201 - Binding of Cations to Phosphatidylserine Vesicles

Nir, Shlomo; Newton, C.; Papahadjopoulos, Demetrios

doi:10.1016/0302-4598(87)87012-5

Cited by 131 publications

(67 citation statements)

References 17 publications

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“…This effect was discussed at length in McLaughlin et at. (1971), Nir et al (1978) and Nir (1986). Maes and Cremers (1977) raised criticism regarding the use of the Gouy-Chapman double layer model rather than a simpler mass-action exchange model.…”

Section: Application Of the Adsorption Model To The Experimental Resultsmentioning

confidence: 99%

“…Consequently, ignoring this difference can seriously affect the value of the binding coefficient deduced in Equations [1] to [5]. This has been established in numerous studies (McLaughlin et al 1971;Nir et al 1978Nir et al , 1986Hirsch et al 1989). A notable example which emphasizes the importance of the combination of the GouyChapman equation with the mass action equation is illustrated in the adsorption of organic monovalent cations in excess of the CEC, where according to Equation [6] Y0 < 1.…”

Section: Application Of the Adsorption Model To The Experimental Resultsmentioning

confidence: 99%

“…We calculate analytically the excess concentration of mono and divalent cations in the double layer re: gion above their bulk concentrations (see Nir et al 1978).…”

Section: Theoretical Modelmentioning

confidence: 99%

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Exchange Reactions in the Ca-Mg-Na-Montmorillonite System

Rytwo

Banin

Nir

1996

Clays and clay miner.

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Abstract--The exchange reactions of Na, Ca and Mg on montmorillonite are revisited employing recently developed analytical and theoretical approaches. The fractional adsorption of the cations has been determined by displacing them from the adsorbed state in one step, using a low concentration of an organic cation of large binding affinity. The analysis of displaced and solution cations employed inductively coupled plasma emission spectrometry. An adsorption model was employed in the analysis of the data. The procedure consists of solving the electrostatic Gouy-Chapman equations and calculating adsorbed amounts of the cations as the sum of the cations residing in the double layer region, and the cations chemically bound to the surface, in a closed system. The model also accounts explicitly for cation complexation in solution. Thus the calculations also considered the adsorption of CaC1 + and MgCI +, which eliminated the apparent increase of the cation exchange capacity (CEC) with divalent cation concentration. The model could explain and yield predictions for our measured adsorbed amounts as well as previously published data, in the binary and ternary systems of Ca/Mg/Na, provided that the fraction of surface sites occupied by calcium did not exceed 0.4. For a larger coverage of surface sites by calcium, a fit of the experimental data required an order of magnitude increase in the binding coefficients of the divalent cations in the binary system Ca/Na and in the ternary system Ca/Mg/Na.

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Section: Application Of the Adsorption Model To The Experimental Resultsmentioning

confidence: 99%

Section: Application Of the Adsorption Model To The Experimental Resultsmentioning

confidence: 99%

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Exchange Reactions in the Ca-Mg-Na-Montmorillonite System

Rytwo

Banin

Nir

1996

Clays and clay miner.

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“…In the insert of this figure the Ca 2+ binding to cardiolipin in the lamellar phase is shown to reach a limiting value of 0.35 Ca/cardiolipin at a free Ca 2÷ concentration of 2.5 mM, the highest concentration at which lamellar phase can still be detected. From such experimental data intrinsic Ca-cardiolipin binding constants are difficult to derive due to the complexity of non-specific ion-absorption processes [30]. Using a simple binding model and curve fitting procedures a dissociation constant /(ca of 1.5 + 0.5 mM could be derived from these data.…”

Section: Resultsmentioning

confidence: 99%

Further aspects of the Ca2+-dependent polymorphism of bovine heart cardiolipin

Kruijff

Verkleij

Leunissen‐Bijvelt

et al. 1982

Biochimica et Biophysica Acta (BBA) - Biomembranes

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“…Like monovalent cations, bivalent cations strongly bind to acidic phospholipids [35,31,57,[59][60][61][62]. Cations have also been shown to interact with phosphatidylcholine, a zwitterionic lipid [49, 65,661.…”

Section: Discussionmentioning

confidence: 99%

Effect of pH and Monovalent Cations on the Ionization State of Phosphatidylglycerol in Monolayers

Lakhdar-Ghazal

Tichadou

Tocanne

1983

European Journal of Biochemistry

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Ionization of the acidic phospholipid phosphatidylglycerol has been studied by measuring the surface potential of monomolecular films of the lipid as a function of the aqueous subphase pH and the concentration of monovalent cations (Li, Na, Cs). It is shown that theexperimental data can be interpreted by means of the Gouy-Chapman theory in its simplest formulation, provided an adsorption of cations at the membrane surface is accounted for. This allows us to predict the ionization state of the lipid for given ionic conditions in the subphase. Above pH 4, for subphase ion concentration higher than 10 mM, or for ion concentrations above 0.1 mM at pH 5.6, phosphatidylglycerol is fully deprotonated. Within the limits of our theoretical approach, association constants of the cations to the lipid lie around 0.1-0.6 M-I.With the exception of phosphatidylcholine and phosphatidylethanolamine which are zwitterions over a large pH range (3 -8) [l], phospholipids in biomembranes are acidic substances bearing a net negative charge when they are ionized. Ionization or protonation of these acidic lipids as well as their interactions with cations can have considerable consequences on both membrane structure and functions.From a structural point of view, changes in the pH or the ionic strength of the aqueous phase have been shown to trigger phase transition of acidic phospholipids [2-111. The bivalent cations Mg2+ and Ca2+ can bring about phase separation within mixtures of zwitterionic and acidic phospholipids [12-191. Cations can also modify the dynamic properties of lipids, i.e. their lateral diffusion rate [20], as well as being able to promote morphological changes [21]. As an example, cardiolipin, which normally exists in a lamellar phase in the presence of sodium, adopts the hexagonal H,, phase in the presence of calcium [22, 231. Dipalmitoylglycerophosphoglycerol, which normally stays in a lamellar phase in the presence of sodium, has been shown to exist in an interdigitated phase in the presence of the organic cations choline and acetylcholine [24].From a functional point of view, phospholipids can modulate the activity of membrane enzymes by monitoring, at the membrane surface, a concentration of protons, cations and of any charged metabolites which is quite different from that existing in the bulk [25]. Charges at the membrane surface also contribute to the establishment of transmembrane potentials which are involved in energy-linked transmembrane transport processes [26] and which are postulated to monitor the orientation [27 -291 and the activity of certain membrane proteins [28 -341.For all these reasons, it is of great importance to have a full understanding of the ionization process of acidic phospholipids in membrane systems. This would allow a correct prediction of their ionization state and of the surface potential they generate, for given ionic conditions in the aqueous phase. As a first approach, ionization of phosphatidylglycerol was studied in monolayers by measuring changes in film surface pressure again...

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201 - Binding of Cations to Phosphatidylserine Vesicles

Cited by 131 publications

References 17 publications

Exchange Reactions in the Ca-Mg-Na-Montmorillonite System

Exchange Reactions in the Ca-Mg-Na-Montmorillonite System

Further aspects of the Ca2+-dependent polymorphism of bovine heart cardiolipin

Effect of pH and Monovalent Cations on the Ionization State of Phosphatidylglycerol in Monolayers

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