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Abstract

This study was designed to isolate new genes related to apoptosis in rat pheochromocytoma (PC12) cells treated with hydrogen peroxide (H2O2), and to characterize the roles of the genes using both in vitro and in vivo models of oxidative injury. cDNA libraries were prepared from H2O2-treated and -untreated PC12 cells, and a ribosomal protein S9 (RPS9) clone was isolated by a differential screening method. Increase of RPS9 expression in both H2O2-treated PC12 and neuroblastoma (Neuro-2A) cells was shown by Northern blot analysis. Viability of the antisense-transfected Neuro-2A (RPS9-AS) cells following H2O2 treatment was significantly reduced in a dose-dependent manner. In an in vivo model of transient forebrain ischemia, an increase in RPS9 expression was prominent by 1 day postischemia in the granule cell layer neurons of the dentate gyrus. Both activation of caspase-3 and significant recovery of viability following pretreatment with cycloheximide were shown in RPS9-AS cells treated with H2O2. These data suggest that RPS9 plays a protective role in oxidative injury of neuronal cells.

Alteration of mRNA 5-Methylcytosine Modification in Neurons After OGD/R and Potential Roles in Cell Stress Response and Apoptosis

“…Our research found that after OGD/R, the hypermethylated transcript functions were mainly enriched in pathological processes, including Huntington's disease and intrinsic apoptotic signaling pathways, whereas the hypomethylated transcript functions were mainly enriched in physiological processes. Moreover, the hub genes RPS9 and RPL8 of PPI network analysis were significantly increased in the brain tissue after cerebral ischemia and hemorrhage (Kim et al, 2003;Chen et al, 2019a). These findings indicate that m 5 C modification may also be involved in the pathological processes of cerebral injury.…”

“…Heat shock protein family A (Hsp70) member 8 (HSPA8) played an important role in the occurrence and development of neurological diseases, such as Alzheimer's disease and Parkinson's disease (Lauterbach, 2013;Silva et al, 2014). Ribosomal protein L8 (RPL8) expression levels were significantly increased in the brain tissue after cerebral hemorrhage, and Ribosomal protein S9 (RPS9)expression was increased significantly in the neurons of the dentate gyrus after acute cerebral ischemia (Kim et al, 2003;Chen et al, 2019a). By analyzing the functions of the main gene modules through MCODE, we found that the main methylation gene modules were significantly related to basic life activities of cells, including gene expression, biosynthetic processes, energy metabolism and transport (Figure 5F), and these results were similar to previous studies (Yang et al, 2019).…”

SILAC-Based Proteomic Profiling of the Human MDA-MB-231 Metastatic Breast Cancer Cell Line in Response to the Two Antitumoral Lactoferrin Isoforms: The Secreted Lactoferrin and the Intracellular Delta-Lactoferrin

“…ALDH18A1 is a protein implicated in proline and ornithine biosynthesis, and its expression is responsible for cellular ROS downregulation [61]. RPS9 is implicated in ribosomal biogenesis, has a protective role in the apoptotic process and exerts a protective mechanism against oxidative injury [62]. Interestingly, these proteins are all involved in protection against oxidative stress.…”