a Boron-Doped Diamond Electrode

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