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            -Decadienoic Acid, a Novel Anti-Oomycete Agent from Coculture of Bacillus subtilis and Trichoderma asperellum

Zhang, Xi-Fen; Li, Qingyu; Wang, Mei; Ma, Siqi; Zheng, Yanfen; Li, Yiqiang; Zhao, Dong‐Lin; Zhang, Chengsheng

doi:10.1128/spectrum.01542-22

Cited by 5 publications

(6 citation statements)

References 72 publications

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“…Interestingly, the most significant improvement was observed in the co-culture of E. amstelodami and B. licheniformis, indicating that several silenced Sun et al, 2022). In recent years, many research reported that Bacillus species was an ideal strain for establishing co-culture systems, on the one hand, Bacillus species, as a representative of biocontrol microorganisms, the co-culture of Bacillus species and other biocontrol microorganisms exhibited stronger inhibition of plant pathogenic microorganisms and promoted plant growth (Karuppiah et al, 2019;Liu H. et al, 2022), on the other hand, the co-culture of Bacillus species and other microorganisms could markedly increase the production of SMs contained multibiological activity (Sun et al, 2022;Zhang X. F. et al, 2022). However, due to the randomness of microbial co-culture as well as the structural complexity of SMs, many potentially promising microbial co-culture combinations remain undiscovered.…”

Section: Discussionmentioning

confidence: 99%

Metabolomic profiles of the liquid state fermentation in co-culture of Eurotium amstelodami and Bacillus licheniformis

Wang

Chen²,

Xin³

et al. 2023

Front. Microbiol.

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As an important source of new drug molecules, secondary metabolites (SMs) produced by microorganisms possess important biological activities, such as antibacterial, anti-inflammatory, and hypoglycemic effects. However, the true potential of microbial synthesis of SMs has not been fully elucidated as the SM gene clusters remain silent under laboratory culture conditions. Herein, we evaluated the inhibitory effect of Staphylococcus aureus by co-culture of Eurotium amstelodami and three Bacillus species, including Bacillus licheniformis, Bacillus subtilis, and Bacillus amyloliquefaciens. In addition, a non-target approach based on ultra-performance liquid chromatography time-of-flight mass spectrometry (UPLC-TOF-MS) was used to detect differences in extracellular and intracellular metabolites. Notably, the co-culture of E. amstelodami and Bacillus spices significantly improved the inhibitory effect against S. aureus, with the combination of E. amstelodami and B. licheniformis showing best performance. Metabolomics data further revealed that the abundant SMs, such as Nummularine B, Lucidenic acid E2, Elatoside G, Aspergillic acid, 4-Hydroxycyclohexylcarboxylic acid, Copaene, and Pipecolic acid were significantly enhanced in co-culture. Intracellularly, the differential metabolites were involved in the metabolism of amino acids, nucleic acids, and glycerophospholipid. Overall, this work demonstrates that the co-culture strategy is beneficial for inducing biosynthesis of active metabolites in E. amstelodami and B. licheniformis.

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Section: Discussionmentioning

confidence: 99%

Metabolomic profiles of the liquid state fermentation in co-culture of Eurotium amstelodami and Bacillus licheniformis

Wang

Chen²,

Xin³

et al. 2023

Front. Microbiol.

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“…In the case of pepper phytophthora blight, the inoculum of Ph. capsici and the diseased soil were prepared according to the method described by Zhang et al (2022) . Briefly, millet was sterilized after being boiled for 30 min and inoculated with Ph.…”

Section: Methodsmentioning

confidence: 99%

“…capsici and the diseased soil were prepared according to the method described by Zhang et al (2022). 23 Briefly, millet was sterilized after being boiled for 30 min and inoculated with Ph. capsici, followed by incubation at 25 °C for 10 days.…”

Section: Methodsmentioning

confidence: 99%

Identification of Lydicamycins as Main Antioomycete Compounds from the Biocontrol Agent Streptomyces sp. NEAU-S7GS2

