2-Methoxy-2′,4′-dichloro chalcone as an antimicrofoulant against marine bacterial biofilm

Sivakumar, Ponnurengam Malliappan; Prabhawathi, Veluchamy; Doble, Mukesh

doi:10.1016/j.colsurfb.2010.07.037

Cited by 24 publications

(32 citation statements)

References 48 publications

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“…The total carbohydrate present in the biofilm formed by each of the three organisms is estimated as proposed by Sivakumar et al [11]. 50 L of 5% phenol solution is added to 1 mg/ml glucose (standard), vortexed and added to 2 ml of concentrated sulphuric acid.…”

Section: Biofilm Experimentsmentioning

confidence: 99%

“…The polymers were prepared as films cut into 1 cm × 4 cm. These were dipped in 70% ethanol for 30 min and subsequently rinsed three times with sterile PBS [11].…”

Section: Biocompatibility Studymentioning

confidence: 99%

“…The total protein secreted by each of the three organisms is estimated according to the method reported by Sivakumar et al [11]. In brief, 200 L of (1 mg/ml) Bovine Serum Albumin (BSA) is mixed with 1.25 ml of Bradford reagent.…”

Section: Biofilm Experimentsmentioning

confidence: 99%

“…Though there are some reports available on nitration and amination, none of them investigated the anti adhesive characteristic of nitrated and aminated polycarbonate [10]. Hydrophilic materials in addition to their lubricity and biocompatibility, resists microbial adherence thereby preventing the initial stages in the formation of biofilm [11,12].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

In vitro biocompatiblity of modified polycarbonate as a biomaterial

Vani

Thomas

Prabhawathi

et al. 2013

Colloids and Surfaces B: Biointerfaces

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Section: Biofilm Experimentsmentioning

confidence: 99%

“…The polymers were prepared as films cut into 1 cm × 4 cm. These were dipped in 70% ethanol for 30 min and subsequently rinsed three times with sterile PBS [11].…”

Section: Biocompatibility Studymentioning

confidence: 99%

Section: Biofilm Experimentsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

In vitro biocompatiblity of modified polycarbonate as a biomaterial

Vani

Thomas

Prabhawathi

et al. 2013

Colloids and Surfaces B: Biointerfaces

Self Cite

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“…Serine proteases also decrease the hydrophobicity of bacterial cell surfaces and inhibit attachment to hydrophobic substrata (Paul & Jeffrey 1985). Marine paint containing dichlorochalcone damaged cell membranes, reduced surface hydrophobicity, and inhibited the formation of biofilms (Sivakumar et al 2010). However, the molecular targets of these compounds in microfouling organisms have not been revealed.…”

Section: Surface Modifiersmentioning

confidence: 99%

Mini-review: Molecular mechanisms of antifouling compounds

2013

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Various antifouling (AF) coatings have been developed to protect submerged surfaces by deterring the settlement of the colonizing stages of fouling organisms. A review of the literature shows that effective AF compounds with specific targets are ones often considered non-toxic. Such compounds act variously on ion channels, quorum sensing systems, neurotransmitters, production/release of adhesive, and specific enzymes that regulate energy production or primary metabolism. In contrast, AF compounds with general targets may or may not act through toxic mechanisms. These compounds affect a variety of biological activities including algal photosynthesis, energy production, stress responses, genotoxic damage, immunosuppressed protein expression, oxidation, neurotransmission, surface chemistry, the formation of biofilms, and adhesive production/release. Among all the targets, adhesive production/release is the most common, possibly due to a more extensive research effort in this area. Overall, the specific molecular targets and the molecular mechanisms of most AF compounds have not been identified. Thus, the information available is insufficient to draw firm conclusions about the types of molecular targets to be used as sensitive biomarkers for future design and screening of compounds with AF potential. In this review, the relevant advantages and disadvantages of the molecular tools available for studying the molecular targets of AF compounds are highlighted briefly and the molecular mechanisms of the AF compounds, which are largely a source of speculation in the literature, are discussed.

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Papain immobilized polyurethane as an ureteral stent material

Manohar

Doble

2016

J Biomed Mater Res

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Long term use of polyurethane-based ureteral stent is hampered by the development of infection due to the formation of bacterial biofilm and salt deposition. Here papain, is covalently immobilized to polyurethane using glutarldehyde and is investigated as a possible anti-infective ureteral stent material. Fourier transform infrared spectrum confirmed its immobilization. Immobilized enzyme retained 85% of the activity of the free enzyme and about 12% loss of enzyme was observed from the polymer surface in one month. The modified polyurethane showed 8 log reduction in Staphylococcus aureus and 7 log reduction in Escherichia coli live colonies and 3-4 times decrease in the protein and carbohydrate in the biofilms than bare polymer. The amount of calcium and magnesium salts deposited on the polymer surface reduced by 40% after enzyme immobilization. 80% of L6 myoblast cells were viable on this material which indicated that it was noncytotoxic. A linear regression equation with hydrophilicity of the polymer surface and the cell surface hydrophobicity as the two independent variables was able to predict the number of live cells attached on the modified PU. This study indicated the possibility of using such an approach to overcome the problems of ureteral stent associated biofilm and salt encrustation.

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2-Methoxy-2′,4′-dichloro chalcone as an antimicrofoulant against marine bacterial biofilm

Cited by 24 publications

References 48 publications

In vitro biocompatiblity of modified polycarbonate as a biomaterial

In vitro biocompatiblity of modified polycarbonate as a biomaterial

Mini-review: Molecular mechanisms of antifouling compounds

Papain immobilized polyurethane as an ureteral stent material

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