Motor outcome and allodynia are largely unaffected by novel olfactory ensheathing cell grafts to repair low-thoracic lesion gaps in the adult rat spinal cord

Deumens, Ronald; Gorp, Sebastiaan van; Bozkurt, Ahmet; Beckmann, Christina; Führmann, Tobias; Montzka, Katrin; Tolba, René; Kobayashi, Eiji; Heschel, I.; Weis, Joachim; Brook, Gary

doi:10.1016/j.bbr.2012.09.036

Cited by 21 publications

(19 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previously, it was reported that thoracic spinal cord injury does not affect the BOS of the front paws but leads to an increase in the BOS of the hind paws (Deumens et al, 2013;Hamers et al, 2001). In our study, we observed the opposite: lower BOS values and higher paw angles reflected the position of the hind paws of injured rats with knees close to each other and paws facing outwards.…”

Section: Influence Of Gdnf Delivery On the Gait Of Spinal Cord Injurecontrasting

confidence: 51%

Injectable alginate hydrogel loaded with GDNF promotes functional recovery in a hemisection model of spinal cord injury

Ansorena

Berdt

Ucakar

et al. 2013

International Journal of Pharmaceutics

View full text Add to dashboard Cite

We hypothesized that local delivery of GDNF in spinal cord lesion via an injectable alginate hydrogel gelifying in situ would support spinal cord plasticity and functional recovery. The GDNF release from the hydrogel was slowed by GDNF encapsulation in microspheres compared to non-formulated GDNF (free GDNF). When injected in a rat spinal cord hemisection model, more neurofilaments were observed in the lesion when the rats were treated with free GDNF-loaded hydrogels. More growing neurites were detected in the tissues surrounding the lesion when the animals were treated with GDNF microsphere-loaded hydrogels. Intense GFAP (astrocytes), low βIII tubulin (neural cells) and RECA-1 (endothelial cells) stainings were observed for non-treated lesions while GDNF-treated spinal cords presented less GFAP staining and more endothelial and nerve fiber infiltration in the lesion site. The animals treated with free GDNF-loaded hydrogel presented superior functional recovery compared with the animals treated with the GDNF microsphere-loaded hydrogels and non-treated animals.

show abstract

Section: Influence Of Gdnf Delivery On the Gait Of Spinal Cord Injurecontrasting

confidence: 51%

Injectable alginate hydrogel loaded with GDNF promotes functional recovery in a hemisection model of spinal cord injury

Ansorena

Berdt

Ucakar

et al. 2013

International Journal of Pharmaceutics

View full text Add to dashboard Cite

show abstract

“…All reviewed parameters are listed in Table . Table contains all publications included in the parameter analysis of this review and further indicates which parameters were analyzed in each publication (Abada, Schreiber, & Ellenbroek, ; Baiguera et al., ; Balkaya, Kröber, Gertz, Peruzzaro, & Endres, ; Cao et al., ; Cendelín, Voller, & Vozeh, ; Chen, Tsai, Lin, et al., ; Cheng et al., ; Cho, Nguyen, Satkunendrarajah, Branch, & Fehlings, ; Chuang et al., ; Côté, Detloff, Wade, Lemay, & Houlé, ; Deumens, Jaken, Marcus, & Joosten, ; Deumens et al., , ; Encarnacion et al., ; Ferdinandusse et al., ; Gabriel, Marcus, Walenkamp, & Joosten, ; Gensel et al., ; Hausner et al., ; Hetze, Römer, Teufelhart, Meisel, & Engel, ; Hill, Brodak, & Bartlett Bunge, ; Hilton et al., ; Hoffmann et al., ; Jeong et al., ; Lee, McKeon, & Bellamkonda, ; Lin et al., ; Matsuura et al., ; Mountney, Leung, Pedersen, Shear, & Tortella, ; Neumann et al., ; Petrosyan et al., ; Schira et al., ; Sheu et al., ; Singh, Murray, & Houle, ; Sucher et al., ; Truin et al., ; Vandeputte et al., ; Vlamings et al., ; Wang et al., , ; Westin, Janssen, Sager, & Temel, ; Yamamoto, Okui, Tatebe, Shinohara, & Hirata, ). Here 22 out of 42 analyzed papers showed high reliability and validity of at least one parameter measured by the catwalk.…”

Section: Resultsmentioning

confidence: 99%

“…All reviewed parameters are listed in Table 1. Table 2 contains all publications included in the parameter analysis of this review and further indicates which parameters were analyzed in each publication (Abada, Schreiber, & Ellenbroek, 2013;Baiguera et al, 2012;Balkaya, Kröber, Gertz, Peruzzaro, & Endres, 2013;Cao et al, 2008;Cendelín, Voller, & Vozeh, 2010;Chen, Tsai, Lin, et al, 2012b;Cheng et al, 1997;Cho, Nguyen, Satkunendrarajah, Branch, & Fehlings, 2012;Chuang et al, 2010;Côté, Detloff, Wade, Lemay, & Houlé, 2012;Deumens, Jaken, Marcus, & Joosten, 2007;Deumens et al, 2006Deumens et al, , 2013Encarnacion et al, 2011;Ferdinandusse et al, 2008;Gabriel, Marcus, Walenkamp, & Joosten, 2009;Gensel et al, 2006;Hausner et al, 2012;Hetze, Römer, Teufelhart, Meisel, & Engel, 2012;Hill, Brodak, & Bartlett Bunge, 2012;Hilton et al, 2013;Hoffmann et al, 2010;Jeong et al, 2011;Lee, McKeon, & Bellamkonda, 2010;Lin et al, 2010;Matsuura et al, 2013;Mountney, Leung, Pedersen, Shear, & Tortella, 2013;Neumann et al, 2009;Petrosyan et al, 2013;Schira et al, 2012;Sheu et al, 2012;Singh, Murray, & Houle, 2011;Sucher et al, 2010;…”

