2-Thiobarbituric Acid as a Reagent for Detecting Milk Fat Oxidation

Patton, Stuart; Kurtz, George W.

doi:10.3168/jds.s0022-0302(51)91763-8

Cited by 200 publications

(71 citation statements)

References 2 publications

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“…1. Antioxidant Activity ---DPPH radical scavenging, 4,5) superoxide dismutase (SOD) scavenging activities (NBT method), 6) and inhibition of lipid peroxidation (TBA method) 7) were evaluated according to the literatures.…”

Section: Methodsmentioning

confidence: 99%

Antioxidant Activity of Hydroxyflavonoids

Furusawa

Tanaka

Ito

et al. 2005

JOURNAL OF HEALTH SCIENCE

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Section: Methodsmentioning

confidence: 99%

Antioxidant Activity of Hydroxyflavonoids

Furusawa

Tanaka

Ito

et al. 2005

JOURNAL OF HEALTH SCIENCE

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“…Lipid peroxidation. The oxidative degradation of unsaturated fatty acids was estimated by a product of lipid peroxidation recognized as malonyl dialdehyde (11)(12)(13). This was assayed by the 2-thiobarbitaric acid technique with final color developtfent in alkaline solution (14).…”

Section: Methodsmentioning

confidence: 99%

Heterogeneity of human platelets

Karpatkin¹,

Strick²

1972

J. Clin. Invest.

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A B S T R A C T Human platelets were separated by density-centrifugation into heavy and light populations. Heavy platelets have an average volume approximately twofold greater than light platelets, and have previously been shown to be young platelets.All 11 enzymes of the Embden-Meyerhof pathway plus the five related enzymes: phosphoglucomutase, glucose-6-P dehydrogenase, 6-P-gluconic dehydrogenase, a-glycerol-P dehydrogenase, and glutathione reductase (TPNH) were examined in cell lysates from total, heavy, and light platelet populations. Apparent Km for individual enzymes were measured in a total platelet population. Empirical Vm.. of the individual enzymes were measured in total, heavy, and light platelet populations. The three apparent rate-limiting enzymes for glycolysis were hexokinase, phosphofructokinase, and glyceraldehyde-3-P dehydrogenase.Heavy platelets contained approximately twofold greater enzyme activity (per gram wet weight) than light platelets for 7 of the 16 enzymes measured: hexokinase, phosphohexoisomerase, phosphofructokinase, glyceraldehyde-3-P dehydrogenase, phosphoglycerokinase, lactic dehydrogenase, and phosphoglucomutase.

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“…Malondialdehyde (MDA), a secondary product of lipid peroxidation, was assayed by reacting the lipid peroxides with thiobarbituric acid (TBA) (Patton & Kurtz 1951). MDA was assayed by the method reported by Ohkawa et al (1979), and the products obtained when 1 mM FeCl 3 was added before boiling were also measured.…”

Section: Measurement Of Lpo Concentrations In Rat Cardiac Musclementioning

confidence: 99%

Lipid peroxidation levels in rat cardiac muscle are affected by age and thyroid status

Shinohara¹,

Mano²,

Nagasaka³

et al. 2000

Journal of Endocrinology

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Free radicals, hydroxyperoxides and H 2 O 2 are all known to damage cell components. This study was designed to compare the concentrations of hydroxyperoxide and free radical scavengers in the cardiac muscles of old rats in the hyper-or hypothyroid condition, to determine whether rates of peroxidation would differ with age, thyroid status, or both.Rats were rendered hyper-or hypothyroid by administration of -thyroxine or methimazole for 4 weeks. Among the old rats, the lipid peroxide (LPO) concentrations, measured as thiobarbituric acid (TBA) reactants, were significantly greater in the hyperthyroid than in the euthyroid state and the LPO concentrations measured as TBA+Fe 3+ reactants, which may be precursors of LPO, were significantly greater in the hyperthyroid state, whereas in young rats, the LPO concentrations measured by TBA or TBA+Fe 3+ methods did not differ significantly in the hyperthyroid state. In the euthyroid state, the concentration of LPO measured as TBA+Fe 3+ reactants was significantly reduced with age. Xanthine oxidase (XOD) activity also was markedly increased with age, being more pronounced in the hyperthyroid than in the euthyroid state. The Mn and Cu/Zn superoxide dismutase activities were greater in the hyperthyroid than in the euthyroid state. Glutathione peroxidase activity decreased with age in the euthyroid and, particularly, in the hyperthyroid state. Catalase activity was not affected in the old rats. Concentrations of -tocopherol in the old rats were high in the hyperthyroid state and low in the hypothyroid state, whereas the levels of -and -tocopherols in these rats were unchanged in both conditions as compared with the euthyroid state findings.Data suggest that the site of free radical generation differs in older rats, with additional shifts in the location of intracellular lipid peroxidation being noted during hyperthyroidism. Thus, as rats age, the reduction of the free radical scavenger system and the increase in LPO and XOD activities might induce myocardial dysfunction.

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2-Thiobarbituric Acid as a Reagent for Detecting Milk Fat Oxidation

Cited by 200 publications

References 2 publications

Antioxidant Activity of Hydroxyflavonoids

Antioxidant Activity of Hydroxyflavonoids

Heterogeneity of human platelets

Lipid peroxidation levels in rat cardiac muscle are affected by age and thyroid status

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