2020 update on human coronaviruses: One health, one world

Zhao, Xixi; Ding, Yuecheng; Du, Jing; Fan, Yubo

doi:10.1016/j.medntd.2020.100043

Cited by 26 publications

(27 citation statements)

References 226 publications

(212 reference statements)

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“…This phenomenon has been more frequently described for RNA viruses, mainly among Rhabdoviridae and Picornaviridae family members, and it has been associated with its huge adaptive plasticity [ 53 , 54 ]. From the human health perspective, some of the more remarkable examples of zoonotic RNA viruses able to break interspecies barrier by the assistance of an intermediate host are influenza A virus (IAV) , human immunodeficiency virus (HIV) and several respiratory coronaviruses [ 55 , 56 , 57 ]. Spillover events are also quite often in plant viruses, linked to multiple factors, including ecological conditions, genetic plasticity of virus components, and host factor requirements [ 8 , 58 ].…”

Section: Discussionmentioning

confidence: 99%

Virus Host Jumping Can Be Boosted by Adaptation to a Bridge Plant Species

et al. 2021

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Understanding biological mechanisms that regulate emergence of viral diseases, in particular those events engaging cross-species pathogens spillover, is becoming increasingly important in virology. Species barrier jumping has been extensively studied in animal viruses, and the critical role of a suitable intermediate host in animal viruses-generated human pandemics is highly topical. However, studies on host jumping involving plant viruses have been focused on shifting intra-species, leaving aside the putative role of “bridge hosts” in facilitating interspecies crossing. Here, we take advantage of several VPg mutants, derived from a chimeric construct of the potyvirus Plum pox virus (PPV), analyzing its differential behaviour in three herbaceous species. Our results showed that two VPg mutations in a Nicotiana clevelandii-adapted virus, emerged during adaptation to the bridge-host Arabidopsis thaliana, drastically prompted partial adaptation to Chenopodium foetidum. Although both changes are expected to facilitate productive interactions with eIF(iso)4E, polymorphims detected in PPV VPg and the three eIF(iso)4E studied, extrapolated to a recent VPg:eIF4E structural model, suggested that two adaptation ways can be operating. Remarkably, we found that VPg mutations driving host-range expansion in two non-related species, not only are not associated with cost trade-off constraints in the original host, but also improve fitness on it.

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Section: Discussionmentioning

confidence: 99%

Virus Host Jumping Can Be Boosted by Adaptation to a Bridge Plant Species

et al. 2021

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“…There are seven human coronaviruses (HCoV), highly diverse and causing respiratory diseases with mild to severe outcomes [22]. Currently, no specific antiviral drugs to treat HCoV infection are available, although hydroxychloroquine (HCQ) has been suggested as appropriate [23].…”

Section: Methodsmentioning

confidence: 99%

SPR-Based Kinetic Analysis of the Early Stages of Infection in Cells Infected with Human Coronavirus and Treated with Hydroxychloroquine

et al. 2021

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Cell-based assays are a valuable tool for examination of virus–host cell interactions and drug discovery processes, allowing for a more physiological setting compared to biochemical assays. Despite the fact that cell-based SPR assays are label-free and thus provide all the associated benefits, they have never been used to study viral growth kinetics and to predict drug antiviral response in cells. In this study, we prove the concept that the cell-based SPR assay can be applied in the kinetic analysis of the early stages of viral infection of cells and the antiviral drug activity in the infected cells. For this purpose, cells immobilized on the SPR slides were infected with human coronavirus HCov-229E and treated with hydroxychloroquine. The SPR response was measured at different time intervals within the early stages of infection. Methyl Thiazolyl Tetrazolium (MTT) assay was used to provide the reference data. We found that the results of the SPR and MTT assays were consistent, and SPR is a reliable tool in investigating virus–host cell interaction and the mechanism of action of viral inhibitors. SPR assay was more sensitive and accurate in the first hours of infection within the first replication cycle, whereas the MTT assay was not so effective. After the second replication cycle, noise was generated by the destruction of the cell layer and by the remnants of dead cells, and masks useful SPR signals.

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“…Amino acids encoded by primers used for PCR amplification of the Cf-eIF(iso)4E sequence are italicized. Asterisks in the numbering of the partial Cf-eIF(iso)4E sequence indicate that for the count, missing amino acids were replaced by the equivalent ones from the full-length sequence of the Chenopodium quinoa eIF(iso)4E protein (LOC110697254 and several respiratory coronaviruses [55][56][57]. Spillover events are also quite often in plant viruses, linked to multiple factors, including ecological conditions, genetic plasticity of virus components, and host factor requirements [8,58].…”

Section: Sequence Heterogeneities Between Nicotiana Clevelandii Arabmentioning

confidence: 99%

Virus Host Jumping Can Be Boosted by Adaptation to a Bridge Plant Species

Martínez-Turiño

Calvo

Bedoya

et al. 2021

Preprint

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Understanding biological mechanisms that regulate emergence of viral diseases, in particular those events engaging cross-species pathogens spillover, are becoming increasingly important in Virology. Species barrier jumping has been extensively studied in animal viruses, and the critical role of a suitable intermediate host in animal viruses-generated human pandemics is highly topical. However, studies on host jumping involving plant viruses have been focused on shifting intra-species, leaving aside the putative role of “bridge hosts” in facilitating interspecies crossing. Here, we take advantage of several VPg mutants, derived from a chimeric construct of the potyvirus Plum pox virus (PPV), analysing its differential behaviour in three herbaceous species. Our results showed that two VPg mutations in a Nicotiana clevelandii-adapted virus, emerged during adaptation to the bridge-host Arabidopsis thaliana, drastically prompted partial adaptation to Chenopodium foetidum. Although, both changes are expected to facilitate productive interactions with eIF(iso)4E, polymorphims detected in PPV VPg and the three eIF(iso)4E studied, extrapolated to a recent VPg:eIF4E structural model, suggested that two adaptation ways can be operating. Remarkably, we found that VPg mutations driving host-range expansion in two non-related species, not only are not associated with cost trade-off constraints in the original host, but also improve fitness on it.

show abstract

2020 update on human coronaviruses: One health, one world

Cited by 26 publications

References 226 publications

Virus Host Jumping Can Be Boosted by Adaptation to a Bridge Plant Species

Virus Host Jumping Can Be Boosted by Adaptation to a Bridge Plant Species

SPR-Based Kinetic Analysis of the Early Stages of Infection in Cells Infected with Human Coronavirus and Treated with Hydroxychloroquine

Virus Host Jumping Can Be Boosted by Adaptation to a Bridge Plant Species

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