20(S)-Protopanaxatriol promotes the binding of P53 and DNA to regulate the antitumor network via multiomic analysis

Wang, Zhihua; Wu, Wenbo; Guan, Xiangchen; Guo, Shuang; Li, Chaowen; Niu, Ruixue; Gao, Jie; Jiang, Min; Bai, Liping; Leung, Elaine Lai‐Han; Hou, Yuanyuan; Jiang, Zhihong; Bai, Gang

doi:10.1016/j.apsb.2020.01.017

Cited by 19 publications

(12 citation statements)

References 55 publications

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“…The samples were separated using an Agilent 1290 Infinity LC Ultra-High-Performance Liquid Chromatography (UHPLC) HILIC column (25°C; 0.3 mL/min); mobile phase composition A: water + 25 mM ammonium acetate + 25 mM ammonia, B: acetonitrile; gradient elution procedure: 0–0.5 min, 95% B; 0.5–7 min, linear change of B from 95% to 65%; 7–8 min, linear change of B from 65 to 40%; 8–9 min, B maintained at 40%; 9–9.1 min, linear change of B from 40 to 95%; 9.1–12 min, B maintained at 95%; samples were placed in a 4 °C autosampler during the entire analysis [ 19 , 20 ].…”

Section: Methodsmentioning

confidence: 99%

The Differential Metabolomes in Cumulus and Mural Granulosa Cells from Human Preovulatory Follicles

et al. 2021

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This study evaluated the differences in metabolites between cumulus cells (CCs) and mural granulosa cells (MGCs) from human preovulatory follicles to understand the mechanism of oocyte maturation involving CCs and MGCs. CCs and MGCs were collected from women who were undergoing in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) treatment. The differences in morphology were determined by immunofluorescence. The metabolomics of CCs and MGCs was measured by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) followed by quantitative polymerase chain reaction (qPCR) and western blot analysis to further confirm the genes and proteins involved in oocyte maturation. CCs and MGCs were cultured for 48 h in vitro, and the medium was collected for detection of hormone levels. There were minor morphological differences between CCs and MGCs. LC-MS/MS analysis showed that there were differences in 101 metabolites between CCs and MGCs: 7 metabolites were upregulated in CCs, and 94 metabolites were upregulated in MGCs. The metabolites related to cholesterol transport and estradiol production were enriched in CCs, while metabolites related to antiapoptosis were enriched in MGCs. The expression of genes and proteins involved in cholesterol transport (ABCA1, LDLR, and SCARB1) and estradiol production (SULT2B1 and CYP19A1) was significantly higher in CCs, and the expression of genes and proteins involved in antiapoptosis (CRLS1, LPCAT3, and PLA2G4A) was significantly higher in MGCs. The level of estrogen in CCs was significantly higher than that in MGCs, while the progesterone level showed no significant differences. There are differences between the metabolomes of CCs and MGCs. These differences may be involved in the regulation of oocyte maturation.

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Section: Methodsmentioning

confidence: 99%

The Differential Metabolomes in Cumulus and Mural Granulosa Cells from Human Preovulatory Follicles

et al. 2021

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“… 51 Moreover, it was reported that ginsenosides act on the adjacent regions of the p53 DNA‐binding pocket, improve the stable binding of p53 with DNA, and regulate the expression of downstream proteins. 52 Therefore, we speculated that oral administration of Rg3&Rh2 or resveratrol may improve the function of p53 and indirectly increase the biotransformation level of NAD + by enhancing the expression of NMNAT2.…”

Section: Resultsmentioning

confidence: 99%

Improvement of tissue‐specific distribution and biotransformation potential of nicotinamide mononucleotide in combination with ginsenosides or resveratrol

Bai

Yau

Tong

et al. 2022

Pharmacology Res & Perspec

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Decreased Nicotinamide adenine dinucleotide (NAD + ) level has received increasing attention in recent years since it plays a critical role in many diseases and aging. Although some research has proved that supplementing nicotinamide mononucleotide (NMN) could improve the level of NAD + , it is still uncertain whether the NAD + level in specific tissues could be improved in combination with other nutrients. So far, a variety of nutritional supplements have flooded the market, which contains the compositions of NMN coupled with natural products. However, the synergy and transformation process of NMN has not been fully elucidated. In this study, oral administration of NMN (500 mg/kg) combined with resveratrol (50 mg/kg) or ginsenoside Rh2&Rg3 (50 mg/kg) was used to validate the efficacy of appropriate drug combinations in mice. Compared with NMN alone, NMN combined with resveratrol could increase the levels of NAD + in the heart and muscle by about 1.6 times and 1.7 times, respectively, whereas NMN coupled with ginsenoside Rh2&Rg3 could effectively improve the level of NAD + in lung tissue for approximately 2.0 times. Our study may provide new treatment ideas for aging or diseases in cardiopulmonary caused by decreased NAD + levels.

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“…The uppermost bioactive compound was utilized for target prediction using PharmMapper. Integrated biological data interpretation benefited from multi-omics data conversion and the diseaseassociated target database can improve the possibilities for target discovery [22]. Hence, the multi-dimensional protein targets, including the antipyretic-related targets from GeneCards, metabolomics-enriched targets from HEP intervention, and molecular docking targets were integrated.…”

Section: Discussionmentioning

confidence: 99%

Mechanism Assay of Honeysuckle for Heat-Clearing Based on Metabolites and Metabolomics

Wang

Han

et al. 2022

Metabolites

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Nonsteroidal anti-inflammatory drugs (NSAIDs), such as cyclooxygenase (Cox)-1/2 inhibitor, have emerged as potent antipyretics and analgesics. However, few herbs with Cox-1/2 inhibitory activity are commonly used for heat-clearing in China. Although these are known to have antipyretic activity, there is a lack of molecular data supporting their activity. Using the traditional Chinese medicine herb honeysuckle (Hon) as an example, we explored key antipyretic active compounds and their mechanisms of action by assessing their metabolites and metabolomics. Mitogen-activated protein kinase (MAPK) 3 and protein kinase B (AKT) 1 were suggested as key targets regulated primarily by chlorogenic acid (CA) and swertiamarin (SWE). CA and SWE synergistically inhibited the production of interleukin (IL)-1 and IL-6, alleviated generation of prostaglandin E2, and played an antipyretic role equivalent to honeysuckle extract at the same dose contents within 3 h. Collectively, these findings indicated that lipopolysaccharide-induced fever can be countered by CA with SWE synergistically, allowing the substitution of a crude extract of complex composition with active compounds. Our findings demonstrated that, unlike the traditional NSAIDs, the Hon extract showed a remote and indirect mechanism for alleviating fever that depended on the phosphatidylinositol-3-kinase–AKT and MAPK pathways by regulating the principal mediator of inflammation.

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20(S)-Protopanaxatriol promotes the binding of P53 and DNA to regulate the antitumor network via multiomic analysis

Cited by 19 publications

References 55 publications

The Differential Metabolomes in Cumulus and Mural Granulosa Cells from Human Preovulatory Follicles

The Differential Metabolomes in Cumulus and Mural Granulosa Cells from Human Preovulatory Follicles

Improvement of tissue‐specific distribution and biotransformation potential of nicotinamide mononucleotide in combination with ginsenosides or resveratrol

Mechanism Assay of Honeysuckle for Heat-Clearing Based on Metabolites and Metabolomics

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