2,2‘-Diphenyl-1-picrylhydrazyl Radical-Scavenging Active Components from Adlay (Coix lachryma-jobi L. Var. ma-yuen Stapf) Hulls

Abstract

An activity-directed fractionation and purification process was used to identify the antioxidative components of adlay hulls. Hulls of adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) were extracted with methanol and then separated into water, 1-butanol, ethyl acetate, and hexane fractions. The 1-butanol-soluble fraction exhibited greater capacity to scavenge 2,2'-diphenyl-1-picrylhydrazyl (DPPH) radicals when compared with fractions soluble in water, ethyl acetate, and hexane phases. The 1-butanol fraction was then subjected to separation and purification using Diaion HP-20 chromatography, silica gel chromatography, and HPLC. Six compounds showing strong antioxidant activity were identified by spectroscopic methods ((1)H NMR, (13)C NMR, IR, and MS) and by comparison with authentic samples to be coniferyl alcohol (1), syringic acid (2), ferulic acid (3), syringaresinol (4), 4-ketopinoresinol (5), and a new lignan, mayuenolide (6).

Downregulation of progesterone biosynthesis in rat granulosa cells by adlay (Coix lachryma-jobi L. var. ma-yuen Stapf.) bran extracts

“…The preparation of adlay extracts was from the previously described method, with minor modifications. 7 Adlay brans were blended in powder form and screened through a 20-mesh sieve (aperture 0.94 mm). The powder of adlay bran (100 g) was extracted with 1 l of methanol stirred on a stirring plate at room temperature for 24 h. Contents were filtered through #1 filter paper (Whatman Inc., Hillsboro, OR, USA).…”

“…The solvent (water, butanol, ethyl acetate, n-hexane) was concentrated to dryness in vacuum condition to give dried powder and stored at À201C until use. ABM-Bu was further fractionated using Diaion HP-20 resin column chromatography with gradient Downregulation of progesterone biosynthesis by ABM S-M Hsia et al elution as previously described 7 and had 21 fractions assigned as A to U (Figure 1). For in vitro study, powders of adlay extracts were dissolved in DMSO to prepare stocks.…”

“…Phytochemistry studies revealed the presence of phytosterols, phenolic acids, phenolic aldehydes, lignan and flavonoids in adlay bran extract. 3,7,10,49 We have isolated some isoflavone and flavonoid phytochemicals in adlay bran extracts (unpublished data). Flavonoid phytochemicals have been shown to possess an inhibitory effect on steroidogenic enzymes in human adrenocortical H295R cells.…”

“…[4][5][6] Methanolic extracts from adlay seeds have been reported to have a moderate antioxidant effect. 7,8 Adlay also has a modulating ability to shift the balance from Th2 to Th1 dominance in the T-cell-mediated immune response and may be beneficial for the treatment of allergic disorders. 9 In addition, adlay has long been used in the folk medicine of Chinese as a nourishing food, to regulate the female endocrine system.…”

Antioxidant Properties and Total phenolic content of herbs used in post partum diet therapy in Patna (Bihar), India

“…3 Epidemiological and invitro studies suggested that plants are major constituent in antioxidant based drugs/ formulations used for the prevention of complex diseases. 4 The use of herbs in traditional way is becoming revitalized over the world. More over since modern medicine in becoming more wide spread but at a high cost.…”