2-Methoxy-1,4-naphthoquinone Induces Metabolic Shifts in <i>Penicillium Digitatum</i> Revealed by High-Dimensional Biological Data

Guo, Meixia; Li, Jun; Xu, Zhen-Lin; Wang, Jie; Li, Taotao; Lei, Hongtao; Duan, Xuewu; Sun, Yuan; Zhang, Xiaoyong; Huang, Riming

doi:10.1021/acs.jafc.0c03396

Cited by 24 publications

(30 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To deeply understand the action mechanism of nanoemulsion from molecular aspects and seek the pathway involved, we used RNA-Seq technology to profile the transcriptome of P. digitatum treated with nanoemulsion, which induced the expression of many stress response genes and the pathway involved to alleviate the adverse effect. Recent research by RNA-seq also observed different gene and pathway responses in P. digitatum cells treated by antifungal agent [( 41 , 42 )].…”

Section: Discussionmentioning

confidence: 99%

Mining RNA-Seq Data to Depict How Penicillium digitatum Shapes Its Transcriptome in Response to Nanoemulsion

et al. 2021

View full text Add to dashboard Cite

Penicillium digitatum is the most severe pathogen that infects citrus fruits during storage. It can cause fruit rot and bring significant economic losses. The continuous use of fungicides has resulted in the emergence of drug-resistant strains. Consequently, there is a need to develop naturally and efficiently antifungal fungicides. Natural antimicrobial agents such as clove oil, cinnamon oil, and thyme oil can be extracted from different plant parts. They exhibited broad-spectrum antimicrobial properties and have great potential in the food industry. Here, we exploit a novel cinnamaldehyde (CA), eugenol (EUG), or carvacrol (CAR) combination antifungal therapy and formulate it into nanoemulsion form to overcome lower solubility and instability of essential oil. In this study, the antifungal activity evaluation and transcriptional profile of Penicillium digitatum exposed to compound nanoemulsion were evaluated. Results showed that compound nanoemulsion had a striking inhibitory effect on P. digitatum in a dose-dependent manner. According to RNA-seq analysis, there were 2,169 differentially expressed genes (DEGs) between control and nanoemulsion-treated samples, including 1,028 downregulated and 1,141 upregulated genes. Gene Ontology (GO) analysis indicated that the DEGs were mainly involved in intracellular organelle parts of cell component: cellular respiration, proton transmembrane transport of biological process, and guanyl nucleotide-binding molecular function. KEGG analysis revealed that metabolic pathway, biosynthesis of secondary metabolites, and glyoxylate and dicarboxylate metabolism were the most highly enriched pathways for these DEGs. Taken together, we can conclude the promising antifungal activity of nanoemulsion with multiple action sites against P. digitatum. These outcomes would deepen our knowledge of the inhibitory mechanism from molecular aspects and exploit naturally, efficiently, and harmlessly antifungal agents in the citrus postharvest industry.

show abstract

Section: Discussionmentioning

confidence: 99%

Mining RNA-Seq Data to Depict How Penicillium digitatum Shapes Its Transcriptome in Response to Nanoemulsion

et al. 2021

View full text Add to dashboard Cite

show abstract

“…SEM observation was carried out on a scanning electron microscope (Zeiss, Oberkochen, Germany) to observe the morphology of P. italicum mycelia. SEM samples were prepared as described previously [ 6 ]. About 1.0 g of mycelia was collected from both treated and control media after 2 days of incubation.…”

Section: Methodsmentioning

confidence: 99%

“…For comparison, the resulting p values were adjusted using Benjamini and Hochberg’s approach for controlling the false discovery rate. Finally, p value < 0.05 and |log2 foldchange| > 1 were set as the criteria used for the screening of differentially expressed genes (DEGs) [ 6 ].…”

