2′,3′-Cyclic Mononucleotide Metabolism and Possible Roles in Bacterial Physiology

Fontaine, Benjamin M.; Duggal, Yashasvika; Weinert, Emily E.

doi:10.1007/978-3-030-33308-9_36

Cited by 1 publication

(1 citation statement)

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“…Therefore, at least in the latter case, 2′,3′-cNMP formed by RNase I would be hydrolyzed by periplasmic CpdB. Recently, 2′,3′-cNMP have been proposed as a novel class of bacterial signals [ 50 , 51 , 52 , 53 ]. In E. coli , they have clear physiological effects on gene expression, flagellar motility, biofilm formation and acid tolerance.…”

Section: Resultsmentioning

confidence: 99%

Genomic Distribution of Pro-Virulent cpdB-like Genes in Eubacteria and Comparison of the Enzyme Specificity of CpdB-like Proteins from Salmonella enterica, Escherichia coli and Streptococcus suis

Ribeiro

Canales

Costas

et al. 2023

IJMS

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The cpdB gene is pro-virulent in avian pathogenic Escherichia coli and in Salmonella enterica, where it encodes a periplasmic protein named CpdB. It is structurally related to cell wall-anchored proteins, CdnP and SntA, encoded by the also pro-virulent cdnP and sntA genes of Streptococcus agalactiae and Streptococcus suis, respectively. CdnP and SntA effects are due to extrabacterial hydrolysis of cyclic-di-AMP, and to complement action interference. The mechanism of CpdB pro-virulence is unknown, although the protein from non-pathogenic E. coli hydrolyzes cyclic dinucleotides. Considering that the pro-virulence of streptococcal CpdB-like proteins is mediated by c-di-AMP hydrolysis, S. enterica CpdB activity was tested as a phosphohydrolase of 3′-nucleotides, 2′,3′-cyclic mononucleotides, linear and cyclic dinucleotides, and cyclic tetra- and hexanucleotides. The results help to understand cpdB pro-virulence in S. enterica and are compared with E. coli CpdB and S. suis SntA, including the activity of the latter on cyclic-tetra- and hexanucleotides reported here for the first time. On the other hand, since CpdB-like proteins are relevant to host-pathogen interactions, the presence of cpdB-like genes was probed in eubacterial taxa by TblastN analysis. The non-homogeneous genomic distribution revealed taxa with cpdB-like genes present or absent, identifying eubacteria and plasmids where they can be relevant.

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