2020–2021 Florida Citrus Production Guide: Planting New Citrus Groves in Florida in the Era of Citrus Greening

Singerman, Ariel; Burani-Arouca, Marina; Futch, Stephen H.

doi:10.32473/edis-cg099-2020

Cited by 2 publications

(2 citation statements)

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“…However, these strategies may be economically and environmentally unsustainable and inefficient for the management of HLB, whose incidence continues to increase, and it is currently estimated to be present in more than 20% of symptomatic trees in the citrus belt of São Paulo and Minas Gerais ( Fundecitrus, 2020 ). In Florida, United States, the citrus industry relies on the production of more than 80% of the orchards that are Las-infected ( Singerman and Useche, 2016 ). Leaves from affected trees show typical HLB symptoms such as asymmetrical chlorosis called blotchy mottle, enlarged veins, and intense canopy defoliation ( Bové, 2006 ).…”

Section: Introductionmentioning

confidence: 99%

Early Population Dynamics of “Candidatus Liberibacter asiaticus” in Susceptible and Resistant Genotypes After Inoculation With Infected Diaphorina citri Feeding on Young Shoots

et al. 2021

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Huanglongbing is a highly destructive citrus disease associated with “Candidatus Liberibacter asiaticus” (Las), a phloem−limited and non-culturable bacterium, naturally transmitted by the psyllid Diaphorina citri. Although diverse approaches have been used to understand the molecular mechanisms involved in the pathogen–host interaction, such approaches have focused on already infected and/or symptomatic plants, missing early events in the initial days post-inoculation. This study aimed to identify the time course of Las multiplication and whole-plant colonization immediately following inoculation by infected psyllids feeding for 2 days. Thus, the experimental approach was to track Las titers after psyllid inoculation in new shoots (NS) of Citrus × sinensis (susceptible), Murraya paniculata (partially resistant), and Bergera koenigii (fully resistant). Soon after psyllid removal, Las titers dropped until the 10–12th days in all three species. Following this, Las titers increased exponentially only in C. × sinensis and M. paniculata, indicating active bacterial multiplication. In C. × sinensis, Las reached a stationary phase at ∼5 log Las cells/g of tissue from the 40th day onward, while in M. paniculata, Las increased at a lower rate of up to ∼3 log Las cells/g of tissue between the 40th and 60th days, decreasing gradually thereafter and becoming undetectable from the 160th day onward. In B. koenigii, Las titers decreased from the start and remained undetectable. In C. × sinensis, an average of 2.6 log of Las cells/g of tissue was necessary for Las to move out of 50% of the NS in 23.6 days and to colonize the rest of the plant, causing a successful infection. Conversely, the probability of Las moving out of the NS remained below 50% in M. paniculata and zero in B. koenigii. To our knowledge, this is the first study on Las dynamics and whole-plant colonization during the earliest stages of infection. Identification of critical time-points for either successful multiplication or Las resistance may help to elucidate initial events of Las–host interactions that may be missed due to longer sampling intervals and at later stages of infection.

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Section: Introductionmentioning

confidence: 99%

Early Population Dynamics of “Candidatus Liberibacter asiaticus” in Susceptible and Resistant Genotypes After Inoculation With Infected Diaphorina citri Feeding on Young Shoots

et al. 2021

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“…The experiments were conducted in the fall of 2015, at the same field site described above and in Tiwari and Stelinski (2013) . The precise C Las infection rate in this citrus grove was unknown but was likely above 80% as it is typical in central Florida ( Singerman and Useche 2016 ). Plots were 5 × 5 tree squares, separated by at least one buffer row.…”

Section: Methodsmentioning

confidence: 97%

Progress Toward an Attract-and-Kill Device for Asian Citrus Psyllid (Hemiptera: Liviidae) Using Volatile Signatures of Citrus Infected With Huanglongbing as the Attractant

Martini

Hoyte

Mafra‐Neto

et al. 2020

Journal of Insect Science

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Asian citrus psyllid, Diaphorina citri (Kuwayama), preferentially orient toward citrus hosts infected with the phytopathogenic bacterium, Candidatus liberibacter asiaticus (CLas) the agent of citrus greening (Huanglongbing, HLB), compared to uninfected counterparts. We investigated whether this preference for the odors of infected plants could be useful for the development of an attract-and-kill (AK) device for D. citri. Twenty-nine blends of volatile organic compounds derived from the odor of citrus infected with CLas were tested in laboratory olfactometer tests, and two blends were also assessed under field conditions. A seven component blend of tricosane: geranial: methyl salicylate: geranyl acetone: linalool: phenylacetaldehyde: (E)-β-ocimene in a 0.40: 0.06: 0.08: 0.29: 0.08: 0.06: 0.03 ratio released from a proprietary slow-release matrix attracted twice more D. citri to yellow sticky traps compared with blank control traps. The attractive blend was subsequently co-formulated with spinosad insecticide into a slow-release matrix to create a prototype AK formulation against D. citri. This formulation effectively reduced the population density of D. citri up to 84% as measured with tap counts when deployed at a density of eight 2.5 g dollops per tree as compared with untreated controls in small plot field trials conducted in citrus orchards. Psyllid populations were not statistically affected at a deployment rate of four dollops per tree. Our results indicate that an AK formulation incorporating spinosad and a volatile blend signature of citrus greening into a slow-release matrix may be useful to suppress D. citri populations.

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2020–2021 Florida Citrus Production Guide: Planting New Citrus Groves in Florida in the Era of Citrus Greening

Cited by 2 publications

References 0 publications

Early Population Dynamics of “Candidatus Liberibacter asiaticus” in Susceptible and Resistant Genotypes After Inoculation With Infected Diaphorina citri Feeding on Young Shoots

Early Population Dynamics of “Candidatus Liberibacter asiaticus” in Susceptible and Resistant Genotypes After Inoculation With Infected Diaphorina citri Feeding on Young Shoots

Progress Toward an Attract-and-Kill Device for Asian Citrus Psyllid (Hemiptera: Liviidae) Using Volatile Signatures of Citrus Infected With Huanglongbing as the Attractant

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