Section: Methodsmentioning

confidence: 99%

“…In our previous investigation, 2-methoxy-1, 4-naphthoquinone (MNQ) isolated from Impatiens balsamina Linn, which is a Chinese medicinal plant, was found to significantly inhibit P. italicum by disrupting metabolic processes, especially in energy metabolism and stimulus–response, which are both critical for the growth of the fungus [ 3 ]. Otherwise, MNQ mainly inhibits the growth of P. digitatum by affecting the synthesis of branched-chain amino acids and cell walls [ 6 ]. Based on our previous research, 2-methoxy-1, 4-naphthoquinone (MNQ) is a promising option for the prevention of postharvest decay.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Transcriptomics Integrated with Metabolomics Reveals 2-Methoxy-1, 4-Naphthoquinone-Based Carbon Dots Induced Molecular Shifts in Penicillium italicum

Chen

et al. 2022

JoF

Self Cite

View full text Add to dashboard Cite

Penicillium italicum (P. italicum), a citrus blue mold, is a pathogenic fungus that greatly affects the postharvest quality of citrus fruits with significant economic loss. Our previous research showed that 2-methoxy-1, 4-naphthoquinone (MNQ) inhibited the growth of Penicillium italicum. However, the water dispersibility of MNQ will limit its further application. Herein, we synthesized MNQ-based carbon dots (2−CDs) with better water dispersibility, which showed a potential inhibitory effect on P. italicum (MIC = 2.8 μg/mL) better than that of MNQ (MIC = 5.0 μg/mL). Transcriptomics integrated with metabolomics reveals a total of 601 differentially enriched genes and 270 differentially accumulated metabolites that are co-mapped as disruptive activity on the cell cytoskeleton, glycolysis, and histone methylation. Furthermore, transmission electron microscopy analysis showed normal appearances and intracellular septum of P. italicum after treatment. These findings contribute tofurther understanding of the possible molecular action of 2−CDs.

show abstract

“…The threshold values of '|log2 foldchange| > 1' and 'Padj < 0.05' were adopted to judge differently expressed genes (DEGs). 33 Finally, the enrichment analysis of the DEGs was performed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses.…”

Section: Transcriptome Profilingmentioning

confidence: 99%

Ferulic acid derivatives with piperazine moiety as potential antiviral agents

Yuan

Wang

Liú

et al. 2022

Pest Management Science

View full text Add to dashboard Cite

Background Plant virus diseases are difficult to control and severely threaten the productivity of crops, which leads to huge financial losses. To discover the new antiviral drugs, 34 novel ferulic acid derivatives with piperazine moiety were synthesized, and the antiviral activities were systematically screened as well. Results Bioassay results indicated that most of the target compounds had outstanding antiviral activities against tobacco mosaic virus (TMV) and cucumber mosaic virus (CMV) in vivo. In particular, compound E2 exhibited remarkable curative activities to TMV and CMV with EC50 values of 189.0 and 401.7 μg/mL compared to those for ningnanmycin (387.0, 519.3 μg/mL) and ribavirin (542.1, 721.5 μg/mL). And then the mechanisms of compound E2 were studied by chlorophyll content, differentially expressed proteins and genes tests. Conclusion The excellent antiviral activity of compound E2 was closely associated with the increase in host photosynthesis, which was confirmed by chlorophyll content, differentially expressed proteins and genes assays. Compound E2 can be considered as a lead structure for the discovery of new antiviral agents. © 2022 Society of Chemical Industry.

show abstract

2-Methoxy-1,4-naphthoquinone Induces Metabolic Shifts in Penicillium Digitatum Revealed by High-Dimensional Biological Data

Cited by 24 publications

References 56 publications

Mining RNA-Seq Data to Depict How Penicillium digitatum Shapes Its Transcriptome in Response to Nanoemulsion

Mining RNA-Seq Data to Depict How Penicillium digitatum Shapes Its Transcriptome in Response to Nanoemulsion

Transcriptomics Integrated with Metabolomics Reveals 2-Methoxy-1, 4-Naphthoquinone-Based Carbon Dots Induced Molecular Shifts in Penicillium italicum

Ferulic acid derivatives with piperazine moiety as potential antiviral agents

Contact Info

Product

Resources

About