(20R)-Panaxadiol as a Natural Active Component with Anti-Obesity Effects on ob/ob Mice via Modulating the Gut Microbiota

Lv, Yuqian; Zhang, Yining; Feng, Jianshu; Zhao, Tianyu; Zhao, Jingtong; Ge, Yuxiang; Yang, Xuehan; Han, Hao; Zhang, Ming; Chen, Li; Xu, Mingzhu; Guan, Fengying

doi:10.3390/molecules27082502

Cited by 7 publications

(4 citation statements)

References 39 publications

(39 reference statements)

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“…Lachnospiraceae , one of the 16S taxa that was associated with lower stool proline, expresses proline reductase PrdA and has been reported to compete with other microbiota for proline and other amino acids ( Mefferd et al, 2020 ). Muribaculaceae , which was also linked to lower stool proline in our model, is associated with anti-obesity effects and produces butyrate, a short chain fatty acid that attenuates lung inflammation in murine models of allergic airway disease ( Cait et al, 2017 ; Xu et al, 2020 ; Ye et al, 2021 ; Lv et al, 2022 ). However, we did not observe changes in short chain fatty acids measured from cecal tissue in this model ( Supplement Figure 6 ).…”

Section: Discussionmentioning

confidence: 86%

Meta-omics profiling of the gut-lung axis illuminates metabolic networks and host-microbial interactions associated with elevated lung elastance in a murine model of obese allergic asthma

et al. 2023

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Obesity and associated changes to the gut microbiome worsen airway inflammation and hyperresponsiveness in asthma. Obesogenic host-microbial metabolomes have altered production of metabolites that may influence lung function and inflammatory responses in asthma. To understand the interplay of the gut microbiome, metabolism, and host inflammation in obesity-associated asthma, we used a multi-omics approach to profile the gut-lung axis in the setting of allergic airway disease and diet-induced obesity. We evaluated an immunomodulator, nitro-oleic acid (NO2-OA), as a host- and microbial-targeted treatment intervention for obesity-associated allergic asthma. Allergic airway disease was induced using house dust mite and cholera toxin adjuvant in C57BL6/J mice with diet-induced obesity to model obesity-associated asthma. Lung function was measured by flexiVent following a week of NO2-OA treatment and allergen challenge. 16S rRNA gene (from DNA, taxa presence) and 16S rRNA (from RNA, taxa activity) sequencing, metabolomics, and host gene expression were paired with a Treatment-Measured-Response model as a data integration framework for identifying latent/hidden relationships with linear regression among variables identified from high-dimensional meta-omics datasets. Targeting both the host and gut microbiota, NO2-OA attenuated airway inflammation, improved lung elastance, and modified the gut microbiome. Meta-omics data integration and modeling determined that gut-associated inflammation, metabolites, and functionally active gut microbiota were linked to lung function outcomes. Using Treatment-Measured-Response modeling and meta-omics profiling of the gut-lung axis, we uncovered a previously hidden network of interactions between gut levels of amino acid metabolites involved in elastin and collagen synthesis, gut microbiota, NO2-OA, and lung elastance. Further targeted metabolomics analyses revealed that obese mice with allergic airway disease had higher levels of proline and hydroxyproline in the lungs. NO2-OA treatment reduced proline biosynthesis by downregulation of pyrroline-5-carboxylate reductase 1 (PYCR1) expression. These findings are relevant to human disease: adults with mild-moderate asthma and BMI ≥ 25 had higher plasma hydroxyproline levels. Our results suggest that changes to structural proteins in the lung airways and parenchyma may contribute to heightened lung elastance and serve as a potential therapeutic target for obese allergic asthma.