Xie,

Du,

et al. 2024

J. Agric. Food Chem.

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Oomycetes are well-known phytopathogens that seriously threaten many important crops worldwide. In this study, the endophytic actinobacterium Streptomyces sp. NEAU-S7GS2 demonstrated significant antagonistic activity against Phytophthora and Pythium and showed a potent biocontrol effect on suppression of soybean phytophthora root rot and pepper phytophthora blight. Two compounds were subsequently isolated as the main active components by bioassay-guided fractionation and identified as lydicamycins A and B. These two compounds showed high antioomycete activity against Phytophthora and Pythium with EC 50 values of 0.73−2.67 μg/mL, which are equal to or lower than those of commercialized drug metalaxyl. In vivo bioassay using detached leaves demonstrated that lydicamycin A had a better control efficiency against soybean phytophthora root rot than metalaxyl. Taken together, these results suggest that the biocontrol agent Streptomyces sp. NEAU-S7GS2 and lydicamycins have the potential to be developed as promising pesticides to control diseases caused by oomycetes.

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“…KEGG highly enriched pathways include “valine, leucine, and isoleucine degradation” (map00280), “peroxisome” (map04146), “fatty acid degradation” (map00071), “propanoate metabolism” (map00640), “longevity regulating pathway-multiple species” (map04213), and “pyruvate metabolism” (map00620). Relevant studies have shown that these pathways were associated with energy and material metabolism, redox processes, biofilms, and genetic information . Through cluster analysis, it was found that the related DEGs of P.…”

Section: Ph- and Pectinase-responsive Release Behaviors Of Hon From Scrpmentioning

confidence: 99%

“…Relevant studies have shown that these pathways were associated with energy and material metabolism, redox processes, biofilms, and genetic information. 44 Through cluster analysis, it was found that the related DEGs of P. nicotianae treated with SCRP had significant changes in oxidative stress, membrane stability, and material energy metabolism (Figure 4C).…”

Section: Ph-and Pectinase-responsive Release Behaviors Of Hon From Scrpmentioning

confidence: 99%

A Smart Pesticide-Controlled Release Platform with Dual Stimuli-Responsive Functions for Enhanced Treatment of Plant Black Shank

Xie,

Zhang,

et al. 2024

ACS Appl. Mater. Interfaces

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Realizing controllable input of botanical pesticides is conducive to improving pesticide utilization, reducing pesticide residues, and avoiding environmental pollution but is extremely challenging. Herein, we constructed a smart pesticide-controlled release platform (namely, SCRP) for enhanced treatment of tobacco black shank based on encapsulating honokiol (HON) with mesoporous hollow structured silica nanospheres covered with pectin and chitosan oligosaccharide (COS). The SCRP has a loading capacity of 12.64% for HON and could effectively protect HON from photolysis. Owing to the pH-and pectinase-sensitive property of the pectin, the SCRP could smartly release HON in response to a low pH or a rich pectinase environment in the black shank-affected area. Consequently, the SCRP effectively inhibits the infection of P. nicotianae on tobacco with a controlled rate for tobacco black shank of up to 87.50%, which is mainly due to the SCRP's capability in accumulating ROS, changing cell membrane permeability, and affecting energy metabolism. In addition, SCRP is biocompatible, and the COS layer enables SCRP to show a significant growth-promoting effect on tobacco. These results indicate that the development of a stimuli-responsive controlled pesticide release system for plant disease control is of great potential and value for practical agriculture production.

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2 E ,4 E -Decadienoic Acid, a Novel Anti-Oomycete Agent from Coculture of Bacillus subtilis and Trichoderma asperellum

Abstract: P . nicotianae is an oomycete pathogen that is destructive to crops. Although some oomycete inhibitors have been used during crop production, most are harmful to the ecology and lead to pathogen resistance.

Cited by 5 publications

References 72 publications

Metabolomic profiles of the liquid state fermentation in co-culture of Eurotium amstelodami and Bacillus licheniformis

Metabolomic profiles of the liquid state fermentation in co-culture of Eurotium amstelodami and Bacillus licheniformis

Identification of Lydicamycins as Main Antioomycete Compounds from the Biocontrol Agent Streptomyces sp. NEAU-S7GS2

A Smart Pesticide-Controlled Release Platform with Dual Stimuli-Responsive Functions for Enhanced Treatment of Plant Black Shank

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