Reference interval of pregnancy-associated plasma protein-A in healthy men and non-pregnant women

Coşkun, Abdurrahman; Serteser, Mustafa; Duran, Sadik; İnal, Tamer; Erdogan, Bi̇rsen Eygi; Özpınar, Aysel; Can, Özge; Ünsal, İbrahim

doi:10.1016/j.jjcc.2012.09.007

Cited by 8 publications

(12 citation statements)

References 23 publications

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“…PAPPA is a metalloprotease belonging to the metzincin superfamily of zinc peptidase (Leguy et al 2014 ). It is an active homodimer (dPAPPA), circulating at very low levels in non-pregnant women and men, and regulates cell differentiation and proliferation by cleaving insulin-like growth factor binding proteins 4 and 5 (D’Elia et al 2012 ; Coskun et al 2013 ). During pregnancy, PAPPA is produced at high levels by the placenta and circulates as a heterotetrameric complex (htPAPPA).…”

Section: Discussionmentioning

confidence: 99%

Transcriptome profile of the human placenta

Majewska

Lipka

Paukszto

et al. 2017

Funct Integr Genomics

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The human placenta is a particular organ that inseparably binds the mother and the fetus. The proper development and survival of the conceptus relies on the essential interplay between maternal and fetal factors involved in cooperation within the placenta. In our study, high-throughput sequencing (RNA-seq) was applied to analyze the global transcriptome of the human placenta during uncomplicated pregnancies. The RNA-seq was utilized to identify the global pattern of the gene expression in placentas (N = 4) from women in single and twin pregnancies. During analyses, we obtained 228,044 transcripts. More than 91% of them were multi-exon, and among them 134 were potentially unknown protein coding genes. Expression levels (FPKM) were estimated for 38,948 transcriptional active regions, and more than 3000 of genes were expressed with FPKM >20 in each sample. Additionally, all unannotated transcripts with estimated FPKM values were localized on the human genome. Highly covered splice junctions unannotated in the human genome (6497) were identified, and among them 30 were novel. To gain a better understanding of the biological implications, the assembled transcripts were annotated with gene ontology (GO) terms. Single nucleotide variants were predicted for the transcripts assigned to each analyzed GO category. Our results may be useful for establishing a general pattern of the gene expression in the human placenta. Characterizing placental transcriptome, which is crucial for a pregnancy’s outcome, can serve as a basis for identifying the mechanisms underlying physiological pregnancy, as well as may be useful for an early detection of the genomic defects.Electronic supplementary materialThe online version of this article (doi:10.1007/s10142-017-0555-y) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Transcriptome profile of the human placenta

Majewska

Lipka

Paukszto

et al. 2017

Funct Integr Genomics

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“…These results were however not significant. Coskun et al [15] found median PAPP-A concentration in women (3.40 ng/mL) significantly lower than in men (6.85 ng/mL). When dividing the group of women into pre-and postmenopausal, the median PAPP-A concentrations were 2.60 ng/mL and 8.50 ng/mL respectively -a difference that was not significant.…”

Section: Discussionmentioning

confidence: 96%

“…Samples for measurement of PAPP-A were drawn before the first dose of heparin was given, every 15 min for the first 4½ h, and every 30 min for the following 4 h (i.e. after 15,30,45,60,75,90,105,120,135,150,165,180,195,210,225,240,255,270, 300, 330, 360, 390, 420, and 450 min), giving a total of 25 samples. In addition to serum samples for PAPP-A measurement, we also drew blood for monitoring activated partial thromboplastin time (APTT) at the same time points.…”

Section: Serum Samplesmentioning

confidence: 99%

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The effect of heparin on pregnancy associated plasma protein-A concentration in healthy, non-pregnant individuals

Jespersen

Vestergaard

Schou

et al. 2015

Clinical Biochemistry

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“…It is active as a homodimer (dPAPP-A) which cleaves insulin-like growth factor binding proteins 4 and 5, thus regulating local IGF bioavailability and hence cell differentiation and proliferation [ 3 , 4 ]. dPAPP-A circulates at very low levels in non-pregnant women and men [ 5 ]. This dPAPP-A is abundantly expressed in unstable coronary atherosclerotic plaques and can be released in the event of rupture, thus rendering dPAPP-A a biomarker for coronary syndrome and unstable angina [ 6 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

Pattern of secretion of pregnancy-associated plasma protein-A (PAPP-A) during pregnancies complicated by fetal aneuploidy, in vivo and in vitro

Leguy

Brun²,

Pidoux

et al. 2014

Reprod Biol Endocrinol

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BackgroundPregnancy-associated placental protein-A (PAPP-A) is a metalloprotease which circulates as an hetero-tetramer in maternal blood. Its maternal serum concentration in fetal trisomy 21 is decreased during the first trimester, so that PAPP-A is a useful screening biomarker. However, the regulation of PAPP-A placental secretion is unclear. We therefore investigated the secretion of PAPP-A in pregnancies complicated by fetal aneuploidies, both in vivo and in vitro.MethodsMaternal serum collected between 10 WG and 33 WG during 7014 normal pregnancies and 96 pregnancies complicated by fetal trisomy 21, 18, and 13 were assayed for PAPP-A using the Immulite 2000xpi system®. The pregnancies were monitored using ultrasound scanning, fetal karyotyping and placental analysis. Villous cytotrophoblasts were isolated from normal and trisomic placenta and cultured to investigate PAPP-A secretion in vitro (n = 6).ResultsAn increased nuchal translucency during the first trimester is a common feature of many chromosomal defect but each aneuploidy has its own syndromic pattern of abnormalities detectable at the prenatal ultrasound scanning and confirmed at the fetal examination thereafter. PAPP-A levels rise throughout normal pregnancy whereas in trisomy 21, PAPP-A levels were significantly decreased, but only during the first trimester. PAPP-A levels were decreased in trisomy 13 and sharply in trisomy 18, whatever the gestational age. In vitro, PAPP-A secretion was decreased in aneuploidy, and associated with decreased hCG secretion in Trisomy 21 and 18. These biochemical profiles did not appear to be linked to any specific histological lesions affecting the placenta.ConclusionsThese profiles may reflect different quantitative and qualitative placental dysfunctions in the context of these aneuploidies.

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Reference interval of pregnancy-associated plasma protein-A in healthy men and non-pregnant women

Abstract: The reference interval of PAPP-A differed for men and non-pregnant women. In clinical practice, <22.9 ng/mL for men and <33.6 ng/mL for non-pregnant women may be used as reference intervals for PAPP-A.

Cited by 8 publications

References 23 publications

Transcriptome profile of the human placenta

Transcriptome profile of the human placenta

The effect of heparin on pregnancy associated plasma protein-A concentration in healthy, non-pregnant individuals

Pattern of secretion of pregnancy-associated plasma protein-A (PAPP-A) during pregnancies complicated by fetal aneuploidy, in vivo and in vitro

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