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Jaworowski, Åsa; Arner, Anders

doi:10.1023/a:1005377016177

Cited by 13 publications

(2 citation statements)

References 40 publications

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“…We included a large elastic artery (aorta), muscular arteries (mesenteric artery and femoral artery), intestinal smooth muscle (longitudinal muscle of ileum), and the urinary bladder (detrusor muscle). The following values for the maximal shortening velocity ( V max , in muscle lengths per second, ML/s, at 20–22 °C) in the different tissues can be obtained from the literature: (1) aorta (0.015–0.07 ML/s in guinea pig and mouse [ 34 , 43 ], (2) muscular artery (mouse caudal artery ~ 0.08 ML/s at 37 °C [ 13 ], corresponding to about 0.02 at 22 °C using Q 10 of 2.2 from [ 24 ]), (3) intestine (mouse ileum at 37 °C 0.29 ML/s [ 44 ], corresponding to 0.09 ML/s at 22 °C; guinea pig ileum and taenia coli 0.11–0.20 ML/s [ 33 , 34 , 36 ]), and (4) urinary bladder (mouse 0.19 ML/s [ 47 ]). The examined muscles in the present study seem to be divided into slow arterial (aorta, muscular artery) and fast visceral (bladder) muscle types.…”

Section: Discussionmentioning

confidence: 99%

Signaling and metabolic properties of fast and slow smooth muscle types from mice

Boberg

Szekeres

Arner

2018

Pflugers Arch - Eur J Physiol

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This study aims to improve the classification of smooth muscle types to better understand their normal and pathological functional phenotypes. Four different smooth muscle tissues (aorta, muscular arteries, intestine, urinary bladder) with a 5-fold difference in maximal shortening velocity were obtained from mice and classified according to expression of the inserted myosin heavy chain (SMHC-B). Western blotting and quantitative PCR analyses were used to determine 15 metabolic and 8 cell signaling key components in each tissue. The slow muscle type (aorta) with a 12 times lower SMHC-B had 6-fold lower expression of the phosphatase subunit MYPT1, a 7-fold higher expression of Rhokinase 1, and a 3-fold higher expression of the PKC target CPI17, compared to the faster (urinary bladder) smooth muscle. The slow muscle had higher expression of components involved in glucose uptake and glycolysis (type 1 glucose transporter, 3 times; hexokinase, 13 times) and in gluconeogenesis (phosphoenolpyruvate carboxykinase, 43 times), but lower expression of the metabolic sensing AMP-activated kinase, alpha 2 isoform (5 times). The slow type also had higher expression of enzymes involved in lipid metabolism (hormone-sensitive lipase, 10 times; lipoprotein lipase, 13 times; fatty acid synthase, 6 times; type 2 acetyl-coenzyme A carboxylase, 8 times). We present a refined division of smooth muscle into muscle types based on the analysis of contractile, metabolic, and signaling components. Slow compared to fast smooth muscle has a lower expression of the deactivating phosphatase and upregulated Ca2+ sensitizing pathways and is more adapted for sustained glucose and lipid metabolism.

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Section: Discussionmentioning

confidence: 99%

Signaling and metabolic properties of fast and slow smooth muscle types from mice

Boberg

Szekeres

Arner

2018

Pflugers Arch - Eur J Physiol

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“…This is similar to, but a little higher than, the value of 1.7 reported in permeabilized portal vein (Mitsui et al 1994), perhaps due to differences between intact and permeabilized preparations. In intact preparations, data for force development indicates that the temperature sensitivity of force production is not limited to MLCK activity (Klemt et al 1981; Jaworowski and Arner 1998). In both ureteric preparations, however, a much more marked effect on the rate of relaxation than contraction was found; Q 10 's of 5.1 in the guinea pig and 3.7 in the rat ureter, and 3.9 was found in the portal vein (17).…”

Section: Discussionmentioning

confidence: 99%

On the Mechanisms Whereby Temperature Affects Excitation-Contraction Coupling in Smooth Muscle

Burdyga

Wray

2002

The Journal of General Physiology

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Moderate cooling of smooth muscle can modulate force production and may contribute to pathophysiological conditions, but the mechanisms underlying its effects are poorly understood. Interestingly, cooling increases force in rat ureter, but decreases it in guinea pigs. Therefore, this study used ureteric smooth muscle as a model system to elucidate the mechanisms of the effects of cooling on excitation-contraction coupling. Simultaneous recordings of force, intracellular [Ca2+], and electrical activity were made in intact ureter and ionic currents measured in isolated cells. The increase in force amplitude in rat ureter with cooling was found to be due to a significant increase in the duration of the Ca2+ transient. This in turn was due to a marked prolongation of the action potential. In guinea pigs, both these parameters were much less affected by cooling. Examination of membrane currents revealed that differences in ion channel contribution to the action potential underlie these differences. In particular, cooling potentiated Ca2+-activated Cl− currents, which are present in rat but not guinea pig ureteric smooth muscle, and prolonged the plateau of the action potential and Ca2+ entry. The force-Ca2+ relationship revealed that the increased duration of the Ca2+ transient was sufficient in the rat, but not in the guinea pig, to overcome kinetic lags produced in both species by cooling and potentiate force. Ca2+ entry and release processes were largely temperature-insensitive, but the rate of relaxation was very temperature-sensitive. Effects of cooling on myosin light chain phosphatase, confirmed in experiments using calyculin A, appear to be the predominant mechanisms affecting relaxation. Thus, smooth muscle is diverse in its response to temperature, even when experimental variables, such as the mode of stimulation, are removed. Although the biochemical and mechanical events accompanying contraction are likely to be affected in similar ways by temperature, differences in electrical events lead to subsequent differences in these processes between smooth muscles.

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Effects of temperature on vibration‐induced damage in nerves and arteries

et al. 2005

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Vasospastic episodes in hand-arm vibration syndrome are more prevalent among power-tool workers in cold climates. To test whether cold enhances vibration-induced damage in arteries and nerves, tails of Sprague-Dawley rats were vibrated at room temperature (RT) or with tail cooling (<15 degrees C). Cold vibration resulted in a colder tail than either treatment alone. Vibration at both temperatures reduced arterial lumen size. RT vibration generated more vacuoles in arteries than cold vibration. Vibration and cold induced nitration of tyrosine residues in arteries, suggesting free-radical production. Vibration and cold generated similar percentages of myelinated axons with disrupted myelin. Cold with and without vibration caused intraneural edema and dilation of arterioles and venules with blood stasis, whereas vibration alone did not. The similarities, differences, and interactive effects of cold and vibration on nerve and artery damage indicate that temperature is involved mechanistically in the pathophysiology of hand-arm vibration syndrome.

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Cited by 13 publications

References 40 publications

Signaling and metabolic properties of fast and slow smooth muscle types from mice

Signaling and metabolic properties of fast and slow smooth muscle types from mice

On the Mechanisms Whereby Temperature Affects Excitation-Contraction Coupling in Smooth Muscle

Effects of temperature on vibration‐induced damage in nerves and arteries

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