2 Serotyping of Escherichia coli

Ørskov, Frits; Ørskov, Ida

doi:10.1016/s0580-9517(08)70447-1

Cited by 466 publications

(339 citation statements)

References 162 publications

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“…Serotyping and PCR typing of flagellar (fliC) genes of E. coli -Serotyping of E. coli O and H-antigens was performed as described previously (Orskov & Orskov 1984), at the National Reference Laboratory for Escherichia coli, Germany, by L Beutin. Determination of flagellar (fliC) genotypes was performed by PCR followed by analysis of HhaI digested fliC specific PCR products as described previously (Beutin et al 2004).…”

Section: Methodsmentioning

confidence: 99%

Prevalence of diarrheagenic Escherichia coli in children with diarrhea in Salvador, Bahia, Brazil

Franzolin

Alves

Keller

et al. 2005

Mem. Inst. Oswaldo Cruz

105

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Section: Methodsmentioning

confidence: 99%

Prevalence of diarrheagenic Escherichia coli in children with diarrhea in Salvador, Bahia, Brazil

Franzolin

Alves

Keller

et al. 2005

Mem. Inst. Oswaldo Cruz

105

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“…This is because the O104 O antigen is identical to the K9 capsular antigen [9]. The K9 antiserum is readily available from SSI Diagnostica, Hillerød, Denmark (ivdorders@ssi.dk) and described on the SSI website [10].…”

Section: Screening For the Outbreak Strainmentioning

confidence: 99%

Characteristics of the enteroaggregative Shiga toxin/verotoxin-producing Escherichia coli O104:H4 strain causing the outbreak of haemolytic uraemic syndrome in Germany, May to June 2011

et al. 2011

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The Escherichia coli strain causing a large outbreak of haemolytic uraemic syndrome and bloody diarrhoea in Germany in May and June 2011 possesses an unusual combination of pathogenic features typical of enteroaggregative E. coli together with the capacity to produce Shiga toxin. Through rapid national and international exchange of information and strains the known occurrence in humans was quickly assessed. We describe simple diagnostic screening tools to detect the outbreak strain in clinical specimens and a novel real-time PCR for its detection in foods.

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“…A common cross-reaction is between serotypes HI and H12 (Ewing, 1986;0rskov & 0rskov, 1984). The MAbs reported here reacted only with H12 and not with H1 flagellin.…”

Section: Discussionmentioning

confidence: 65%

Serotype-specific Monoclonal Antibodies against the H12 Flagellar Antigen of Escherichia coli

Whitfield

Walker²,

Atkinson³

et al. 1988

Microbiology

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The flagellar filaments of morphotype E isolates of Escherichia coli characteristically possess an apparent helically arranged sheath structure, surrounding the central core of the filament. Reexamination of the type strains of H-serotypes belonging to morphotype E showed that all but serotype H34 possessed the expected morphology. Heterogeneity was observed in both the diameter of filaments from individual morphotype E strains and in the M , of individual flagellins. There was no apparent correlation between these two features. Monoclonal antibodies (MAbs) of the IgM class were raised against serotype H12 flagella. In Western immunoblotting and agglutination tests, the MAbs recognized the H12 antigen of six isolates with different 0 : K antigen combinations. The MAbs were H-serotype-specific, with no significant reaction with the H-antigens of other morphotype E strains. The location of the serotype-specific H 12 epitope(s) was studied by immunolabelling with colloidal gold markers. The epitope was surface-exposed and appeared to be helically arranged on the flagellar filament. The pattern of colloidal gold labelling was consistent with the possibility that the H12 serotypespecific epitope resides in the apparent sheath structure.

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2 Serotyping of Escherichia coli

Cited by 466 publications

References 162 publications

Prevalence of diarrheagenic Escherichia coli in children with diarrhea in Salvador, Bahia, Brazil

Prevalence of diarrheagenic Escherichia coli in children with diarrhea in Salvador, Bahia, Brazil

Characteristics of the enteroaggregative Shiga toxin/verotoxin-producing Escherichia coli O104:H4 strain causing the outbreak of haemolytic uraemic syndrome in Germany, May to June 2011

Serotype-specific Monoclonal Antibodies against the H12 Flagellar Antigen of Escherichia coli

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