Effects of various cryoprotectants on bull sperm quality, DNA integrity and oxidative stress parameters

Taşdemir, Umut; Büyükleblebici, Serhat; Tuncer, Pürhan Barbaros; Coşkun, Erdem; Özgürtaş, Taner; Aydın, Fevzi Nuri; Büyükleblebici, Olga; Gürcan, İsmayil Safa

doi:10.1016/j.cryobiol.2012.10.006

Cited by 67 publications

(38 citation statements)

References 44 publications

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“…Whether the cryoprotectants are able to interfere in this mechanism remains to be fully elucidated. Thus, various extenders and cryoprotective agents have been investigated for the cryopreservation of ram semen (Nur et al, 2010;Najafi et al, 2013;Taşdemir et al, 2013). Previous studies have demonstrated the cryoprotectant properties of soybean-derived lecithin for protection of ram semen during freeze-thawing processes (MataCampuzano et al, 2015;Mehdipour et al, 2016;Najafi et al, 2014b;Toker et al, 2016).…”

Section: Discussionmentioning

confidence: 98%

Ethylene glycol, but not DMSO, could replace glycerol inclusion in soybean lecithin-based extenders in ram sperm cryopreservation

Najafi

Kia

Dodaran

et al. 2017

Animal Reproduction Science

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Section: Discussionmentioning

confidence: 98%

Ethylene glycol, but not DMSO, could replace glycerol inclusion in soybean lecithin-based extenders in ram sperm cryopreservation

Najafi

Kia

Dodaran

et al. 2017

Animal Reproduction Science

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“…DMSO could conceivably cause DNA damage, as demonstrated by DNA repair mutant sensitivity (Figure 4A). DMSO damaged DNA in bull sperm (Taşdemir et al, 2013) and erythroleukemic cells (Scher and Friend, 1978), and additionally altered expression of DNA repair genes in human and rat hepatocytes (Sumida et al, 2011). …”

Section: Discussionmentioning

confidence: 99%

Functional genomics indicates yeast requires Golgi/ER transport, chromatin remodeling, and DNA repair for low dose DMSO tolerance

Gaytán¹,

Loguinov²,

Rosa³

et al. 2013

Front. Genet.

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Dimethyl sulfoxide (DMSO) is frequently utilized as a solvent in toxicological and pharmaceutical investigations. It is therefore important to establish the cellular and molecular targets of DMSO in order to differentiate its intrinsic effects from those elicited by a compound of interest. We performed a genome-wide functional screen in Saccharomyces cerevisiae to identify deletion mutants exhibiting sensitivity to 1% DMSO, a concentration standard to yeast chemical profiling studies. We report that mutants defective in Golgi/ER transport are sensitive to DMSO, including those lacking components of the conserved oligomeric Golgi (COG) complex. Moreover, strains deleted for members of the SWR1 histone exchange complex are hypersensitive to DMSO, with additional chromatin remodeling mutants displaying a range of growth defects. We also identify DNA repair genes important for DMSO tolerance. Finally, we demonstrate that overexpression of histone H2A.Z, which replaces chromatin-associated histone H2A in a SWR1-catalyzed reaction, confers resistance to DMSO. Many yeast genes described in this study have homologs in more complex organisms, and the data provided is applicable to future investigations into the cellular and molecular mechanisms of DMSO toxicity.

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“…The lowest VCL values were detected in CLC and 7-DHCLC with 3% G. In bulls, the importance of VCL in fertilization capacity of spermatozoa has been well documented [31] . Therefore, these values indicate that sperm classified as highly mobile swims faster than those classified to have lower mobility [38] . Thus, the role of VCL in sperm transportation may be during the passage through the female reproductive tract and penetration to the oocyte vestments.…”

Section: Discussionmentioning

confidence: 99%

Kolestrol ve 7-Dehidrokolestrolün Farkli Gliserol Oranlari İle Boğa Spermasi Dondurulmasina Etkisi

