2‑Deoxy‑D‑glucose enhances the anti‑cancer effects of idarubicin on idarubicin‑resistant P388 leukemia cells

Matsuo, Taisuke; Konya, Yumiko; Hirayama, Eri; Sadzuka, Yasuyuki

doi:10.3892/ol.2020.11616

Cited by 2 publications

(2 citation statements)

References 24 publications

(23 reference statements)

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“…To image the intestine, we used red fluorescent glucose (Glucose Uptake Assay Kit-Red; excitation wavelength, 560 nm; fluorescence wavelength, 572 nm; Dojindo Laboratories, Japan) to label OP50-1 cells in accordance with the manufacturer’s protocol 47 , 48 . Bacteria were washed three times with 2 mL of phosphate-buffered saline (pH 7.4) for 10 min at 2000 g in a centrifuge (EX-126, Tomy Seiko Co., Ltd., Japan).…”

Section: Methodsmentioning

confidence: 99%

Reciprocating intestinal flows enhance glucose uptake in C. elegans

Suzuki

Kikuchi

Numayama-Tsuruta

et al. 2022

Sci Rep

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Despite its physiological and pathological importance, the mechanical relationship between glucose uptake in the intestine and intestinal flows is unclear. In the intestine of the nematode Caenorhabditis elegans, the defecation motor program (DMP) causes reciprocating intestinal flows. Although the DMP is frequently activated in the intestines, its physiological function is unknown. We evaluated the mechanical signature of enhanced glucose uptake by the DMP in worms. Glucose uptake tended to increase with increasing flow velocity during the DMP because of mechanical mixing and transport. However, the increase in input energy required for the DMP was low compared with the calorie intake. The findings suggest that animals with gastrointestinal motility exploit the reciprocating intestinal flows caused by peristalsis to promote nutrient absorption by intestinal cells.

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Section: Methodsmentioning

confidence: 99%

Reciprocating intestinal flows enhance glucose uptake in C. elegans

Suzuki

Kikuchi

Numayama-Tsuruta

et al. 2022

Sci Rep

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“…Glucose consumption and lactate production were measured using the Glucose Assay Kit-WST and Lactate Assay Kit-WST (Dojindo Laboratories, Kumamoto, Japan) as described previously (Matsuo et al, 2020 ).…”

Section: Methodsmentioning

confidence: 99%

MicroRNA-505, Suppressed by Oncogenic Long Non-coding RNA LINC01448, Acts as a Novel Suppressor of Glycolysis and Tumor Progression Through Inhibiting HK2 Expression in Pancreatic Cancer

Zhang

et al. 2021

Front. Cell Dev. Biol.

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Background: MicroRNAs (miRNAs) and long non-coding RNAs (lncRNAs) play vital regulatory roles in pancreatic cancer (PC) initiation and progression. We aimed to explore the biological functions and underlying mechanisms of miR-505-3p (miR-505) in PC.Methods: We first screened miRNA expression profiles using microarray in PC tissues and normal tissues, and then studied the function and underlying mechanism of miR-505. Moreover, we evaluated the regulatory effect of lncRNA LINC01448 on miR-505.Results: We demonstrated miR-505 that was significantly downregulated in PC tissues. We further revealed that miR-505 significantly inhibited cell proliferation, invasion, sphere formation, glucose consumption, and lactate production by targeting HK2. In addition, overexpression of miR-505 led to tumor growth inhibition in vivo, demonstrating that it acts as a tumor suppressor in PC. LINC01448 was identified as an oncogenic lncRNA that could reduce miR-505 expression. Subsequent studies confirmed that LINC01448 enhanced cell proliferation, invasion, sphere formation, glucose consumption, and lactate production by regulating the miR-505/HK2 pathway.Conclusions: These findings demonstrated that miR-505, suppressed by LINC01448, could function as a key tumor suppressor by targeting HK2 in PC, elucidating an important role of the LINC01448/miR-505/HK2 pathway in regulating PC glycolysis and progression.

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2‑Deoxy‑D‑glucose enhances the anti‑cancer effects of idarubicin on idarubicin‑resistant P388 leukemia cells

Cited by 2 publications

References 24 publications

Reciprocating intestinal flows enhance glucose uptake in C. elegans

Reciprocating intestinal flows enhance glucose uptake in C. elegans

MicroRNA-505, Suppressed by Oncogenic Long Non-coding RNA LINC01448, Acts as a Novel Suppressor of Glycolysis and Tumor Progression Through Inhibiting HK2 Expression in Pancreatic Cancer

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