2  Evaluation of the microbiome effects of easy and hard keeper horses with the application of the Equine Keeper Status Scale

Johnson, A.; Biddle, Amy S.

doi:10.1016/j.jevs.2021.103465

Cited by 1 publication

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“…The Tenericutes phylum has been previously reported as a minoritarian phylum of the fecal microbiome of healthy horses [5,15]. The Euryarchaeota phylum of the kingdom archaea (not bacteria) also represents a minority, and it has been seen to be significantly more present in "hard keeper" horses that struggle to put on weight than in horses in other Equine Keeper Status Scale (EKSS) groups [35]. The proportion of target sequences within a community affects their detection through PCR-based methods.…”

Section: Discussionmentioning

confidence: 99%

Improved Equine Fecal Microbiome Characterization Using Target Enrichment by Hybridization Capture

Álvarez Narváez,

Beaudry,

Norris

et al. 2024

Animals

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GITDs are among the most common causes of death in adult and young horses in the United States (US). Previous studies have indicated a connection between GITDs and the equine gut microbiome. However, the low taxonomic resolution of the current microbiome sequencing methods has hampered the identification of specific bacterial changes associated with GITDs in horses. Here, we have compared TEHC, a new approach for 16S rRNA gene selection and sequencing, with conventional 16S rRNA gene amplicon sequencing for the characterization of the equine fecal microbiome. Both sequencing approaches were used to determine the fecal microbiome of four adult horses and one commercial mock microbiome. Our results show that TEHC yielded significantly more operational taxonomic units (OTUs) than conventional 16S amplicon sequencing when the same number of reads were used in the analysis. This translated into a deeper and more accurate characterization of the fecal microbiome when the samples were sequenced with TEHC according to the relative abundance analysis. Alpha and beta diversity metrics corroborated these findings and demonstrated that the microbiome of the fecal samples was significantly richer when sequenced with TEHC compared to 16S amplicon sequencing. Altogether, our study suggests that the TEHC strategy provides a more extensive characterization of the fecal microbiome of horses than the current alternative based on the PCR amplification of a portion of the 16S rRNA gene.

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Section: Discussionmentioning

confidence: 99%

Improved Equine Fecal Microbiome Characterization Using Target Enrichment by Hybridization Capture

Álvarez Narváez,

Beaudry,

Norris

et al. 2024

Animals

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2  Evaluation of the microbiome effects of easy and hard keeper horses with the application of the Equine Keeper Status Scale

Cited by 1 publication

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Improved Equine Fecal Microbiome Characterization Using Target Enrichment by Hybridization Capture

Improved Equine Fecal Microbiome Characterization Using Target Enrichment by Hybridization Capture

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2 Evaluation of the microbiome effects of easy and hard keeper horses with the application of the Equine Keeper Status Scale

Cited by 1 publication

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Improved Equine Fecal Microbiome Characterization Using Target Enrichment by Hybridization Capture

Improved Equine Fecal Microbiome Characterization Using Target Enrichment by Hybridization Capture

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2  Evaluation of the microbiome effects of easy and hard keeper horses with the application of the Equine Keeper Status Scale