̇İnanç¹,

Olğaç²,

Tekin³

et al. 2018

Kafkas Univ Vet Fak Derg

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How to Cite This Articleİnanç ME, Olgac KT, Tekin K, Çil B, Alemdar H, Özen D, Uysal O: Effect of cholesterol and 7-dehydrocholesterol on bull semen freezing with different rates of glycerol. Kafkas Univ Vet Fak Derg, 24 (6): 815-820, 2018. DOI: 10.9775/kvfd.2018 AbstractThe aim of this study was to evaluate the effect of 7-dehydrocholesterol loaded cyclodextrin (7-DHCLC) and cholesterol loaded cyclodextrin (CLC) with the addition of different glycerol (G) rates on bull semen cryopreservation. Semen samples of three bulls were pooled and divided into seven groups; control group (C) and the other six were extended with standard Tris extender including different G rates (3, 6 and 9%) with CLC or 7-DHCLC to reach 1.5 mg/120x10 6 . Extended samples were frozen in 0.25 mL straws. After thawing, motion characteristics and motility, viability, acrosome integrity, mitochondrial activation, functional membrane integrity and abnormal spermatozoa rates were assessed. The highest membrane integrity, viability and total motility were detected in 7-DHCLC with 9% of G group (P<0.001). The lowest progressive motility was detected in 3% G groups (P<0.001). 7-DHCLC with 9% G had higher mitochondrial activity compared with 3% G groups. In addition, no statistical difference was observed between groups in terms of acrosome integrity and abnormal spermatozoa rates (P>0.05). According to these results, 7-DHCLC with 9% G addition maintained some spermatological parameters better than other groups after thawing in bull semen thus, it was concluded that 7-DHCLC could be used instead of CLC in bull semen cryopreservation. Keywords: Cholesterol 7-dehydrocholesterol, Cyclodextrin, Glycerol, Bull semen, Freeze-thawed Kolestrol ve 7-Dehidrokolestrolün Farklı Gliserol Oranları İle Boğa Sperması Dondurulmasına Etkisi ÖzBu çalışmanın amacı, 7-dehidrokolesterol yüklü siklodekstrin (7-DHCLC) ve kolesterol yüklü siklodekstrin (CLC) 'in farklı gliserol (G) oranları ilavesi ile boğa sperması dondurulması üzerindeki etkilerini değerlendirmektir. Üç adet boğaya ait sperma örnekleri toplandı, birleştirildi ve yedi gruba ayrıldı; biri kontrol (C) ile, diğer altı grup ise CLC veya 7-DHCLC (1.5 mg/120x10 6 ) ve farklı G oranlarını (%3, 6 ve 9) içeren standart Tris sulandırıcısı ile sulandırıldı. Sulandırılan spermalar 0.25 mL'lik payetlerde donduruldu. Çözdürme sonrası gruplar hareket karakteristikleri ve motilite, viabilite, akrozom bütünlüğü, mitokondriyal aktivasyon, fonksiyonel membran bütünlüğü ve anormal spermatozoa oranları yönünden değerlendirildi. En yüksek membran bütünlüğü, viabilite ve motilite %9 G ilavesi ile 7-DHCLC grubunda tespit edildi (P<0.001). En düşük progresif motilite %3 G gruplarında saptandı (P<0.001). %9 G içeren 7-DHCLC grubu, %3 G gruplarına kıyasla daha yüksek mitokondriyal aktivite belirlendi. Ayrıca, gruplar arasında akrozom bütünlüğü ve anormal spermatozoa oranları açısından istatistiksel bir fark gözlenmedi (P>0.05). Bu sonuçlara göre, %9 G ilave edilen 7-DHCLC grubunun, diğer gruplara kıyasla eritme sonrası bazı spermat...

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Effects of various cryoprotectants on bull sperm quality, DNA integrity and oxidative stress parameters

Cited by 67 publications

References 44 publications

Ethylene glycol, but not DMSO, could replace glycerol inclusion in soybean lecithin-based extenders in ram sperm cryopreservation

Ethylene glycol, but not DMSO, could replace glycerol inclusion in soybean lecithin-based extenders in ram sperm cryopreservation

Functional genomics indicates yeast requires Golgi/ER transport, chromatin remodeling, and DNA repair for low dose DMSO tolerance

Kolestrol ve 7-Dehidrokolestrolün Farkli Gliserol Oranlari İle Boğa Spermasi Dondurulmasina Etkisi

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