2′,4′-Dihydroxy-6′‑methoxy-3′,5′-dimethylchalcone and its amino acid-conjugated derivatives induce G0/G1 cell cycle arrest and apoptosis via BAX/BCL2 ratio upregulation and in silico insight in SiHa cell lines

Utama, Kraikrit; Khamto, Nopawit; Meepowpan, Puttinan; Aobchey, Paitoon; Kantapan, Jiraporn; Meerak, Jomkhwan; Roytrakul, Sittiruk; Sangthong, Padchanee

doi:10.1016/j.ejps.2023.106390

Cited by 5 publications

(2 citation statements)

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“…42,43 In this study, we investigated the impact of dietary inclusion of CP on the degree of cell proliferation and apoptosis in the intestinal epithelial cells of largemouth bass by examining the expression levels of genes encoding Bcl-2 family proteins (the genes encoding the pro-apoptotic proteins Bax and Bad were bax and bad, respectively; and the genes encoding the anti-apoptotic proteins Bcl-xL and Bcl-2 were Bcl-xl and bcl-2, respectively), the expression level of the gene encoding the anti-apoptotic protein BAG (the bag gene), the expression level of the gene encoding caspase-3 (the casp3 gene), and changes in the bax/bcl2 ratio. 44,45 When the level of CP in feed ranged from 3% to 12%, the expression of the Bcl-xl and bag genes was significantly upregulated. The dietary inclusion of CP significantly decreased the expression levels of pro-apoptotic genes (the bax, bad, and casp3 genes) and the bax/bcl2 ratio, except when the level of CP in feed was 3%.…”

Section: Discussionmentioning

confidence: 99%

“…It is tightly regulated by multiple genes that are highly conserved across species, especially genes encoding proteins of the Bcl‐2 family or the caspase family 42,43 . In this study, we investigated the impact of dietary inclusion of CP on the degree of cell proliferation and apoptosis in the intestinal epithelial cells of largemouth bass by examining the expression levels of genes encoding Bcl‐2 family proteins (the genes encoding the pro‐apoptotic proteins Bax and Bad were bax and bad , respectively; and the genes encoding the anti‐apoptotic proteins Bcl‐xL and Bcl‐2 were Bcl‐xl and bcl‐2 , respectively), the expression level of the gene encoding the anti‐apoptotic protein BAG (the bag gene), the expression level of the gene encoding caspase‐3 (the casp3 gene), and changes in the bax / bcl2 ratio 44,45 . When the level of CP in feed ranged from 3% to 12%, the expression of the Bcl‐xl and bag genes was significantly upregulated.…”

Section: Discussionmentioning

confidence: 99%

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Effects of dietary citrus pulp level on the growth and intestinal health of largemouth bass (Micropterus salmoides)

Long,

Luo,

et al. 2023

J Sci Food Agric

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BACKGROUNDCitrus pulp (CP) is rich in pectin, and studies have shown that pectin possesses antioxidant, anti‐inflammatory, and gut microbiota‐regulating properties. However, the application of CP in aquafeed is limited. In this study, the effect of dietary inclusion of CP on the intestinal health of largemouth bass (Micropterus salmoides) was investigated. Juveniles of similar size (6.95 ± 0.07 g) were fed isonitrogenous and isoenergetic diets containing different levels of CP (0%, 3%, 6%, 9%, 12%, or 15%) for 58 days.RESULTSAs the level of CP in the feed for largemouth bass increased, the fish's growth performance and intestinal health initially improved and then declined. Adding low doses of CP (≤9%) to the feed had no significant impact on the growth performance of large‐mouth black bass, whereas high doses of CP (>9%) significantly reduced their growth performance. Adding 6%, 9%, or 12% of CP to that feed enhanced the expression of genes related to tight junctions, anti‐inflammatory activity, anti‐apoptotic activity, and antioxidant activity in the intestines of largemouth bass. It reduced intestinal inflammation and improved intestinal nutrient absorption, intestinal mucosal barrier function, and intestinal antioxidant capacity. Moreover, it improved the α‐diversity, structure, and function of the intestinal flora. The addition of 6% CP had the most beneficial effect on the intestinal health of largemouth bass. On the other hand, the addition of 15% CP had adverse effects on the intestinal antioxidant capacity and intestinal mucosal barrier function of largemouth bass.CONCLUSIONAdding 6–9% CP to the feed for largemouth bass can improve their intestinal health without having a significant impact on their growth performance. CP could serve as a novel prebiotic and immunostimulant ingredient in aquafeed. © 2023 Society of Chemical Industry.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%