Section: Resultsmentioning

confidence: 99%

Validity and reliability of the CatWalk system as a static and dynamic gait analysis tool for the assessment of functional nerve recovery in small animal models

et al. 2017

View full text Add to dashboard Cite

IntroductionA range of behavioral testing paradigms have been developed for the research of central and peripheral nerve injuries with the help of small animal models. Following any nerve repair strategy, improved functional outcome may be the most important evidence of axon regeneration. A novel automated gait analysis system, the CatWalk™, can measure dynamic as well as static gait patterns of small animals. Of most interest in detecting functional recovery are in particular dynamic gait parameters, coordination measures, and the intensity of the animals paw prints. This article is designed to lead to a more efficient choice of CatWalk parameters in future studies concerning the functional evaluation of nerve regeneration and simultaneously add to better interstudy comparability.MethodsThe aims of the present paper are threefold: (1) to describe the functional method of CatWalk gait analysis, (2) to characterize different parameters acquired by CatWalk gait analysis, and to find the most frequently used parameters as well as (3) to compare their reliability and validity throughout the different studies.ResultsIn the reviewed articles, the most frequently used parameters were Swing Duration (30), Print Size (27), Stride Length (26), and Max Contact Area (24). Swing Duration was not only frequently used but was also the most reliable and valid parameter. Therefore, we hypothesize that Swing Duration constitutes an important parameter to be chosen for future studies, as it has the highest level of reliability and validity.ConclusionIn conclusion, CatWalk can be used as a complementary approach to other behavioral testing paradigms to assess clinically relevant behavioral benefits, with the main advantage that this system demonstrates both static and dynamic gait parameters at the same time. Due to limited reliability and validity of certain parameters, we recommend that only the most frequently assessed parameters should be used in the future.

show abstract

“…While migration of transplanted OECs is often limited, some studies showed that OECs have migrated much further than the immediate injury site which may be due to their attraction to reactive astrocytes (Chuah et al, ). Other studies have shown that OEC transplantation leads to no functional improvement (Centenaro et al, ; Deumens et al, ) and that migration in the injured spinal cord can be quite limited. In a canine model of spinal cord injury, OEC transplantation resulted in some improvements of fore‐hind limb coordination, but no changes in functionality of the long tracts in the spinal cord, which was attributed to limited OEC migration (Granger et al, ).…”

Section: Repairing Spinal Cord Injury By Transplanting Oecsmentioning

confidence: 96%

Crucial Roles for Olfactory Ensheathing Cells and Olfactory Mucosal Cells in the Repair of Damaged Neural Tracts

Ekberg

John

2013

The Anatomical Record

View full text Add to dashboard Cite

Olfactory ensheathing cells, the glial cells of the olfactory nervous system, exhibit unique growth-promoting and migratory properties that make them interesting candidates for cell therapies targeting neuronal injuries such as spinal cord injury. Transplantation of olfactory cells is feasible and safe in humans; however, functional outcomes are highly variable with some studies showing dramatic improvements and some no improvements at all. We propose that the reason for this is that the identity and purity of the cells is different in each individual study. We have shown that olfactory ensheathing cells are not a uniform cell population and that individual subpopulations of OECs are present in different regions of the olfactory nervous system, with strikingly different behaviors. Furthermore, the presence of fibroblasts and other cell types in the transplant can dramatically alter the behavior of the transplanted glial cells. Thus, a thorough characterization of the differences between olfactory ensheathing cell subpopulations and how the behavior of these cells is affected by the presence of other cell types is highly warranted. Anat Rec, 297:121-128, 2014. V C 2013 Wiley Periodicals, Inc.Key words: tissue engineering; stem cell; olfactory mucosal cells REPAIRING SPINAL CORD INJURY BY TRANSPLANTING OECSNeural injuries are almost always serious with lifechanging consequences. Injuries to the central nervous system (CNS) are particularly devastating as central neural tracts cannot regenerate themselves after injury. Therefore, many serious spinal cord injuries lead to permanent disability. The main problem arising after injury to the CNS is the fact that newly sprouting axons cannot find their target due to the presence of a glial scar, inflammatory mediators, inhibitory factors and reactive oxygen species (Hulsebosch, 2002;Jones et al., 2003). To date, there are no therapies that can address this problem, but one methodology that has shown encouraging results for spinal cord injury is to transplant glial cells, the supporting cells of the nervous system which are crucial for the survival of all types of neurons, and for extension of axons.Transplantation of the glial cells of the olfactory nervous system, olfactory ensheathing cells (OECs) has shown promise in both animals and humans with spinal cord injury. In humans, transplantation of OECs that

show abstract

Motor outcome and allodynia are largely unaffected by novel olfactory ensheathing cell grafts to repair low-thoracic lesion gaps in the adult rat spinal cord

Cited by 21 publications

References 24 publications

Injectable alginate hydrogel loaded with GDNF promotes functional recovery in a hemisection model of spinal cord injury

Injectable alginate hydrogel loaded with GDNF promotes functional recovery in a hemisection model of spinal cord injury

Validity and reliability of the CatWalk system as a static and dynamic gait analysis tool for the assessment of functional nerve recovery in small animal models

Crucial Roles for Olfactory Ensheathing Cells and Olfactory Mucosal Cells in the Repair of Damaged Neural Tracts

Contact Info

Product

Resources

About