203 Non-lesional and lesional lupus skin share inflammatory phenotypes that drive activation of CD16+ DCs

Billi, Allison C.; Ma, Feiyang; Plazyo, Olesya; Wasikowski, Rachael; Gharaee−Kermani, Mehrnaz; Hurst, Amy; Dobry, Craig J.; Tsoi, L.C.; Guðjónsson, Jóhann E.; Kahlenberg, J. Michelle

doi:10.1136/lupus-2021-lupus21century.7

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“…The final data set comprised 39,086 cells, with an average of 2,252 genes and 10,970 transcripts per cell. Cell‐type clustering was performed as previously described (28). Using Uniform Manifold Approximation and Projection to visualize the characteristic keratinocytes markers, we identified 7 subclusters: basal, spinous, supraspinous, granular, follicular, basal inflammatory, and spinous inflammatory (Figure 2A), with each subcluster comprising keratinocytes from lesional or nonlesional skin of lupus patients and skin of healthy controls (Figure 2B).…”

Section: Resultsmentioning

confidence: 99%

Regulation of Photosensitivity by the Hippo Pathway in Lupus Skin

Hile

Coit

et al. 2023

Arthritis & Rheumatology

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Objective. Photosensitivity is one of the most common manifestations of systemic lupus erythematosus (SLE), yet its pathogenesis is not well understood. The normal-appearing epidermis of patients with SLE exhibits increased ultraviolet B (UVB)-driven cell death that persists in cell culture. Here, we investigated the role of epigenetic modification and Hippo signaling in enhanced UVB-induced apoptosis seen in SLE keratinocytes.Methods. We analyzed DNA methylation in cultured keratinocytes from SLE patients compared to keratinocytes from healthy controls (n = 6/group). Protein expression was validated in cultured keratinocytes using immunoblotting and immunofluorescence. An immortalized keratinocyte line overexpressing WWC1 was generated via lentiviral vector. WWC1-driven changes were inhibited using a large tumor suppressor kinase 1/2 (LATS1/2) inhibitor (TRULI) and small interfering RNA (siRNA). The interaction between the Yes-associated protein (YAP) and the transcriptional enhancer associate domain (TEAD) was inhibited by overexpression of an N/TERT cell line expressing a tetracycline-inducible green fluorescent protein-tagged protein that inhibits YAP-TEAD binding (TEADi). Apoptosis was assessed using cleaved caspase 3/7 and TUNEL staining.Results. Hippo signaling was the top differentially methylated pathway in SLE versus control keratinocytes. SLE keratinocytes (n = 6) showed significant hypomethylation (Δβ = −0.153) and thus overexpression of the Hippo regulator WWC1 (P = 0.002). WWC1 overexpression increased LATS1/2 kinase activation, leading to YAP cytoplasmic retention and altered proapoptotic transcription in SLE keratinocytes. Accordingly, UVB-mediated apoptosis in keratinocytes could be enhanced by WWC1 overexpression or YAP-TEAD inhibition, mimicking SLE keratinocytes. Importantly, inhibition of LATS1/2 with either the chemical inhibitor TRULI or siRNA effectively eliminated enhanced UVB-apoptosis in SLE keratinocytes.Conclusion. Our work unravels a novel driver of photosensitivity in SLE: overactive Hippo signaling in SLE keratinocytes restricts YAP transcriptional activity, leading to shifts that promote UVB apoptosis.

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Section: Resultsmentioning

confidence: 99%