2.45 GHz radiofrequency fields alter gene expression in cultured human cells

Lee, Sanggyu; Johnson, Debra; Dunbar, Kerry B.; Dong, Hui; Ge, Xijin; Kim, Yeong C.; Wing, Claudia; Jayathilaka, Nimanthi; Emmanuel, Nimmi; Zhou, Chenn Q.; Gerber, H.L.; Tseng, Charles C.; Wang, San Ming

doi:10.1016/j.febslet.2005.07.063

Cited by 83 publications

(53 citation statements)

References 37 publications

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“…In MW therapy, conversive heat using 0.3-3.0 GHz of electromagnetic wave (2.45 GHz is typically used for clinical applications) is applied to a target area using a magnetron tube via an applicator, and is believed to be effective in increasing deep temperature. Although MW has nonthermal effects on cellular biological activity (e.g., apoptotic-related signaling and cell cycle), 17 it has been shown that MW was useful to deliver heat stimulation to deep tissues in the articular joint. 18 MW therapy is a well-recognized treatment modality for joint disease and is widely used in clinical settings.…”

Section: Discussionmentioning

confidence: 99%

Effects of heat stimulation via microwave applicator on cartilage matrix gene and HSP70 expression in the rabbit knee joint

Tonomura

Takahashi

Mazda

et al. 2007

Journal Orthopaedic Research

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The objective of this study was to investigate the effects of heat stimulation on the expression of extracellular matrix genes and heat-shock protein 70 (HSP70) in rabbit articular cartilage in vivo. Heat stimulation was applied to the knee joints of Japanese white rabbits for 20 min using a microwave (MW) applicator (2.45-GHz, 0-80 W). After 8-72 h, the articular cartilage was removed from the knee joints and proteins and total RNA were extracted. As controls, knee joints without heat stimulation were analyzed. The expression of HSP70 was confirmed by real-time PCR and Western blotting. The expression of proteoglycan core protein (PG) and type II collagen (Col II) was quantified using real-time PCR to assess cartilage matrix metabolism. Compared to controls, HSP70 expression was higher with more than 40 W of heat stimulation. The expression of PG and Col II mRNA was higher, with more than 20 W of heat stimulation and peaked with 40 W. When quercetin was used to inhibit the induction of HSP70 expression, PG mRNA expression did not increase. External MW application stimulated HSP70 expression in the articular cartilage in vivo. The expression of extracellular matrix genes was increased by appropriate heat stimulation. ß

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Section: Discussionmentioning

confidence: 99%

Effects of heat stimulation via microwave applicator on cartilage matrix gene and HSP70 expression in the rabbit knee joint

Tonomura

Takahashi

Mazda

et al. 2007

Journal Orthopaedic Research

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“…Therefore, the hazardous effects of EMFs on living systems are increasing (Berg, 1995;Goodman et al, 1995). It has been documented that EMFs affect the cell protein (Kwee et al, 2001), change the cell membrane characteristics (Goltsov, 1999), and alter the enzyme activity (Paulraj and Behari, 2002;Barteri et al, 2004) and gene expression (Lee et al, 2005) in animals. Additionally, radiofrequency EMFs induce lipid peroxidation and heat shock proteins, and elicit antioxidant response in human cells (Kwee et al, 2001;Moustafa et al, 2001;Leszczynski et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Cell Phone Radiations Affect Early Growth of Vigna radiata (Mung Bean) through Biochemical Alterations

Sharma

Singh

Batish

et al. 2010

Zeitschrift Für Naturforschung C

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The indiscriminate use of wireless technologies, particularly of cell phones, has increased the health risks among living organisms including plants. We investigated the impact of cell phone electromagentic fi eld (EMF) radiations (power density, 8.55 μW cm -2 ) on germination, early growth, proteins and carbohydrate contents, and activities of some enzymes in Vigna radiata. Cell phone EMF radiations signifi cantly reduced the seedling length and dry weight of V. radiata after exposure for 0.5, 1, 2, and 4 h. Furthermore, the contents of proteins and carbohydrates were reduced in EMF-exposed plants. However, the activities of proteases, α-amylases, β-amylases, polyphenol oxidases, and peroxidases were enhanced in EMF-exposed radicles indicating their role in providing protection against EMF-induced stress. The study concludes that cell phone EMFs impair early growth of V. radiata seedlings by inducing biochemical changes.

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“…Exposure of 2.45 GHz high frequency electromagnetic field to glioma cells at SAR levels above 20 W/kg led to an increased level of HSP70, even when the effect of raised temperature is taken into account (Tian et al, 2002 radiation at 10 W/kg of SAR for 2∼6 h affected expression of 221 genes including apoptosis-related genes and the cell cycle genes in human HL60 cells without significant increase of HSP expression (Lee et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Classification of Biological Effect of 1,763 MHz Radiofrequency Radiation Based on Gene Expression Profiles

Im¹,

Kim²,

Park³

et al. 2010

Genomics & Informatics

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Radiofrequency (RF) radiation might induce the transcription of a certain set of genes as other physical stresses like ionizing radiation and UV. To observe transcriptional changes upon RF radiation, we exposed WI-38, human lung fibroblast cell to 1763 MHz of mobile phone RF radiation at 60 W/kg of specific absorption rate (SAR) for 24h with or without heat control. There were no significant changes in cell numbers and morphology after exposure to RF radiation. Using quantitative RT-PCR, we checked the expression of three heat shock protein (HSP) (HSPA1A, HSPA6 and HSP105) and seven stress-related genes (TNFRSF11B, FGF2, TGFB2, ITGA2, BRIP1, EXO1, and MCM10) in RF only and RF/HS groups of RF-exposed cells. The expressions of three heat shock proteins and seven stress-related genes were selectively changed only in RF/HS groups. Based on the expression of ten genes, we could classify thermal and non-thermal effect of RF-exposure, which genes can be used as biomarkers for RF radiation exposure.

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2.45 GHz radiofrequency fields alter gene expression in cultured human cells

Cited by 83 publications

References 37 publications

Effects of heat stimulation via microwave applicator on cartilage matrix gene and HSP70 expression in the rabbit knee joint

Effects of heat stimulation via microwave applicator on cartilage matrix gene and HSP70 expression in the rabbit knee joint

Cell Phone Radiations Affect Early Growth of Vigna radiata (Mung Bean) through Biochemical Alterations

Classification of Biological Effect of 1,763 MHz Radiofrequency Radiation Based on Gene Expression Profiles

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