Human papillomavirus modifies the prognostic significance of T stage and possibly N stage in tonsillar cancer

Hong, Angela; Martin, Andrew; Armstrong, Bruce K.; Lee, Cheok Soon; Jones, D. Brian; Chatfield, Mark D.; Zhang, Mei; Harnett, Gerald B.; Clark, Jonathan R.; Elliott, Michael; Milross, Christopher G.; Smee, Robert; Corry, June; Liu, Chen; Porceddu, Sandro; Vaska, K; Veness, Michael J.; Morgan, Gary J.; Fogarty, Gerald B; Veivers, David; Rees, Guy; Rose, Barbara

doi:10.1093/annonc/mds205

Cited by 54 publications

(63 citation statements)

References 18 publications

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“…These covariates were chosen for inclusion in the multivariate model on the basis of the results of a previous analysis. 25 Age was fitted as a continuous covariate and the appropriateness of this was evaluated. All other covariates were fitted as categorical variables.…”

Section: Statistical Analysesmentioning

confidence: 99%

Human papillomavirus, smoking status and outcomes in tonsillar squamous cell carcinoma

Hong

Martin

Chatfield

et al. 2012

Intl Journal of Cancer

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It is now clear that the two separate entitles of tonsillar cancer, HPV induced and non-HPV induced (smoking induced), have significantly different presenting stage and outcomes. A significant proportion of patients with human papillomavirus positive tonsillar cancer have had exposure to smoking. We examined the combined effect of human papillomavirus and smoking on the outcomes and determined whether smoking can modify the beneficial effect of human papillomavirus. A total of 403 patients from nine centers were followed up for recurrence or death for a median of 38 months. Determinants of the rate of loco-regional recurrence, death from tonsillar cancer and overall survival were modeled using Cox regression. Smoking status was a significant predictor of overall survival (p 5 0.04). There were nonstatistically significant trends favoring never smokers for loco-regional recurrence and disease specific survival. In addition, there was no statistically significant interactions between smoking and human papillomavirus (p-values for the interaction were 0.26 for loco-regional recurrence, 0.97 for disease specific survival and 0.73 for overall survival). The effect of smoking on loco-regional recurrence and disease specific survival outcomes was not statistically significant, nor was there significant evidence that the effect of smoking status on these outcomes was modified by HPV status. Irrespective of HPV status, however, smokers did have poorer overall survival than never-smokers, presumably due to effects of smoking that are unrelated to the primary cancer.

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Section: Statistical Analysesmentioning

confidence: 99%

Human papillomavirus, smoking status and outcomes in tonsillar squamous cell carcinoma

Hong

Martin

Chatfield

et al. 2012

Intl Journal of Cancer

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“…5 Furthermore, patients with HPV positive OSCC have a better prognosis, despite the fact that they often present with higher grade tumors and more advanced nodal disease. 6 The reason for better outcomes seen in patients with HPV positive OSCC is not fully understood and could be related to intrinsic radiosensitivity of the HPV positive OSCC. 7,8 Various OSCC cell lines have been established but only a few HPV positive OSCC cell lines are available for in vitro study.…”

Section: Introductionmentioning

confidence: 99%

In vitro3-dimensional tumor model for radiosensitivity of HPV positive OSCC cell lines

Zhang

Rose

Lee

et al. 2015

Cancer Biology & Therapy

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The incidence of oropharyngeal squamous cell carcinoma (OSCC) is increasing due to the rising prevalence of human papillomavirus (HPV) positive OSCC. HPV positive OSCC is associated with better outcomes than HPV negative OSCC. Our aim was to explore the possibility that this favorable prognosis is due to the enhanced radiosensitivity of HPV positive OSCC. HPV positive OSCC cell lines were generated from the primary OSCCs of 2 patients, and corresponding HPV positive cell lines generated from nodal metastases following xenografting in nude mice. Monolayer and 3 dimensional (3D) culture techniques were used to compare the radiosensitivity of HPV positive lines with that of 2 HPV negative OSCC lines. Clonogenic and protein assays were used to measure survival post radiation. Radiation induced cell cycle changes were studied using flow cytometry. In both monolayer and 3D culture, HPV positive cells exhibited a heterogeneous appearance whereas HPV negative cells tended to be homogeneous. After irradiation, HPV positive cells had a lower survival in clonogenic assays and lower total protein levels in 3D cultures than HPV negative cells. Irradiated HPV positive cells showed a high proportion of cells in G1/S phase, increased apoptosis, an increased proliferation rate, and an inability to form 3D tumor clumps. In conclusion, HPV positive OSCC cells are more radiosensitive than HPV negative OSCC cells in vitro, supporting a more radiosensitive nature of HPV positive OSCC.

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“…[4] [5] Similarly Chaturvedi et al also studied exclusively in Oropharyngeal squamous cell carcinoma and the prevalence of HPV in their study was 44.1%. [6] On the other hand Benjamin et al had low prevalence of HPV in their study and the reason for this may be because apart from Oral cavity and Oropharynx he also selected patients of SCC of Esophagus, Hypopharynx and Pyriform sinus where prevalence of HPV is low.…”

Section: Discussionmentioning

confidence: 99%

Association of uncommon sexual practices with Human Papilloma Virus in Head and Neck Squamous Cell Carcinoma

Beniwal¹

2017

JMSCR

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Human papillomavirus modifies the prognostic significance of T stage and possibly N stage in tonsillar cancer

Abstract: This study suggests that the prognostic significance of the conventional staging system in tonsillar cancer is modified by HPV.

Cited by 54 publications

References 18 publications

Human papillomavirus, smoking status and outcomes in tonsillar squamous cell carcinoma

Human papillomavirus, smoking status and outcomes in tonsillar squamous cell carcinoma

In vitro3-dimensional tumor model for radiosensitivity of HPV positive OSCC cell lines

Association of uncommon sexual practices with Human Papilloma Virus in Head and Neck Squamous Cell Carcinoma

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