2,3,4,5-Tetrakis(3′,4′-dihydroxylphenyl)thiophene: A New Matrix for the Selective Analysis of Low Molecular Weight Amines and Direct Determination of Creatinine in Urine by MALDI-TOF MS

Chen, Suming; Chen, Li; Wang, Jianbo; Hou, Jian; He, Qing; Liu, Jianan; Wang, Jiyun; Xiong, Shaoxiang; Yang, Guoqiang; Nie, Zongxiu

doi:10.1021/ac3021278

Cited by 63 publications

(36 citation statements)

References 47 publications

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“…DHB matrix peaks are indicated in red and root nodule tissue covered with DHB is shown in blue. Novel matrices such as TiO 2 nanoparticles 21 , 1,5diaminonapthalene (DAN) 22 , 2,3,4,5-Tetrakis(3′,4′-dihydroxylphenyl)thiophene (DHPT) 23 , and 1,8-bis(dimethyl-amino) naphthalene (DMAN) 24,25 have been reported that reduce the interference of matrix ions in the low mass range, and also enhance the detection of certain classes of metabolites 5 . Matrix peaks can be distinguished from real metabolites using the MS images.…”

Section: Representative Resultsmentioning

confidence: 99%

MALDI-Mass Spectrometric Imaging for the Investigation of Metabolites in <em>Medicago truncatula</em> Root Nodules

Gemperline

2014

JoVE

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Most techniques used to study small molecules, such as pharmaceutical drugs or endogenous metabolites, employ tissue extracts which require the homogenization of the tissue of interest that could potentially cause changes in the metabolic pathways being studied(1). Mass spectrometric imaging (MSI) is a powerful analytical tool that can provide spatial information of analytes within intact slices of biological tissue samples(1-5). This technique has been used extensively to study various types of compounds including proteins, peptides, lipids, and small molecules such as endogenous metabolites. With matrix-assisted laser desorption/ionization (MALDI)-MSI, spatial distributions of multiple metabolites can be simultaneously detected. Herein, a method developed specifically for conducting untargeted metabolomics MSI experiments on legume roots and root nodules is presented which could reveal insights into the biological processes taking place. The method presented here shows a typical MSI workflow, from sample preparation to image acquisition, and focuses on the matrix application step, demonstrating several matrix application techniques that are useful for detecting small molecules. Once the MS images are generated, the analysis and identification of metabolites of interest is discussed and demonstrated. The standard workflow presented here can be easily modified for different tissue types, molecular species, and instrumentation.

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Section: Representative Resultsmentioning

confidence: 99%

MALDI-Mass Spectrometric Imaging for the Investigation of Metabolites in <em>Medicago truncatula</em> Root Nodules

Gemperline

2014

JoVE

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“…The simplest way to address the first problem is to use an alternative MALDI matrix [8][9][10], which will generate a different set of matrix cluster ions that will hopefully not mask the signal of interest. However, different MALDI matrices have different ionization/desorption characteristics and may not enable adequate ionization of the target analyte.…”

Section: Introductionmentioning

confidence: 99%

Pyrylium Salts as Reactive Matrices for MALDI-MS Imaging of Biologically Active Primary Amines

Shariatgorji

Nilsson

Källback

et al. 2015

J. Am. Soc. Mass Spectrom.

101

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Abstract. Many neuroactive substances, including endogenous biomolecules, environmental compounds, and pharmaceuticals possess primary amine functional groups. Among these are catecholamine neurotransmitters (e.g., dopamine), many substituted phenethylamines (e.g., amphetamine), as well as amino acids and neuropeptides. In most cases, mass spectrometric (ESI and MALDI) analyses of trace amounts of such compounds are challenging because of their poor ionization properties. We present a method for chemical derivatization of primary amines by reaction with pyrylium salts that facilitates their detection by MALDI-MS and enables the imaging of primary amines in brain tissue sections. A screen of pyrylium salts revealed that the 2,4-diphenyl-pyranylium ion efficiently derivatizes primary amines and can be used as a reactive MALDI-MS matrix that induces both derivatization and desorption. MALDI-MS imaging with such matrix was used to map the localization of dopamine and amphetamine in brain tissue sections and to quantitatively map the distribution of the neurotoxin β-N-methylamino-L-alanine.

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“…This showed PAI-LDI has the potential for analyzing medicine metabolism. The mass spectrum (multi-point average) of urine sample showing the entire mass range, including spiked 1-adamantylamine and urine native creatinine [35], is shown in Supplementary Figure S3. The result shows that the components of urine have little influence on analyte signal.…”

Section: Analysis Of Real Samplesmentioning

confidence: 99%

Pyroelectricity Assisted Infrared-Laser Desorption Ionization (PAI-LDI) for Atmospheric Pressure Mass Spectrometry

Wei

et al. 2015

J. Am. Soc. Mass Spectrom.

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A new atmospheric pressure ionization method termed pyroelectricity-assisted infrared laser desorption ionization (PAI-LDI) was developed in this study. The pyroelectric material served as both sample target plate and enhancing ionization substrate, and an IR laser with wavelength of 1064 nm was employed to realize direct desorption and ionization of the analytes. The mass spectra of various compounds obtained on pyroelectric material were compared with those of other substrates. For the five standard substances tested in this work, LiNbO3 substrate produced the highest ion yield and the signal intensity was about 10 times higher than that when copper was used as substrate. For 1-adamantylamine, as low as 20 pg (132.2 fmol) was successfully detected. The active ingredient in (Compound Paracetamol and 1-Adamantylamine Hydrochloride Capsules), 1-adamantylamine, can be sensitively detected at an amount as low as 150 pg, when the medicine stock solution was diluted with urine. Monosaccharide and oligosaccharides in Allium Cepa L. juice was also successfully identified with PAI-LDI. The method did not require matrix-assisted external high voltage or other extra facility-assisted set-ups for desorption/ionization. This study suggested exciting application prospect of pyroelectric materials in matrix- and electricity-free atmospheric pressure mass spectrometry research.

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2,3,4,5-Tetrakis(3′,4′-dihydroxylphenyl)thiophene: A New Matrix for the Selective Analysis of Low Molecular Weight Amines and Direct Determination of Creatinine in Urine by MALDI-TOF MS

Cited by 63 publications

References 47 publications

MALDI-Mass Spectrometric Imaging for the Investigation of Metabolites in <em>Medicago truncatula</em> Root Nodules

MALDI-Mass Spectrometric Imaging for the Investigation of Metabolites in <em>Medicago truncatula</em> Root Nodules

Pyrylium Salts as Reactive Matrices for MALDI-MS Imaging of Biologically Active Primary Amines

Pyroelectricity Assisted Infrared-Laser Desorption Ionization (PAI-LDI) for Atmospheric Pressure Mass Spectrometry

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