“…Table eggs are very susceptible to fungal contamination under unsuitable storage conditions, such as increased humidity and absence of air circulation [30]. Fungal contamination of chicken eggs has been described in several studies [31][32][33]. Regecová et al [26] reported the occurrence of microscopic filamentous fungi of Penicillium spp., Aspergillus spp., Cladosporium spp., and Alternaria alternate group in chicken eggs.…”
Penicillium species belong to main causative agents of food spoilage leading to significant economic losses and potential health risk for consumers. These fungi have been isolated from various food matrices, including table eggs. In this study, both conventional Polymerase Chain Reaction (PCR) and Polymerase Chain Reaction-Internal Transcribed Spacer-Restriction Fragment Length Polymorphism (PCR-ITS-RFLP) methods were used for species identification of Penicillium (P.) spp. isolated from the eggshells of moldy chicken eggs. Seven restriction endonucleases (Bsp1286I, XmaI, HaeIII, HinfI, MseI, SfcI, Hpy188I) were applied to create ribosomal restriction patterns of amplified ITS regions. To identify P. verrucosum, P. commune, and P. crustosum with the help of conventional PCR assay, species-specific primer pairs VERF/VERR, COMF/COMR, and CRUF/CRUR were designed on the base of 5.8 subunit-Internal Transcribed Spacer (5.8S-ITS) region. Altogether, 121 strains of microscopic filamentous fungi were isolated by traditional culture mycological examination. After morphological evaluation of both macroscopic and microscopic features, 96 strains were classified in Penicillium spp. Two molecular methods used have confirmed eight isolates as P. verrucosum, 42 isolates as P. commune, and 19 isolates as P. crustosum. Both PCR-ITS-RFLP and conventional PCR assays appear to be suitable alternatives for rapid identification of the above mentioned Penicillium species.
“…Table eggs are very susceptible to fungal contamination under unsuitable storage conditions, such as increased humidity and absence of air circulation [30]. Fungal contamination of chicken eggs has been described in several studies [31][32][33]. Regecová et al [26] reported the occurrence of microscopic filamentous fungi of Penicillium spp., Aspergillus spp., Cladosporium spp., and Alternaria alternate group in chicken eggs.…”
Penicillium species belong to main causative agents of food spoilage leading to significant economic losses and potential health risk for consumers. These fungi have been isolated from various food matrices, including table eggs. In this study, both conventional Polymerase Chain Reaction (PCR) and Polymerase Chain Reaction-Internal Transcribed Spacer-Restriction Fragment Length Polymorphism (PCR-ITS-RFLP) methods were used for species identification of Penicillium (P.) spp. isolated from the eggshells of moldy chicken eggs. Seven restriction endonucleases (Bsp1286I, XmaI, HaeIII, HinfI, MseI, SfcI, Hpy188I) were applied to create ribosomal restriction patterns of amplified ITS regions. To identify P. verrucosum, P. commune, and P. crustosum with the help of conventional PCR assay, species-specific primer pairs VERF/VERR, COMF/COMR, and CRUF/CRUR were designed on the base of 5.8 subunit-Internal Transcribed Spacer (5.8S-ITS) region. Altogether, 121 strains of microscopic filamentous fungi were isolated by traditional culture mycological examination. After morphological evaluation of both macroscopic and microscopic features, 96 strains were classified in Penicillium spp. Two molecular methods used have confirmed eight isolates as P. verrucosum, 42 isolates as P. commune, and 19 isolates as P. crustosum. Both PCR-ITS-RFLP and conventional PCR assays appear to be suitable alternatives for rapid identification of the above mentioned Penicillium species.
“…Nas poedeiras ocorre perda da eficácia nutricional, por menor disponibilidade de proteínas e lipídeos para metabolização, leva à produção de ovos com tamanho reduzido, gemas menores devido a menor deposição de lipídeos, albúmen menos denso com baixa qualidade proteica (Hassan & Ahmad, 2015;Kaneko et al, 2008).…”
Section: Micotoxinas Presentes No Milhounclassified
“…A qualidade da casca dos ovos não é afetada pelas aflatoxinas, entretanto, apresenta maior resistência com aumento da espessura da casca afetando a eclodibilidade, as trocas gasosas entre o embrião e o ambiente são comprometidas o que causa aumento da mortalidade embrionária. Explicadas pelo fato de a aflatoxina ser metabolizada no fígado e depositada na gema dos ovos na forma de aflatoxina M1, assim como o aflatoxicol e até mesmo a aflatoxina B1, também podem ser encontrados na gema, a ingestão das aflatoxinas eleva os casos de aflatoxicose e a mortalidade embrionária no terço final da incubação (Monson et al, 2015;Hassan & Ahmad, 2015).…”
Section: Micotoxinas Presentes No Milhounclassified
Bird breeding is based on the use of grains that require quality for the processing of the rations. Among the possible contaminants that affect the success of the enterprise are mycotoxicoses caused by fungi, from the field to the storage of the ingredients present in the formulations of the diets. The toxins with the greatest impact on poultry farming are aflatoxin B1, ochratoxin A, T-2 toxin and diacetoxiscirpenol (DAS), vomitoxin and deoxynivalenol (DON), zearalenone and fumonisin B1. These compounds cause losses in production, as a result of lower feed intake, mainly for causing lesions in the oral route of the animals, which consequently decreases the weight gain, feed conversion, and meat and egg production, as well as metabolic disorders. Maize consists of a grain with a greater presence of these mycotoxins, being extremely important the monitoring of its qualification in order to offer rations with nutritional and sanitary safety, with success in the business and products of quality and food safety for human consumption.
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