Aureivirga marina gen. nov., sp. nov., a marine bacterium isolated from the Mediterranean sponge Axinella verrucosa

Abstract

Two bacterial strains, VI.14 and VIII.04T, were isolated from the Mediterranean sponge Axinella verrucosa collected off the Israeli coast near Sdot Yam. The non-motile, aerobic, Gram-negative isolates were oxidase-negative and catalase-positive, and formed golden-brown colonies on marine agar 2216. The pigment was neither diffusible nor flexirubin-like. Strain VIII.04T grew at 15–37 °C, at pH 6.0–9.0, in the presence of 20–50 g NaCl l−1 and 20–80 g sea salts l−1, The spectrum was narrower for strain VI.14, with growth at pH 7.0–8.0. and in the presence of 30–50 g NaCl l−1 and 30–70 g sea salts l−1. The predominant fatty acid (>50 %) in both strains was iso-C15 : 0, and the major respiratory quinone was MK-6. The DNA G+C content was 30.7 and 31.1 mol% for VIII.04T and VI.14, respectively. Results from 16S rRNA sequence similarity and phylogenetic analyses indicated that both strains are closely related to members of the family Flavobacteriaceae within the phylum Bacteroidetes , with as much as 91.7 % 16S rRNA sequence similarity. On the basis of data from the polyphasic analysis, we suggest that the strains represent a novel species in a new genus within the family Flavobacteriaceae , for which the name Aureivirga marina gen. nov., sp. nov. is proposed. Strain VIII.04T ( = ATCC BAA-2394T = LMG 26721T) is the type strain of Aureivirga marina.

Diversity and antibacterial activity of bacteria cultured from MediterraneanAxinellaspp. sponges

“…Four isolates of the phylum Bacteroidetes were subsequently characterized in detail, which lead to the establishment of three new genera (Haber et al . ,b,c).…”

“…Four of these seven isolates have been chosen for a detailed analysis and were confirmed to belong to three new genera of the phylum Bacteroidetes (Haber et al . ,b,c). The comparatively low number (14) of different genera obtained from A. cannabina might be skewed due to the availability of only a single specimen from which isolates were cultured.…”

Description of Lutimonas halocynthiae sp. nov., isolated from a golden sea squirt (Halocynthia aurantium), reclassification of Aestuariicola saemankumensis as Lutimonas saemankumensis comb. nov. and emended description of the genus Lutimonas

“…The type strains of L. vermicola and A. saemankumensis were isolated from a marine polychaete inhabiting a tidal flat of Donggum island and a tidal flat sediment of Saemangum, respectively, on the Yellow Sea in South Korea. The genera Lutimonas and Aestuariicola have been found to be phylogenetically closely related, the type strains of the type species showing 99.2 % 16S rRNA gene sequence similarity (Park et al , 2009; Jung et al , 2012; Haber et al , 2013). The aim of the present work was to evaluate the taxonomic status of the genera Lutimonas and Aestuariicola as well as to determine the exact taxonomic position of strain RSS3-C1 T by using a polyphasic characterization.…”

Aureivirga callyspongiae sp. nov., Isolated from Marine Sponge Callyspongia elegans

“…One of the isolates, strain CE67 T , was selected for a later taxonomic experiment. Comparative 16s rRNA gene sequence analysis indicated that the novel CE67 T strain was phylogenetically most closely related to members of the genera Aureivirga [3], Namhaeicola [4], Actibacter [5], and Lutimonas [6,7]. The genus Aureivirga in the phylum Bacteroidetes is a new member of the family Flavobacteriaceae, and was first reported by Haber et al in 2013 [3].…”