2,3-Butanedione suppresses gray mold of postharvest fruit by activating the autophagy of Botrytis cinerea

Li, Guangjin; Chen, Yong; Zhang, Zhanquan; Li, Boqiang; Chen, Tong; Tian, Shiping

doi:10.1016/j.postharvbio.2022.112057

Cited by 13 publications

(4 citation statements)

References 44 publications

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“…Moreover, elevated levels of soluble carbohydrates, nucleic acids, and MDA were detected in B. cinerea subjected to 2-HE treatment. These outcomes suggest that 2-HE disrupts membrane permeability, triggers cytoplasmic leakage, and induces membrane lipid peroxidation, findings that align with those from studies on 3-Octanol (Zhang et al, 2023), 2-Phenylethanol (Zou et al, 2022), and 2,3-Butanedione (Li et al, 2022).…”

Section: Discussionsupporting

confidence: 76%

2-Heptanol inhibits Botrytis cinerea by accelerating amino acid metabolism and retarding membrane transport

Wu,

Wang,

Lin

et al. 2024

Front. Plant Sci.

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During the postharvest storage of tomatoes, they are susceptible to infection by Botrytis cinerea, leading to significant economic losses. This study evaluated the antifungal potential of 2-heptanol (2-HE), a volatile biogenic compound, against B. cinerea and explored the underlying antifungal mechanism. The results indicated that 2-HE effectively suppressed the growth of B. cinerea mycelia both in vivo and in vitro and stimulated the activities of antioxidative enzymes, including superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) in tomatoes. Furthermore, 2-HE reduced spore viability, compromised membrane integrity, and resulted in increased levels of extracellular nucleic acids, protein content, and membrane lipid peroxidation. Transcriptomic analysis revealed that 2-HE disrupted the membrane transport system and enhanced amino acid metabolism, which led to intracellular nutrient depletion and subsequent B. cinerea cell death. Additionally, the 2-HE treatment did not negatively impact the appearance or quality of the tomatoes. In conclusion, the findings of this study offer insights into the use of 2-HE as a biocontrol agent in food and agricultural applications.

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Section: Discussionsupporting

confidence: 76%

2-Heptanol inhibits Botrytis cinerea by accelerating amino acid metabolism and retarding membrane transport

Wu,

Wang,

Lin

et al. 2024

Front. Plant Sci.

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“…S7c). Meanwhile, the B. cinerea deletion mutant of BcPG1 ( Bcpg1 ) was generated (Liu et al ., 2023) and utilized in the infection assay on tomato WT and Slferl mutant. As shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

A receptor‐like kinase SlFERL mediates immune responses of tomato to Botrytis cinerea by recognizing BcPG1 and fine‐tuning MAPK signaling

Ji,

Liu,

Cui

et al. 2023

New Phytologist

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Summary FERONIA (FER) is a receptor‐like kinase showing versatile functions during plant growth, development, and responses to environmental stimuli. However, its functions during the interaction between fruit and necrotrophic fungal pathogens are still unclear. Combining reverse genetic approaches, physiological assays, co‐immunoprecipitation, protein phosphorylation identification, and site‐directed mutagenesis, we reported a tomato FER homolog SlFERL (Solanum lycopersicum FERONIA Like) involved in the immune responses to Botrytis cinerea invasion. The results indicated that SlFERL extracellular domain recognized and interacted with the secreted virulence protein BcPG1 from B. cinerea, further revealed that SlFERL triggered downstream signaling by phosphorylating SlMAP3K18 at Thr45, Ser49, Ser76, and Ser135. Moreover, we verified that SlMAP2K2 and SlMAP2K4 synergistically contributed to immune response of tomato to B. cinerea, in which SlFERL‐SlMAP3K18 module substantially modulated protein level and/or kinase activity of SlMAP2K2/SlMAP2K4. These findings reveal a new pattern‐triggered immune pathway, indicating that SlFERL participates in the immune responses to B. cinerea invasion via recognizing BcPG1 and fine‐tuning MAPK signaling.

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“…Bio-sourced antifungal substances have attracted broad attention recently for their prominent properties, such as being non-toxic, readily available, and environmentally friendly (Chen et al 2023a ). To date, a series of bio-sourced substances have been reported with remarkable antifungal activities and promising potential for controlling B. cinerea (Wang et al 2020 ; Cui et al 2021 ; Sun et al 2021 ; Li et al 2022a ; Zhang et al 2023 ). An in-depth understanding of the pathogenic mechanism of B. cinerea is conducive to discovering novel antifungal substances and promoting the practical application of biogenic substances.…”

Section: Introductionmentioning

confidence: 99%

Characterization of two SGNH family cell death-inducing proteins from the horticulturally important fungal pathogen Botrytis cinerea based on the optimized prokaryotic expression system

Zhang,

Chen

et al. 2024

Mol Horticulture

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Botrytis cinerea is one of the most destructive phytopathogenic fungi, causing significant losses to horticultural crops. As a necrotrophic fungus, B. cinerea obtains nutrients by killing host cells. Secreted cell death-inducing proteins (CDIPs) play a crucial role in necrotrophic infection; however, only a limited number have been reported. For high-throughput CDIP screening, we optimized the prokaryotic expression system and compared its efficiency with other commonly used protein expression systems. The optimized prokaryotic expression system showed superior effectiveness and efficiency and was selected for subsequent CDIP screening. The screening system verified fifty-five candidate proteins and identified two novel SGNH family CDIPs: BcRAE and BcFAT. BcRAE and BcFAT exhibited high expression levels throughout the infection process. Site-directed mutagenesis targeting conserved Ser residues abolished the cell death-inducing activity of both BcRAE and BcFAT. Moreover, the transient expression of BcRAE and BcFAT in plants enhanced plant resistance against B. cinerea without inducing cell death, independent of their enzymatic activities. Our results suggest a high-efficiency screening system for high-throughput CDIP screening and provide new targets for further study of B. cinerea-plant interactions.

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2,3-Butanedione suppresses gray mold of postharvest fruit by activating the autophagy of Botrytis cinerea

Cited by 13 publications

References 44 publications

2-Heptanol inhibits Botrytis cinerea by accelerating amino acid metabolism and retarding membrane transport

2-Heptanol inhibits Botrytis cinerea by accelerating amino acid metabolism and retarding membrane transport

A receptor‐like kinase SlFERL mediates immune responses of tomato to Botrytis cinerea by recognizing BcPG1 and fine‐tuning MAPK signaling

Characterization of two SGNH family cell death-inducing proteins from the horticulturally important fungal pathogen Botrytis cinerea based on the optimized prokaryotic expression system

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