2,4-Diacetylphloroglucinol: A Novel Biotech Bioactive Compound for Agriculture

Pseudomonas spp. be néficas e n la a gricultura

“…This genus has an exceptional capacity to produce a wide variety of antibiotics, some of them of the polyketide type such as 2,4-diacetylphloroglucinol (DAPG), mupirocin and pyoluteorin (Bender et al, 1999;Kankariya et al, 2019), as well as pyrrolnitrin, compounds derived from phenazine, and cyclic lipopeptides such as orphamide A, Pseudofactin and viscosin (Figure 2) (Quan et al, 2010;Jang et al, 2013;Ma et al, 2016;Malviya et al, 2020), the production of these compounds is modulated by exogenous, biotic or abiotic factors, such as the addition of fertilizers, the carbon source and the minerals that influence their synthesis. For example, the addition of glucose to the growth medium of Pseudomonas significantly increases the production of DAPG, while the supplementation of the culture medium with phosphate represses its synthesis (Kumar et al, 2017;Kankariya et al, 2019).…”

“…This genus has an exceptional capacity to produce a wide variety of antibiotics, some of them of the polyketide type such as 2,4-diacetylphloroglucinol (DAPG), mupirocin and pyoluteorin (Bender et al, 1999;Kankariya et al, 2019), as well as pyrrolnitrin, compounds derived from phenazine, and cyclic lipopeptides such as orphamide A, Pseudofactin and viscosin (Figure 2) (Quan et al, 2010;Jang et al, 2013;Ma et al, 2016;Malviya et al, 2020), the production of these compounds is modulated by exogenous, biotic or abiotic factors, such as the addition of fertilizers, the carbon source and the minerals that influence their synthesis. For example, the addition of glucose to the growth medium of Pseudomonas significantly increases the production of DAPG, while the supplementation of the culture medium with phosphate represses its synthesis (Kumar et al, 2017;Kankariya et al, 2019). DAPG is a phenolic-type antibiotic, a broad spectrum of inhibition against bacteria, fungi, nematodes has been reported, it has been associated with suppressive soils and is a key component in the biocontrol of root and seedling diseases; such as black root rot in tobacco plants, control of phytopathogenic fungi such as Thielaviopsis basicola, Pythium ultimum, Gaeumannomyces graminis var tritici (Keel et al, 1990;Bender et al, 1999;Weller et al, 2007;Kankariya et al, 2019;Keswani et al, 2020); likewise, it has been observed that, at low concentrations, it has beneficial effects on plant growth.…”

“…Pseudomonas are aerobic, gram-negative, chemoheterotrophic, motile, bacillus-shaped bacteria, belonging to the class Gammaproteobacteria and the family Pseudomonadaceae that includes approximately 191 different species (Kumar et al, 2017), due to their metabolic versatility and genetic plasticity, they are ubiquitous in different terrestrial and aquatic ecosystems. Currently, the genus has become relevant in the agricultural field due to the influence exerted by these bacteria on growth promotion, induced systemic resistance (ISR) in plants and the control of phytopathogens, through different mechanisms of action, among which are the production of various antimicrobial compounds such as 2,4-diacetylphloroglucinol (Kankariya et al, 2019) and cellulolytic enzymes (Kumar et al, 2017), among other metabolites, they produce phytohormones; they also improve the acquisition of nutrients through the production of siderophores (Wendenbaum et al, 1983).…”

Inhibitory efficacy of 2, 4-diacetylphloroglucinol against SARS-COV-2 proteins: in silico study

“…making it amenable to industrial fermentative production. DAPG has been reported for its antagonistic activity against bacteria, fungi, helminths, protozoa, cancerous cells, and mycobacteria (Kankariya et al 2019 ). Notably, DAPG has been reported to display strong activity against enveloped RNA and DNA viruses, such as (i) vesicular stomatitis virus (VSV), an enveloped RNA virus, and (ii) herpes simplex virus (HSV-I), an enveloped DNA virus, but unable to affect non-enveloped RNA virus (polio-I) (Tada et al 1990 ).…”

2,4-Diacetylphloroglucinol Against Candida albicans: Biofilm Formation, Aspartyl Protease Production and Ultrastructure Changes

“…2,4-DAPG is not a potent antibiotic comparable to amphotericin B, for example. However, it is capable of exhibiting activity over a wide range of suppressed organisms (Kankariya et al 2019; Almario et al 2017). The prevalence of genes involved in the production of 2,4-DAPG and other phloroglucinol derivatives is very high among various species of Pseudomonas genus (Troppens et al 2013).…”