22.3 Matrix-induced Autologous Chondrocyte Implantation (MACI®): Biological and Histological Assessment.

Zheng, Ming; Willers, Craig; Kirilak, Lyn; Yates, Piers; Wood, David; Shimmin, A

doi:10.1016/s1063-4584(07)61326-0

Cited by 4 publications

(3 citation statements)

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“…[3][4][5] If multiple osteochondral plugs are used to fill a large defect site, integration between the plugs is also poor. Matrix-induced autologous chondrocyte implantation (MACI) was the first FDA approved cell and scaffoldbased cartilage repair therapy, 6,7 and in this process in vitro expanded ACh are seeded onto a collagen membrane which is implanted into the cartilage defect site. As the implanted ACh are not trapped in lacune, they can remodel and form new cartilage tissue.…”

Section: Introductionmentioning

confidence: 99%

Collagenase treatment appears to improve cartilage tissue integration but damage to collagen networks is likely permanent

et al. 2022

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When repairing cartilage defects a major challenge is achieving high-quality integration between the repair tissue and adjacent native cartilage. Matrix-rich cartilage is not easily remodeled, motivating several studies to trial enzyme treatment of the tissue interface to facilitate remodeling and integration. Studying and optimizing such processes is tedious, as well as potentially expensive, and thus simpler models are needed to evaluate the merits of enzyme treatment on cartilage tissue integration. Herein, we used engineered cartilage microtissues formed from bone marrow-derived stromal cells (BMSC) or expanded articular chondrocytes (ACh) to study the impact of enzyme treatment on cartilage tissue integration and matrix remodeling. A 5-min treatment with collagenase appeared to improve cartilage microtissue integration, while up to 48 h treatment with hyaluronidase did not. Alcian blue and anti-collagen II staining suggested that collagenase treatment did facilitate near seamless integration of cartilage microtissues. Microtissue sections were stained with Picrosirius red and characterized using polarized light microscopy, revealing that individual microtissues contained a collagen network organized in concentric shells. While collagenase treatment appeared to improve tissue integration, assessment of the collagen fibers with polarized light indicated that enzymatically damaged networks were not remodeled nor restored during subsequent culture. This model and these data paradoxically suggest that collagen network disruption is required to improve cartilage tissue integration, but that the disrupted collagen networks are unlikely to subsequently be restored. Future studies should attempt to limit collagen network disruption to the surface of the cartilage, and we recommend using Picrosirius red staining and polarized light to assess the quality of matrix remodeling and integration.

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Section: Introductionmentioning

confidence: 99%

Collagenase treatment appears to improve cartilage tissue integration but damage to collagen networks is likely permanent

et al. 2022

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“…The above concerns led to the development of Matrix-Carried Autologous Chondrocye Implantation (MACI; Verigen, Leverkusen, Germany) that has a similar porcine-derived type I/III collagen membrane (Zheng et al, 2007). The membrane can be secured to the cartilage defect with fibrin glue and does not require additional cover, allowing the procedure to be performed faster and with less extensive exposure (Bartlett et al, 2005).…”

Section: Matrix-carried Autologous Chondrocyte Implantationmentioning

confidence: 99%

Operative Treatment of Knee Cartilage Injuries: A Review of the Current Literature on Non-Cell-Based and Cell-Based Therapies

Jaiswal

2011

BJMMR

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Aims: Cartilage is frequently damaged through injury and disease but shows little or no capacity for repair. Injuries that extend to the subchondral level show some capacity for repair due to the release of bone marrow derived mesenchymal stem cells. Focal articular cartilage defects are challenging clinical problems that may progress to more generalised lesions. We reviewed the literature to analyse the results of available noncell-based and cell-based strategies for the repair of articular cartilage defects in the knee.

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“…The collagen membrane was utilized as substrate for the so-called matrix-associated autologous chondrocyte transplantation (MACT, MACI®). Since the introduction of the MACI® technique in 1998, more than 3000 patients have been treated across Europe and Australia 1 . This simple surgical technique obviates periosteal harvest, is generally suture free, is less invasive than traditional treatment methods and allows an early mobilization of the joint.…”

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confidence: 99%

Extended Abstracts

2009

Cartilage

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The soccer athlete has a greater incidence of overuse and acute knee injuries and as a consequence is at most risk for chondropenia and osteoarthritis. Chondropenia conceptually defines the complex nature of the multivariable processes over time including acute and chronic injury, modulators and the aging process. The role of the Sports Medicine team is to prevent injury, restore the joint and ultimately return the athlete to sport while preventing Osteoarthritis. The clinical consequences of full thickness Articular Cartilage defects are pain, swelling, mechanical symptoms athletic and functional disability and osteoarthitis. In the soccer athlete it is articular cartilage that confers the highest levels of performance. It is the fact that any partial or full thickness loss results in loss of day to day resilience and a spectrum of soreness, stiffness, pain and swelling and most importantly. These injuries may be career- endingIn this group of high demand participants any increase in activity and loading beyond the articular cartilage threshold for injury, results in a clinical overuse response with the potential negative adaptive consequences of chondropenia and an increased risk of developing osteoarthritis. Lesions to articular cartilage are common acutely but are associated with ACL injuries and long-term chondropenia and Osteoarthitis. The challenge of ACL injury reduction, articular cartilage repair and regeneration and osteoarthitis prevention in the soccer athlete continues to be significant despite significant recent advances. The purpose of this lecture is to present the current concepts with respect to the Athlete's Articular Cartilage the histological, biochemical and clinical implications and contemporary treatments from a historical and an evolutionary prospective as applied during or after their competitive years.

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22.3 Matrix-induced Autologous Chondrocyte Implantation (MACI®): Biological and Histological Assessment.

Cited by 4 publications

References 0 publications

Collagenase treatment appears to improve cartilage tissue integration but damage to collagen networks is likely permanent

Collagenase treatment appears to improve cartilage tissue integration but damage to collagen networks is likely permanent

Operative Treatment of Knee Cartilage Injuries: A Review of the Current Literature on Non-Cell-Based and Cell-Based Therapies

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