2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD)-induced suppression of immunity in THP-1-derived macrophages and the possible mechanisms

Li, Xinyan; Li, Na; Han, Yingnan; Rao, Kaifeng; Ji, Xiaoya; Ma, Ming

doi:10.1016/j.envpol.2021.117302

Cited by 15 publications

(17 citation statements)

References 82 publications

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“…The cell adhesion assay was performed as previously described. 15 Briefly, after 24 h of exposure, THP-1 mφs were fixed with 4% paraformaldehyde and then permeabilized with 0.1% Triton X-100 in 1% BSA/ PBS. Immunofluorescence staining was performed using a fluorophore dye cocktail containing Hoechst 33342 (5 μg/mL) and phalloidin (150 nM) to label the morphology of the THP-1 mφs.…”

Section: Methodsmentioning

confidence: 99%

“…The cell adhesion assay was performed as previously described . Briefly, after 24 h of exposure, THP-1 mφs were fixed with 4% paraformaldehyde and then permeabilized with 0.1% Triton X-100 in 1% BSA/PBS.…”

Section: Methodsmentioning

confidence: 99%

“…13 On the basis of our previous study, we developed a series of in vitro HCS approaches to evaluate the immunotoxicity of chemicals. 14,15 Here, we tried to apply this detecting system on the environmental samples for the first time. Yangtze River, the longest river in China and the third longest river in the world, is the most important inland water transportation route and drinking water source.…”

Section: Introductionmentioning

confidence: 99%

“…The in vitro high-content screening (HCS) assays, with the advantages of detecting all chemicals related to multiple given endpoints, are gradually becoming the trend of directly assessing the biological effect potential of environmental samples, including surface water . On the basis of our previous study, we developed a series of in vitro HCS approaches to evaluate the immunotoxicity of chemicals. , Here, we tried to apply this detecting system on the environmental samples for the first time.…”

Section: Introductionmentioning

confidence: 99%

“…,15 the reported concentrations of PFRs and TCDD equivalency (TEQ) values in the Nanjing section of the Yangtze River, (PFRs: 8.99−118.38 ng/L; 51−53 TEQ: 10.7− 97.7 pg/L…”

mentioning

confidence: 99%

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In Vitro Multibiomarker Approaches for Assessing the Immunotoxicity of Certain Sections in Yangtze River

et al. 2022

ACS EST Water

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Due to multiple endpoints and complex mechanisms, immunotoxicity tests were scarcely applied to environmental matrices. In this study, using a high-content screening system, a series of high-throughput multibiomarker approaches based on the immune functions of THP-1 macrophages (cell adhesion, morphology, phagocytosis, and production of multiple cytokines/chemokines) were adopted to evaluate the immunotoxicity of surface water extracts from the Yangtze River (near the Nanjing Chemical Industrial Park). The results suggested that the cell morphology and adhesion of macrophages were sensitive and appropriate for evaluating the surface water quality since both were closely associated with inflammation initiation and altered significantly when treated with only 10× water extracts. Twenty-four hours of exposure with water organic extracts (10× and 50×) suppressed cell phagocytosis, which was similar to the results of surface water extracts from southern Korea. Additionally, 30 cytokines/chemokines (including inflammatory cytokines; mediators of macrophages, T cells, and other immune cells; regulating neurons, angiogenesis, and anti-infection processes) were significantly altered by 50× water extracts. The top 10 cytokines are recommended as potential candidates for indicating surface water immunotoxicity. This research is the first study to evaluate many immunotoxicity endpoints with surface water extracts and offers fresh insights into the in vitro immunotoxic evaluation methods.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…,15 the reported concentrations of PFRs and TCDD equivalency (TEQ) values in the Nanjing section of the Yangtze River, (PFRs: 8.99−118.38 ng/L; 51−53 TEQ: 10.7− 97.7 pg/L…”

mentioning

confidence: 99%

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