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Section: Discussionmentioning

confidence: 86%

Meta-omics profiling of the gut-lung axis illuminates metabolic networks and host-microbial interactions associated with elevated lung elastance in a murine model of obese allergic asthma

et al. 2023

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“…Furthermore, the conversion of ginseng into PPT under the influence of gut microbiota enhances the activity of beneficial bacteria, including Bacteroides , Eubacterium , Bifidobacterium , and Fusobacterium . Additionally, certain monomers of ginseng, such as panaxadiol and Rk3, have been found to significantly lower the Firmicutes / Bacteroidetes (F/B) ratio in obese mice, , highlighting their potential as therapeutic targets for obesity-related diseases. Notably, G-Rg1 has exhibited significant effects on the gut microbiota of colitis patients, specifically regulating the abundance of Lachnospiraceae , Staphylococcus , Bacteroide , and Ruminococcaceae_UCG_014 .…”

Section: Discussionmentioning

confidence: 99%

Ginsenoside Rg1 Alleviates Ulcerative Colitis in Obese Mice by Regulating the Gut Microbiota–Lipid Metabolism–Th1/Th2/Th17 Cells Axis

Zhong,

Xiao,

Huang

et al. 2023

J. Agric. Food Chem.

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“…Lactobacillus is an essential probiotic that can prevent obesity by altering the gut microbiota in obese mice (Zhang et al, 2022). It has been reported that Lactobacillus can cut down sugars for energy and produce large amounts of lactic acid, which can increase satiety after a meal (Lv et al, 2022). Moreover, Lactobacillus converts glycosides into aglycones to improve their bioavailability and bioactivity by producing extracellular b-glucosidase.…”

Section: Discussionmentioning

confidence: 99%

Soybean isoflavones modulate gut microbiota to benefit the health weight and metabolism

Huang

Zheng

Hui

et al. 2022

Front. Cell. Infect. Microbiol.

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Soybean isoflavones (SIs) are widely found in food and herbal medicines. Although the pharmacological activities of SIs have been widely reported, their effects on the intestinal microecology of normal hosts have received little attention. Five-week-old Kunming (KM) mice were administered SIs (10 mg/kg/day) for 15 days. Food intake, body weight, and digestive enzyme activity were measured. Small intestine microbiota, including lumen-associated bacteria (LAB) and mucosa-associated bacteria (MAB), were analyzed using 16S ribosomal ribonucleic acid (16S rRNA) gene sequencing. Short-chain fatty acids (SCFAs) were analyzed using gas chromatography-mass spectrometry (GC-MS). The results showed that the mice that consuming SIs showed a higher food intake but a lower body weight gain rate than that of normal mice. Sucrase, cellulase, and amylase activities reduced, while protease activity increased after SIs intervention. Moreover, SIs increased the intestinal bacterial diversity in both LAB and MAB of normal mice. The composition of LAB was more sensitive to SIs than those of MAB. Lactobacillus, Adlercreutzia, Coprococcus, Ruminococcus, Butyricicoccus, and Desulfovibrio were the differential bacteria among the LAB of mice treated with SIs. In addition, acetic acid, valeric acid, isobutyric acid, isovaleric acid, and caproic acid decreased, while butyric acid and propionic acid increased in the mice treated with SIs. Taken together, SIs are beneficial for weight control, even in short-term interventions. The specific mechanism is related to regulating the gut microbiota, changing digestive enzyme activities, and further affecting carbohydrate absorption and metabolism.

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(20R)-Panaxadiol as a Natural Active Component with Anti-Obesity Effects on ob/ob Mice via Modulating the Gut Microbiota

Cited by 7 publications

References 39 publications

Meta-omics profiling of the gut-lung axis illuminates metabolic networks and host-microbial interactions associated with elevated lung elastance in a murine model of obese allergic asthma

Meta-omics profiling of the gut-lung axis illuminates metabolic networks and host-microbial interactions associated with elevated lung elastance in a murine model of obese allergic asthma

Ginsenoside Rg1 Alleviates Ulcerative Colitis in Obese Mice by Regulating the Gut Microbiota–Lipid Metabolism–Th1/Th2/Th17 Cells Axis

Soybean isoflavones modulate gut microbiota to benefit the health weight and metabolism

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