[2] Families of serine peptidases

Rawlings, Neil D.; Barrett, Alan J.

doi:10.1016/0076-6879(94)44004-2

Cited by 558 publications

(448 citation statements)

References 98 publications

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“…Although no significant protease sequence identities were found outside the catalytic sites, the MyxSP-1 protein sequence did contain the conserved catalytic triad and surrounding amino acid residues that are characteristic for serine proteases (present Fig. 2, and Rawlings & Barrett 1994, Barrett & Rawlings 1995. In addition, Rawlings & Barrett (1994) reported that a key aspect of the evolutionary division of serine proteases is based on the linear sequence arrangement of the catalytic residues.…”

Section: Discussionmentioning

confidence: 86%

“…2, and Rawlings & Barrett 1994, Barrett & Rawlings 1995. In addition, Rawlings & Barrett (1994) reported that a key aspect of the evolutionary division of serine proteases is based on the linear sequence arrangement of the catalytic residues. The linear sequence data for the catalytic triad of MyxSP-1 (His-54, Asp-248, Ser-310) indicates an evolutionary affinity with chymotrypsin-like serine proteases.…”

Section: Discussionmentioning

confidence: 99%

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Identification of a serine protease gene expressed by Myxobolus cerebralis during development in rainbow trout Oncorhynchus mykiss

et al. 2004

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Serine proteases have been recognized as key factors in parasite physiology and disease development. We have identified a serine protease gene from Myxobolus cerebralis, MyxSP-1, the myxozoan parasite causing whirling disease in salmonid fishes. The amino acid sequence, as deduced from the cDNA sequence, included a catalytic residue arrangement similar to that of the chymotrypsin family of serine proteases. A real-time TaqMan polymerase chain reaction (PCR) analysis revealed differences in the transcription levels for the chymotrypsin-like protease as found in early, intermediate, and late developmental stages of the parasite in experimentally-infected rainbow trout Oncorhynchus mykiss. MyxSP-1 transcription differed between individual tissues at each sampling point and in the same tissues over time (p < 0.0001). A nonradioactive mRNA in situ hybridization (ISH) protocol was developed to detect MyxSP-1 transcripts. Using a mixture of 3 digoxigenin-labeled antisense mRNA probes, MyxSP-1 transcription was observed in developmental stages of the parasite during the acute and chronic phases of the disease over a 240 d time period in infected rainbow trout tissues. MyxSP-1 transcription observed by ISH in cartilage and as associated with cartilage destruction was consistent with our real-time TaqMan PCR findings that demonstrated high levels of MyxSP-1 transcription during lesion development. Identifying genes encoding these enzymes and characterization of their functions can lead to the development of new chemotherapeutic protocols and vaccine approaches to control parasitic diseases.

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Section: Discussionmentioning

confidence: 86%

Section: Discussionmentioning

confidence: 99%

Identification of a serine protease gene expressed by Myxobolus cerebralis during development in rainbow trout Oncorhynchus mykiss

et al. 2004

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“…In the present study, three conserved active sites associated with the enzyme activity, Cys141, His280 and Asn300, were found in CpCL. The second amino acids of CpCL mature peptide was Pro, which might be helpful to keep the N-end of the protein being unhydrolyzed [27]. In addition, six potential substrate binding sites, Leu185, Met186, Ala251, Asn278, Gly281 and Ala 327 were observed in CpCL, which exhibited a marked preference for bulky hydrophobic or aromatic residues in the sidechain position of the substrate [28].…”

Section: Discussionmentioning

confidence: 99%

Molecular cloning and characterization of cathepsin L from freshwater mussel, Cristaria plicata

et al. 2014

Fish & Shellfish Immunology

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“…1d) similar to other proteases, including that encoded by the homologous gene TMPRSS2, which also maps on 21q, centromeric to the DFNB10 critical region 10 . The serine protease domain (residues 217-444) is compatible with the S1 family of the PA clan of serine-type peptidases (serine or cysteine nucleophile; catalytic residues in the order His, Asp, Ser (or Cys) in sequence; all endopeptidases) for which the prototype is chymotrypsin 11 , and shows between 45 and 38% identity with other transmembrane serine proteases (Fig. 1f).…”

Section: Division Of Medical Genetics University Of Geneva Medicalmentioning

confidence: 98%

Insertion of β-satellite repeats identifies a transmembrane protease causing both congenital and childhood onset autosomal recessive deafness

et al. 2001

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Approximately 50% of childhood deafness is caused by mutations in specific genes. Autosomal recessive loci account for approximately 80% of nonsyndromic genetic deafness 1 . Here we report the identification of a new transmembrane serine protease (TMPRSS3; also known as ECHOS1) expressed in many tissues, including fetal cochlea, which is mutated in the families used to describe both the DFNB10 and DFNB8 loci. An 8-bp deletion and insertion of 18 monomeric (∼68-bp) β-satellite repeat units, normally present in tandem arrays of up to several hundred kilobases on the short arms of acrocentric chromosomes, causes congenital deafness (DFNB10). A mutation in a spliceacceptor site, resulting in a 4-bp insertion in the mRNA and a frameshift, was detected in childhood onset deafness (DFNB8). This is the first description of β-satellite insertion into an active gene resulting in a pathogenic state, and the first description of a protease involved in hearing loss.

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[2] Families of serine peptidases

Cited by 558 publications

References 98 publications

Identification of a serine protease gene expressed by Myxobolus cerebralis during development in rainbow trout Oncorhynchus mykiss

Identification of a serine protease gene expressed by Myxobolus cerebralis during development in rainbow trout Oncorhynchus mykiss

Molecular cloning and characterization of cathepsin L from freshwater mussel, Cristaria plicata

Insertion of β-satellite repeats identifies a transmembrane protease causing both congenital and childhood onset autosomal recessive deafness

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