2-Methoxystypandrone improves in vitro -produced bovine embryo quality through inhibition of IKBKB

Mesalam, Ayman; Khan, Imran; Lee, Kyeong-Lim; Song, Seok-Hwan; Chowdhury, M.M.R.; Uddin, Zia; Park, Ki Hun; Kong, Il‐Keun

doi:10.1016/j.theriogenology.2017.05.012

Cited by 31 publications

(29 citation statements)

References 26 publications

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“…Ovaries from Korean native cows (Hanwoo) were collected after slaughter at the local slaughterhouse, placed in physiological saline (0.9% NaCl) at approximately 37.5°C, and transported to the laboratory within 4 hr of slaughter. After washing the ovaries with fresh Dulbecco's phosphate‐buffered saline (D‐PBS), cumulus‐oocyte complexes (COCs) were retrieved, as described by Mesalam et al (). COCs were recovered from follicles (2–8 mm diameter) using an 18‐gauge needle attached to a vacuum pump.…”

Section: Methodsmentioning

confidence: 99%

“…Oocytes were cultured in IVM medium, as described by Mesalam, Khan, et al (). Briefly, COCs were washed three times in IVM medium (Tissue culture media‐199 [TCM‐199]), supplemented with 10% (v/v) foetal bovine serum, 1 μg/ml estradiol‐17β, 10 μg/ml Follicle‐stimulating hormone, 0.6 mM cysteine, and 0.2 mM sodium pyruvate.…”

Section: Methodsmentioning

confidence: 99%

“…In vitro‐matured COCs were fertilized with freeze ‐ thawed bovine sperm, as described by Mesalam et al (). Semen was thawed at 38.5°C for 1 min, and sperm were washed and pelleted in D‐PBS by centrifugation at 1,800 g at room temperature for 5 min.…”

Section: Methodsmentioning

confidence: 99%

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Improved developmental competence in embryos treated with lycopene during in vitro culture system

Chowdhury

Mesalam

Khan

et al. 2018

Molecular Reproduction Devel

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In vitro embryo development remains suboptimal compared to in vivo development due to the challenge from various stressors associated with in vitro culturing of oocytes. When 0.2 μM lycopene was added to oocyte in vitro maturation and embryo culture media, to assess its antioxidant effects on embryo development, we observed a significant (p < 0.05) increase in cleavage and blastocyst development rates compared to the corresponding controls (84.3 ± 0.6% vs. 73.1 ± 1.9% and 41.0 ± 1.4% vs. 33.4 ± 0.7%, respectively). Lycopene also significantly reduced (p < 0.05) intracellular reactive oxygen species concentrations in oocytes and blastocysts, whereas lipid peroxidation and mitochondrial activity increased compared to control conditions. The number of apoptotic nuclei was significantly reduced in the lycopene-treated compared to the control group (1.7 ± 0.1 vs. 4.7 ± 0.3), and the quantity of cells in the trophectoderm (207.1 ± 1.6 vs. 171.3 ± 1.0, respectively) and inner cell mass (41.9 ± 0.4 vs. 36.7 ± 0.4, respectively) was higher following treatment-although the inner cell mass-to-trophectoderm ratio was unchanged (1:3.3 vs. 1:3.4 for lycopene vs. control, respectively). Lycopene supplementation also significantly (p < 0.05) attenuated expression of IKBKB (Inhibitor of nuclear factor kappa B kinase, subunit beta) and reduced Caspase 9 and Caspase 3 protein abundance, while up-regulating GDF9 (Growth and differentiation factor 9), BMP15 (Bone morphogenetic protein 15), SOD2 (Superoxide dismutase 2), NDUFA2 (NADH dehydrogenase), ACADL (Acyl-CoA dehydrogenase, long chain), and ACSL3 (Acyl-CoA synthetase 3, long-chain membrane 3) transcription compared to control. Therefore, co-culturing with lycopene during oocyte maturation improved bovine embryo developmental potential during in vitro culture by improving embryonic resilience to stress.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Improved developmental competence in embryos treated with lycopene during in vitro culture system

Chowdhury

Mesalam

Khan

et al. 2018

Molecular Reproduction Devel

Self Cite

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“…Another employed technique is the analysis of gene expression, especially of enzymes involved in the cellular antioxidant system such as catalase, superoxide dismutase, and glutathione peroxidase, and genes related to apoptotic pathways (ESHTIYAGHI et al, 2016;MESALAM et al, 2017). Moreover, quantification of ROS levels using a fluorescent marker (2′, 7′-dichlorodihydrofluorescein diacetate) allows specific evaluation of the antioxidant ability to reduce intracellular ROS (AMBROGI et al, 2017).…”

