2-Aminophenol and 4-Aminophenol Toxicity in Renal Slices From Sprague-Dawley and Fischer 344 Rats

Abstract

This study examined differences in toxicity between 2- and 4-aminophenol using a renal cortical slice model. Renal cortical slice toxicity for 2- and 4-aminophenol was also monitored in tissue from Sprague-Dawley and Fischer 344 (F344) rats in order to determine potential strain differences for aminophenol toxicity. Renal cortical slices from Sprague-Dawley and F344 rats (age 50-65 d) were isolated and incubated for 15-120 min with 0-1 mM 2- or 4-aminophenol at 37 degrees C under an oxygen atmosphere. Elevations in lactate dehydrogenase (LDH) leakage from renal cortical slices occurred at lower concentrations of 4-aminophenol than of 2-aminophenol from both strains of rats. Total glutathione levels were more markedly decreased by 4-aminophenol than by 2-aminophenol in renal slices from both strains. LDH release was elevated by 1 mM 2-aminophenol in renal slices from F344 rats, but values were comparable between control and treated in the renal slices from Sprague-Dawley rats. 4-Aminophenol was slightly more toxic to renal slices from F344 than from Sprague-Dawley rats. LDH release was increased, relative to controls, by 0.1 mM in the F344 rats group compared to 0.25 mM in the Sprague-Dawley group. Strain differences were not apparent when comparisons were made of total glutathione levels or rate-limiting substrates of gluconeogenesis. These results indicated that strain differences in toxicity were detected between Sprague-Dawley and F344 rat strains. Based on LDH release, renal cortical slices obtained from age-matched F344 rats were slightly more susceptible than Sprague-Dawley rats to toxicity by 2- and 4-aminophenol.

Assessment of Mastitis Patterns in Serbian Dairy Cows: Blood Serum Metabolic Profile and Milk Composition Parameters

“…Moreover, these authors recorded higher blood serum concentrations of LDH and ALP in cows with subclinical mastitis compared to healthy ones. Most of the cells contain LDH, and when these cells are lethally injured, the loss of membrane integrity can be assessed by monitoring the activity of LDH [54]. Our results indicate that the concentration of LDH in blood serum shows a positive correlation with the concentration of LDH in milk, as well as the total protein and SCC in milk, while a negative correlation was found with the lactose in milk.…”

4-Amino-2-chlorophenol: Comparative in vitro nephrotoxicity and mechanisms of bioactivation

“…[8]). In general, 4-aminophenols appear to be more potent nephrotoxicants than 2-aminophenols [8,14,26]. However, comparative studies examining the effect of chloro groups on 4-AP nephrotoxicity in vitro are limited to a comparison of 4-AP and 4-amino-3-chlorophenol (4-A3CP) in rat renal cortical slices [8,27].…”