Antibody response to Mycobacterium tuberculosis p27-PPE36 antigen in sera of pulmonary tuberculosis patients

Moigne, Vincent Le; Moigne, Delphine Le; Mahana, Wahib

doi:10.1016/j.tube.2012.10.006

Cited by 7 publications

(5 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…5d, panels L and R). Elevated specific IgA in the absence of specific IgG was also observed in a study on Rv2018 [37]. Antigens of Plasmodium falciparum were reported to induce the production of a specific IgG subclass in humans [38].…”

Section: Discussionmentioning

confidence: 81%

Comparison of antibody responses against Mycobacterium tuberculosis antigen Rv0679c in tuberculosis patients from the endemic and non-endemic regions of the Beijing genotype: a case control study

et al. 2017

View full text Add to dashboard Cite

BackgroundStrains of the Beijing genotype of Mycobacterium tuberculosis (MTB) are reportedly associated with the virulence of tuberculosis (TB) infection, unfavorable outcomes of anti-TB treatment, and the global TB pandemic. Rv0679c, a hypothetical membrane protein related to host cell invasion, has a Beijing genotype-specific mutation at residue 142 (Asn142Lys). Antigenicity differences between Rv0679c-Asn142 (N-type) and Rv0679c-Lys142 (K-type) have been previously observed in mice antigen-antibody responses. However, the immune response to Rv0679c in humans remains unknown. Therefore, we aimed to investigate the anti-Rv0679c immune response in TB patients from the endemic and non-endemic regions of the Beijing MTB genotype.MethodsWe analyzed the Rv0679c-specific antibody responses in 84 subjects from the endemic region of the Beijing genotype MTB in China, including 45 pulmonary TB patients (C-PTB) and 39 healthy controls (C-HC), and 81 subjects from the Philippines (the endemic region of the non-Beijing genotype), including 51 pulmonary TB patients (P-PTB) and 30 healthy controls (P-HC). Anti-tuberculous-glycolipid (TBGL) antigen was used as the control antibody.ResultsTBGL IgG titers were higher in both C-PTB and P-PTB than those in their corresponding HC (C-PTB median 4.2, P-PTB median 11.2; C-PTB vs. P-PTB, p > 0.05), suggesting immune response comparability in PTB from two different countries. C-PTB showed a higher response compared to C-HC for anti-K-type IgG (53.3%) than anti-N-type IgG (6.67%); this response was not observed in P-PTB (both N-type and K-type 9.80%).ConclusionDimorphic antigen Rv0679c was found to be associated with distinct immune response patterns, indicating the role of Beijing/non-Beijing genotype of MTB in stimulating specific responses in TB patients from the endemic region of Beijing MTB. Meanwhile, reactions to Rv0679c in patients and HC from non-endemic regions of the Beijing MTB may be caused by the response to the common epitope of Rv0679c N/K-type.

show abstract

Section: Discussionmentioning

confidence: 81%

Comparison of antibody responses against Mycobacterium tuberculosis antigen Rv0679c in tuberculosis patients from the endemic and non-endemic regions of the Beijing genotype: a case control study

et al. 2017

View full text Add to dashboard Cite

show abstract

“…In a previous study, the recombinant p27-PPE36 (Rv2108) protein is immunologically active and reacts with antibodies in the sera of TB patients, as detected by ELISA assay [97]. It is worthwhile to note that the presence of IgA and absence of IgG antibody responses can be used as the specific diagnosis markers for Mtb [98]. The current transcriptomic data indicated that the expression levels of PPE36 were upregulated more than 3-fold in V B1 -treated cells (Additional file 2: Table S2).…”

Section: Resultsmentioning

confidence: 99%

The prominent alteration in transcriptome and metabolome of Mycobacterium bovis BCG str. Tokyo 172 induced by vitamin B1

Song¹,

Li²,

Cui³

et al. 2019

BMC Microbiol

View full text Add to dashboard Cite

Background Vitamin B 1 (V B1 ) is a crucial dietary nutrient and essential cofactor for several key enzymes in the regulation of cellular and metabolic processes, and more importantly in the activation of immune system. To date, the precise role of V B1 in Mycobacterium tuberculosis remains to be fully understood. Results In this study, the transcriptional and metabolic profiles of V B1 -treated Mycobacterium. bovis BCG were analyzed by RNA-sequencing and LC-MS (Liquid chromatography coupled to mass spectrometry). The selection of BCG strain was based on its common physiological features shared with M. tuberculosis . The results of cell growth assays demonstrated that V B1 inhibited the BCG growth rate in vitro. Transcriptomic analysis revealed that the expression levels of genes related to fatty acid metabolism, cholesterol metabolism, glycolipid catabolism, DNA replication, protein translation, cell division and cell wall formation were significantly downregulated in M. bovis BCG treated with V B1. In addition, the metabolomics LC-MS data indicated that most of the amino acids and adenosine diphosphate (ADP) were decreased in M. bovis BCG strain after V B1 treatment. Conclusions This study provides the molecular and metabolic bases to understand the impacts of V B1 on M.bovis BCG . Electronic supplementary material The online version of this article (10.1186/s12866-019-1492-9) contains supplementary material, which is available to authorized users.

show abstract

“…The importance of the IgA response against mycobacterial pathogens has been demonstrated in several studies ( 6 – 10 , 19 ). Both mucosal and systemic IgA have protective effects and can trigger pro-inflammatory response ( 20 , 21 ).…”

Section: Discussionmentioning

confidence: 96%

IgA Serological Response for the Diagnosis of Mycobacterium abscessus Infections in Patients with Cystic Fibrosis

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

Antibody response to Mycobacterium tuberculosis p27-PPE36 antigen in sera of pulmonary tuberculosis patients

Cited by 7 publications

References 15 publications

Comparison of antibody responses against Mycobacterium tuberculosis antigen Rv0679c in tuberculosis patients from the endemic and non-endemic regions of the Beijing genotype: a case control study

Comparison of antibody responses against Mycobacterium tuberculosis antigen Rv0679c in tuberculosis patients from the endemic and non-endemic regions of the Beijing genotype: a case control study

The prominent alteration in transcriptome and metabolome of Mycobacterium bovis BCG str. Tokyo 172 induced by vitamin B1

IgA Serological Response for the Diagnosis of Mycobacterium abscessus Infections in Patients with Cystic Fibrosis

Contact Info

Product

Resources

About