Section: Methods For Evaluation Of Antioxidants In Ivepmentioning

confidence: 99%

“…It was observed that 1.0 µM of this substance increases the blastocyst rate and the number of total cells, decreases apoptosis and ROS levels, and influences the expression of genes important for embryonic development (MESALAM et al, 2017).…”

Section: Isolated Substances/compoundsmentioning

confidence: 99%

Use of natural antioxidants in in vitro mammalian embryo production

Santos

Borges

Neta

et al. 2018

SCA

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In vitro embryo production (IVEP) contributes to the quantitative and qualitative aspects of animal reproduction. Nevertheless, inherent technical factors such as oxidative stress can negatively influence the result and this can impair cell metabolism, thus decreasing the rates of in vitro development, and necessitating the supplementation of culture medium with antioxidants. In this context, compounds of natural origin with this property have been highlighted because of the positive results obtained at different stages of IVEP. Thus, this review aims to present the results obtained by using natural antioxidants to minimize the effects of oxidative stress on gametes and embryos. A variety of natural isolated substances and mixtures (essential oils and extracts) have been studied for supplementation of IVEP media, at stages of in vitro maturation, sperm capacitation, in vitro fertilization, and in vitro development of embryos in different mammalian species. Generally, beneficial effects are observed according to the concentration used, thus demonstrating the potential of several natural antioxidants. Therefore, the main challenges in using these compounds as antioxidants during IVEP include proving their efficiency against free radicals and determining the best concentration at each stage. In addition, understanding the mechanisms of action of such antioxidants is crucial to establishing their use in IVEP biotechnology. Key words: Culture medium. Natural bioactive. Oxidative stress. Reproduction. ResumoA produção in vitro de embriões (PIVE) contribui para os aspectos quantitativos e qualitativos da reprodução animal. Contudo, fatores inerentes da técnica, como o estresse oxidativo, podem influenciar negativamente o resultado e isso pode prejudicar o metabolismo celular, diminuindo assim as taxas de desenvolvimento in vitro, e exigindo a suplementação do meio de cultivo com antioxidantes. Neste contexto, os compostos de origem natural com essa propriedade têm se destacado devido aos resultados positivos obtidos em diferentes estágios da PIVE. Assim, esta revisão pretende apresentar os resultados obtidos usando antioxidantes naturais para minimizar os efeitos do estresse oxidativo em gametas e embriões. Uma variedade de substâncias isoladas e de misturas naturais (óleos essenciais e extratos) tem sido estudada para a suplementação de meios de PIVE, nas etapas de maturação in vitro, capacitação espermática, fecundação in vitro e desenvolvimento in vitro de embriões em diferentes espécies de mamíferos. Geralmente, os efeitos benéficos são observados de acordo com a concentração utilizada,

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Effect of nicotinamide supplementation in in vitro fertilization medium on bovine embryo development

Yuan

Mesalam

Song

et al. 2020

Molecular Reproduction Devel

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Increased oxidative stress is one of the main causes of poorly developed embryos in assisted reproductive technologies. Nicotinamide (NAM) has been shown to suppress reactive oxygen species (ROS) production through its potent antioxidative and anti-senescent effects. In the present study, we explored the effects of short-term NAM-treatment (3 and 5 h) during in vitro fertilization (IVF) on the development of bovine embryos. Treatment with 10 mM NAM for 3 h significantly increased the blastocyst formation but extending the treatment to 5 h did not enhance the benefits any further. Immunofluorescence analysis demonstrated that treatment with 10 mM NAM for 3 h decreased the expression of intracellular ROS, 8-oxo-7,8dihydroguanine, caspase-3, and increased the expression of Sirt1, and incorporation of bromodeoxyuridine in one-cell stage embryos. Similarly, the level of H3K56ac significantly increased in the NAM-treated (3 and 5 h) one-cell stage embryos. Contrastingly, the treatment with 10 mM NAM for 5 h increased the caspase-9 level in blastocysts. Collectively, these findings suggest that NAM possesses antioxidant activity and supplementation of IVF medium with 10 mM NAM for 3 h improves the in vitro developmental competence of bovine embryos.

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2-Methoxystypandrone improves in vitro -produced bovine embryo quality through inhibition of IKBKB

Cited by 31 publications

References 26 publications

Improved developmental competence in embryos treated with lycopene during in vitro culture system

Improved developmental competence in embryos treated with lycopene during in vitro culture system

Use of natural antioxidants in in vitro mammalian embryo production

Effect of nicotinamide supplementation in in vitro fertilization medium on bovine embryo development

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